| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 268, Issue 24, 17820-17829, 08, 1993
S Satoh, H Nishimura, AE Clark, IJ Kozka, SJ Vannucci, IA Simpson, MJ Quon, SW Cushman and GD Holman
The subcellular trafficking of tracer-tagged GLUT4 between the plasma
membranes and low-density microsomes of rat adipose cells has been studied.
Cell-surface GLUT4 have been initially tracer-tagged in the
insulin-stimulated state with the [3H]bismanose photolabel 2-N-4-(1-azi-
2,2,2-trifluoroethyl)benzoyl-1,3-bis-(D-mannos- 4-yloxy)-2- propylamine.
The half-time for internalization of tracer-tagged GLUT4 when insulin is
removed by collagenase treatment is similar to that observed for the
decrease in immunodetectable GLUT4 in the plasma membranes and the decrease
in glucose transport activity in the intact cells. In contrast,
internalization of tracer-tagged GLUT4 also occurs when cells are
maintained in the continuous presence of insulin even though the plasma
membrane level of immunodetectable GLUT4 and glucose transport activity in
the intact cells are unaltered. These data show, for the first time, that
insulin has little, if any, effect on the rate constant for GLUT4
endocytosis, but instead, primarily increases the rate constant for
exocytosis. Tracer-tagged GLUT4 that is returned to the low-density
microsomes can be restimulated with fresh insulin to recycle to the plasma
membranes and to a steady-state distribution level that is the same as that
observed in cells that are maintained in the continuous presence of
insulin. These data suggest that the cells' entire complement of GLUT4 is
involved in the recycling process. Following insulin stimulation of adipose
cells initially in the basal state, the increase in immunodetectable GLUT4
in the plasma membranes precedes the increase in accessibility of GLUT4 to
exofacial 2-N-4-(1- azi-2,2,2-trifluoroethyl)benzoyl-1,3-bis(D-mannos-4
-yloxy)-2- propylamine photolabeling, and this in turn precedes the
increase in cellular glucose transport activity. Such time course data
suggest that there may be plasma membrane intermediate states in the GLUT4
trafficking pathway. The kinetic properties of GLUT4 translocation and its
recycling have been interpreted in terms of a subcellular trafficking model
that identifies exocytosis, possibly involving- hypothetical "docking" and
"fusion" steps, as the critical site of hormone action.
Use of bismannose photolabel to elucidate insulin-regulated GLUT4 subcellular trafficking kinetics in rat adipose cells. Evidence that exocytosis is a critical site of hormone action
Experimental Diabetes, Metabolism, and Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Puthanveetil, F. Wang, G. Kewalramani, M. S. Kim, E. Hosseini-Beheshti, N. Ng, W. Lau, T. Pulinilkunnil, M. Allard, A. Abrahani, et al. Cardiac glycogen accumulation after dexamethasone is regulated by AMPK Am J Physiol Heart Circ Physiol, October 1, 2008; 295(4): H1753 - H1762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. T. Watson and J. E. Pessin Recycling of IRAP from the plasma membrane back to the insulin-responsive compartment requires the Q-SNARE syntaxin 6 but not the GGA clathrin adaptors J. Cell Sci., April 15, 2008; 121(8): 1243 - 1251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Larance, G. Ramm, and D. E. James The GLUT4 Code Mol. Endocrinol., February 1, 2008; 22(2): 226 - 233. