HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by VanRenterghem, B. Articles by Maller, J. L. Search for Related Content PubMed PubMed Citation Articles by VanRenterghem, B. Articles by Maller, J. L. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 268, Issue 27, 19935-19938, 09, 1993

Reconstitution of p21ras-dependent and -independent mitogen-activated protein kinase activation in a cell-free system

B VanRenterghem, JB Gibbs and JL Maller
Howard Hughes Medical Institute, University of Colorado School of Medicine, Denver 80262.

In Xenopus oocytes, mitogen-activated protein (MAP) kinase can be activated by progesterone treatment or by microinjection of cyclin A, both of which lead to activation of the cdc2 protein kinase. The tyrosine kinase pp60v-src has previously been shown to accelerate progesterone-induced oocyte maturation and to increase the phosphorylation of ribosomal protein S6 by pp90rsk, most likely by activating MAP kinase. In extracts of resting oocytes, MAP kinase kinase and MAP kinase were activated by addition of pp60v-src or cyclin A. Activation by pp60v-src was blocked by a dominant-negative p21ras protein (RAST), but activation by cyclin A/cdc2 was unaffected. Thus these two pathways that converge at MAP kinase kinase but are clearly divergent upstream of a p21ras-dependent step can be studied in a cell- free system.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				B. J. Tunquist and J. L. Maller Under arrest: cytostatic factor (CSF)-mediated metaphase arrest in vertebrate eggs Genes & Dev., March 15, 2003; 17(6): 683 - 710. [Full Text] [PDF]

				S. D. Gross, A. L. Lewellyn, and J. L. Maller A Constitutively Active Form of the Protein Kinase p90Rsk1 Is Sufficient to Trigger the G2/M Transition in Xenopus Oocytes J. Biol. Chem., November 30, 2001; 276(49): 46099 - 46103. [Abstract] [Full Text] [PDF]

				E. L. Howard, A. Charlesworth, J. Welk, and A. M. MacNicol The Mitogen-Activated Protein Kinase Signaling Pathway Stimulates Mos mRNA Cytoplasmic Polyadenylation during Xenopus Oocyte Maturation Mol. Cell. Biol., March 1, 1999; 19(3): 1990 - 1999. [Abstract] [Full Text] [PDF]

				T. L. Williamson, L. I. Bruijn, Q. Zhu, K. L. Anderson, S. D. Anderson, J.-P. Julien, and D. W. Cleveland Absence of neurofilaments reduces the selective vulnerability of motor neurons and slows disease caused by a familial amyotrophic lateral sclerosis-linked superoxide dismutase 1 mutant PNAS, August 4, 1998; 95(16): 9631 - 9636. [Abstract] [Full Text] [PDF]

				J Moos, G. Kopf, and R. Schultz Cycloheximide-induced activation of mouse eggs: effects on cdc2/cyclin B and MAP kinase activities J. Cell Sci., January 4, 1996; 109(4): 739 - 748. [Abstract] [PDF]

				O Haccard, B Sarcevic, A Lewellyn, R Hartley, L Roy, T Izumi, E Erikson, and J. Maller Induction of metaphase arrest in cleaving Xenopus embryos by MAP kinase Science, November 19, 1993; 262(5137): 1262 - 1265. [Abstract] [PDF]