J. Biol. Chem., Vol. 268, Issue 20, 14776-14780, 07, 1993
ATP synthesis energized by delta pNa and delta psi in proteoliposomes containing the F0F1-ATPase from Propionigenium modestum
O Dmitriev, G Deckers-Hebestreit and K Altendorf
Universitat Osnabruck, Fachbereich Biologie/Chemie, Arbeitsgruppe Mikrobiologie, Osnabruck, Germany.
After incorporation of the purified Na(+)-translocating F0F1-ATPase from
Propionigenium modestum into preformed phospholipid vesicles the synthesis
of ATP from ADP and inorganic phosphate could be observed under conditions
where a valinomycin-mediated K+ diffusion potential (delta psi) and/or a
Na+ concentration gradient (delta pNa) were imposed. This reaction was not
inhibited by the protonophore carbonyl cyanide
p-tri-fluoromethoxyphenylhydrazone (FCCP). Furthermore, the delta
pNa-driven ATP synthesis was stimulated by FCCP. In contrast, the addition
of the Na+/H+ antiporter monensin or of the F0F1 inhibitors
N,N'-dicyclohexylcarbodiimide and venturicidin abolished the synthesis of
ATP completely. Finally, delta pNa alone was able to elicit ATP synthesis,
when a Na+ concentration gradient of sufficient magnitude was applied. In
this case ATP synthesis occurred above a threshold level of approximately
120 mV and, furthermore, delta psi and delta pNa appear to be equivalent as
driving forces for this process. Therefore, the data provide firm evidence
for the concept that delta"mu Na+ is the primary driving force for the
synthesis of ATP in P. modestum.
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