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Williams and J. E. Pessin Mapping of R-SNARE function at distinct intracellular GLUT4 trafficking steps in adipocytes J. Cell Biol., January 28, 2008; 180(2): 375 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Muretta, I. Romenskaia, and C. C. Mastick Insulin Releases Glut4 from Static Storage Compartments into Cycling Endosomes and Increases the Rate Constant for Glut4 Exocytosis J. Biol. Chem., January 4, 2008; 283(1): 311 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. F. Pilch, R. P. Souto, L. Liu, M. P. Jedrychowski, E. A. Berg, C. E. Costello, and S. P. Gygi Cellular spelunking: exploring adipocyte caveolae J. Lipid Res., October 1, 2007; 48(10): 2103 - 2111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D'Andrea-Merrins, L. Chang, A. D. Lam, S. A. Ernst, and E. L. Stuenkel Munc18c Interaction with Syntaxin 4 Monomers and SNARE Complex Intermediates in GLUT4 Vesicle Trafficking J. Biol. Chem., June 1, 2007; 282(22): 16553 - 16566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Huang, L. M. Lifshitz, C. Jones, K. D. Bellve, C. Standley, S. Fonseca, S. Corvera, K. E. Fogarty, and M. P. Czech Insulin Stimulates Membrane Fusion and GLUT4 Accumulation in Clathrin Coats on Adipocyte Plasma Membranes Mol. Cell. Biol., May 1, 2007; 27(9): 3456 - 3469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Williams, S. W. Hicks, C. E. Machamer, and J. E. Pessin Golgin-160 Is Required for the Golgi Membrane Sorting of the Insulin-responsive Glucose Transporter GLUT4 in Adipocytes Mol. Biol. Cell, December 1, 2006; 17(12): 5346 - 5355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Hou, N. Suzuki, J. E. Pessin, and R. T. Watson A Specific Dileucine Motif Is Required for the GGA-dependent Entry of Newly Synthesized Insulin-responsive Aminopeptidase into the Insulin-responsive Compartment J. Biol. Chem., November 3, 2006; 281(44): 33457 - 33466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liu, M. P. Jedrychowski, S. P. Gygi, and P. F. Pilch Role of Insulin-dependent Cortical Fodrin/Spectrin Remodeling in Glucose Transporter 4 Translocation in Rat Adipocytes Mol. Biol. Cell, October 1, 2006; 17(10): 4249 - 4256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Jones, K. Fugo, C. Petrow-Sadowski, Y. Huang, D. C. Bertolette, I. Lisinski, S. W. Cushman, S. Jacobson, and F. W. Ruscetti Human T-Cell Leukemia Virus Type 1 (HTLV-1) and HTLV-2 Use Different Receptor Complexes To Enter T Cells. J. Virol., September 1, 2006; 80(17): 8291 - 8302. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yang and G. D. Holman Long-Term Metformin Treatment Stimulates Cardiomyocyte Glucose Transport through an AMP-Activated Protein Kinase-Dependent Reduction in GLUT4 Endocytosis Endocrinology, June 1, 2006; 147(6): 2728 - 2736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Wijesekara, A. Tung, F. Thong, and A. Klip Muscle cell depolarization induces a gain in surface GLUT4 via reduced endocytosis independently of AMPK Am J Physiol Endocrinol Metab, June 1, 2006; 290(6): E1276 - E1286. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Chen, P. Liu, G. R. Pattar, L. Tackett, P. Bhonagiri, A. B. Strawbridge, and J. S. Elmendorf Chromium Activates Glucose Transporter 4 Trafficking and Enhances Insulin-Stimulated Glucose Transport in 3T3-L1 Adipocytes via a Cholesterol-Dependent Mechanism Mol. Endocrinol., April 1, 2006; 20(4): 857 - 870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ishiki and A. Klip Minireview: Recent Developments in the Regulation of Glucose Transporter-4 Traffic: New Signals, Locations, and Partners Endocrinology, December 1, 2005; 146(12): 5071 - 5078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ishiki, V. K. Randhawa, V. Poon, L. JeBailey, and A. Klip Insulin Regulates the Membrane Arrival, Fusion, and C-terminal Unmasking of Glucose Transporter-4 via Distinct Phosphoinositides J. Biol. Chem., August 5, 2005; 280(31): 28792 - 28802. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. S. L. Thong, C. B. Dugani, and A. Klip Turning Signals On and Off: GLUT4 Traffic in the Insulin-Signaling Highway Physiology, August 1, 2005; 20(4): 271 - 284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. Lizunov, H. Matsumoto, J. Zimmerberg, S. W. Cushman, and V. A. Frolov Insulin stimulates the halting, tethering, and fusion of mobile GLUT4 vesicles in rat adipose cells J. Cell Biol., May 9, 2005; 169(3): 481 - 489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bazuine, F. Carlotti, M. J. W. E. Rabelink, J. Vellinga, R. C. Hoeben, and J. A. Maassen The p38 Mitogen-Activated Protein Kinase Inhibitor SB203580 Reduces Glucose Turnover by the Glucose Transporter-4 of 3T3-L1 Adipocytes in the Insulin-Stimulated State Endocrinology, April 1, 2005; 146(4): 1818 - 1824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Tremblay, A. Gagnon, A. Veilleux, A. Sorisky, and A. Marette Activation of the Mammalian Target of Rapamycin Pathway Acutely Inhibits Insulin Signaling to Akt and Glucose Transport in 3T3-L1 and Human Adipocytes Endocrinology, March 1, 2005; 146(3): 1328 - 1337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yang and G. D. Holman Insulin and Contraction Stimulate Exocytosis, but Increased AMP-activated Protein Kinase Activity Resulting from Oxidative Metabolism Stress Slows Endocytosis of GLUT4 in Cardiomyocytes J. Biol. Chem., February 11, 2005; 280(6): 4070 - 4078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Brozinick Jr., E. D. Hawkins, A. B. Strawbridge, and J. S. Elmendorf Disruption of Cortical Actin in Skeletal Muscle Demonstrates an Essential Role of the Cytoskeleton in Glucose Transporter 4 Translocation in Insulin-sensitive Tissues J. Biol. Chem., September 24, 2004; 279(39): 40699 - 40706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. H. Khan, E. Capilla, J. C. Hou, R. T. Watson, J. R. Smith, and J. E. Pessin Entry of Newly Synthesized GLUT4 into the Insulin-responsive Storage Compartment Is Dependent upon Both the Amino Terminus and the Large Cytoplasmic Loop J. Biol. Chem., September 3, 2004; 279(36): 37505 - 37511. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Funaki, P. Randhawa, and P. A. Janmey Separation of Insulin Signaling into Distinct GLUT4 Translocation and Activation Steps Mol. Cell. Biol., September 1, 2004; 24(17): 7567 - 7577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Malikova, J. Shi, and K. V. Kandror V-type ATPase is involved in biogenesis of GLUT4 vesicles Am J Physiol Endocrinol Metab, September 1, 2004; 287(3): E547 - E552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Govers, A. C. F. Coster, and D. E. James Insulin Increases Cell Surface GLUT4 Levels by Dose Dependently Discharging GLUT4 into a Cell Surface Recycling Pathway Mol. Cell. Biol., July 15, 2004; 24(14): 6456 - 6466. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Lu, T.-X. Sun, R. Bouley, K. Blackburn, M. McLaughlin, and D. Brown Inhibition of endocytosis causes phosphorylation (S256)-independent plasma membrane accumulation of AQP2 Am J Physiol Renal Physiol, February 1, 2004; 286(2): F233 - F243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Niu, C. Huang, Z. Nawaz, M. Levy, R. Somwar, D. Li, P. J. Bilan, and A. Klip Maturation of the Regulation of GLUT4 Activity by p38 MAPK during L6 Cell Myogenesis J. Biol. Chem., May 9, 2003; 278(20): 17953 - 17962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P. Souto, G. Vallega, J. Wharton, J. Vinten, J. Tranum-Jensen, and P. F. Pilch Immunopurification and Characterization of Rat Adipocyte Caveolae Suggest Their Dissociation from Insulin Signaling J. Biol. Chem., May 9, 2003; 278(20): 18321 - 18329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Vauquelin, Y. Michotte, I. Smolders, S. Sarre, G. Ebinger, A. Dupont, and P. Vanderheyden Cellular targets for angiotensin II fragments: pharmacological and molecular evidence Journal of Renin-Angiotensin-Aldosterone System, December 1, 2002; 3(4): 195 - 204. [Abstract] [PDF]
|
|
|
|
|
|
A. R. Sedaghat, A. Sherman, and M. J. Quon A mathematical model of metabolic insulin signaling pathways Am J Physiol Endocrinol Metab, November 1, 2002; 283(5): E1084 - E1101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kanzaki and J. E. Pessin Caveolin-associated Filamentous Actin (Cav-actin) Defines a Novel F-actin Structure in Adipocytes J. Biol. Chem., July 12, 2002; 277(29): 25867 - 25869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shigematsu, A. H. Khan, M. Kanzaki, and J. E. Pessin Intracellular Insulin-Responsive Glucose Transporter (GLUT4) Distribution But Not Insulin-Stimulated GLUT4 Exocytosis and Recycling Are Microtubule Dependent Mol. Endocrinol., May 1, 2002; 16(5): 1060 - 1068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Kupriyanova, V. Kandror, and K. V. Kandror Isolation and Characterization of the Two Major Intracellular Glut4 Storage Compartments J. Biol. Chem., March 8, 2002; 277(11): 9133 - 9138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yang, A. Hodel, and G. D. Holman Insulin and Isoproterenol Have Opposing Roles in the Maintenance of Cytosol pH and Optimal Fusion of GLUT4 Vesicles with the Plasma Membrane J. Biol. Chem., February 15, 2002; 277(8): 6559 - 6566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Al-Hasani, R. K. Kunamneni, K. Dawson, C. S. Hinck, D. Muller-Wieland, and S. W. Cushman Roles of the N- and C-termini of GLUT4 in endocytosis J. Cell Sci., January 1, 2002; 115(1): 131 - 140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Becker, L. Sevilla, E. Tomas, M. Palacin, A. Zorzano, and Y. Fischer The Endosomal Compartment Is an Insulin-Sensitive Recruitment Site for GLUT4 and GLUT1 Glucose Transporters in Cardiac Myocytes Endocrinology, December 1, 2001; 142(12): 5267 - 5276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Foster, D. Li, V. K. Randhawa, and A. Klip Insulin Accelerates Inter-endosomal GLUT4 Traffic via Phosphatidylinositol 3-Kinase and Protein Kinase B J. Biol. Chem., November 16, 2001; 276(47): 44212 - 44221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kanzaki and J. E. Pessin Insulin-stimulated GLUT4 Translocation in Adipocytes Is Dependent upon Cortical Actin Remodeling J. Biol. Chem., November 2, 2001; 276(45): 42436 - 42444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Lampson, J. Schmoranzer, A. Zeigerer, S. M. Simon, and T. E. McGraw Insulin-regulated Release from the Endosomal Recycling Compartment Is Regulated by Budding of Specialized Vesicles Mol. Biol. Cell, November 1, 2001; 12(11): 3489 - 3501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Bogan, A. E. McKee, and H. F. Lodish Insulin-Responsive Compartments Containing GLUT4 in 3T3-L1 and CHO Cells: Regulation by Amino Acid Concentrations Mol. Cell. Biol., July 15, 2001; 21(14): 4785 - 4806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yonemitsu, H. Nishimura, M. Shintani, R. Inoue, Y. Yamamoto, H. Masuzaki, Y. Ogawa, K. Hosoda, G. Inoue, T. Hayashi, et al. Troglitazone Induces GLUT4 Translocation in L6 Myotubes Diabetes, May 1, 2001; 50(5): 1093 - 1101. [Abstract] [Full Text]
|
|
|
|
|
|
L. Chamberlain, M. Graham, S Kane, J. Jackson, V. Maier, R. Burgoyne, and G. Gould The synaptic vesicle protein, cysteine-string protein, is associated with the plasma membrane in 3T3-L1 adipocytes and interacts with syntaxin 4 J. Cell Sci., January 1, 2001; 114(2): 445 - 455. [Abstract] [PDF]
|
|
|
|
|
|
M. Kawanishi, Y. Tamori, H. Okazawa, S. Araki, H. Shinoda, and M. Kasuga Role of SNAP23 in Insulin-induced Translocation of GLUT4 in 3T3-L1 Adipocytes. MEDIATION OF COMPLEX FORMATION BETWEEN SYNTAXIN4 AND VAMP2 J. Biol. Chem., March 10, 2000; 275(11): 8240 - 8247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Subtil, M. A Lampson, S. R. Keller, and T. E. McGraw Characterization of the Insulin-regulated Endocytic Recycling Mechanism in 3T3-L1 Adipocytes Using a Novel Reporter Molecule J. Biol. Chem., February 18, 2000; 275(7): 4787 - 4795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Garza and M. J. Birnbaum Insulin-responsive Aminopeptidase Trafficking in 3T3-L1 Adipocytes J. Biol. Chem., January 28, 2000; 275(4): 2560 - 2567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D Malide, G Ramm, S. Cushman, and J. Slot Immunoelectron microscopic evidence that GLUT4 translocation explains the stimulation of glucose transport in isolated rat white adipose cells J. Cell Sci., January 12, 2000; 113(23): 4203 - 4210. [Abstract] [PDF]
|
|
|
|
|
|
M. Lampson, A Racz, S. Cushman, and T. McGraw Demonstration of insulin-responsive trafficking of GLUT4 and vpTR in fibroblasts J. Cell Sci., January 11, 2000; 113(22): 4065 - 4076. [Abstract] [PDF]
|
|
|
|
|
|
S Martin, C. Millar, C. Lyttle, T Meerloo, B. Marsh, G. Gould, and D. James Effects of insulin on intracellular GLUT4 vesicles in adipocytes: evidence for a secretory mode of regulation J. Cell Sci., January 10, 2000; 113(19): 3427 - 3438. [Abstract] [PDF]
|
|
|
|
|
|
K. V. Kandror Insulin Regulation of Protein Traffic in Rat Adipose Cells J. Biol. Chem., September 3, 1999; 274(36): 25210 - 25217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F. Hausdorff, D. C. Fingar, K. Morioka, L. A. Garza, E. L. Whiteman, S. A. Summers, and M. J. Birnbaum Identification of Wortmannin-sensitive Targets in 3T3-L1 Adipocytes. DISSOCIATION OF INSULIN-STIMULATED GLUCOSE UPTAKE AND GLUT4 TRANSLOCATION J. Biol. Chem., August 27, 1999; 274(35): 24677 - 24684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Bogan and H. F. Lodish Two Compartments for Insulin-stimulated Exocytosis in 3T3-L1 Adipocytes Defined by Endogenous ACRP30 and GLUT4 J. Cell Biol., August 9, 1999; 146(3): 609 - 620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. El-Jack, K. V. Kandror, and P. F. Pilch The Formation of an Insulin-responsive Vesicular Cargo Compartment Is an Early Event in 3T3-L1 Adipocyte Differentiation Mol. Biol. Cell, May 1, 1999; 10(5): 1581 - 1594. [Abstract] [Full Text]
|
|
|
|
 |
|
 B Scholtka, F Stumpel, and K Jungermann Acute increase, stimulated by prostaglandin E2, in glucose absorption via the sodium dependent glucose transporter-1 in rat intestine Gut, April 1, 1999; 44(4): 490 - 496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Pessin, D. C. Thurmond, J. S. Elmendorf, K. J. Coker, and S. Okada Molecular Basis of Insulin-stimulated GLUT4 Vesicle Trafficking. LOCATION! LOCATION! LOCATION! J. Biol. Chem., January 29, 1999; 274(5): 2593 - 2596. [Full Text] [PDF]
|
|
|
