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J. Biol. Chem., Vol. 268, Issue 29, 21674-21679, Oct, 1993
BF Lin, RF Huang and B Shane
Wild-type Chinese hamster ovary (CHO) cells and CHO cell transfectants
expressing human folylpoly-gamma-glutamate synthetase (FPGS) activity
contain mitochondrial FPGS activity of higher specific activity than the
cytosolic isozyme. Expression of mitochondrial FPGS activity is required
for folate accumulation by mitochondria. The mitochondrial folate pool in
CHO cells is not in equilibrium with the cytosolic pool and contains
folylpolyglutamates of longer glutamate chain length than cytosolic
folates. The inability of AUX-coli, a CHO cell expressing high levels of
Escherichia coli FPGS activity and containing pteroyltriglutamate, to
support glycine synthesis is due to a lack of mitochondrial FPGS activity.
AUX-coli cells lack mitochondrial folate despite containing high levels of
cytosolic folate.
Regulation of folate and one-carbon metabolism in mammalian cells. III. Role of mitochondrial folylpoly-gamma-glutamate synthetase
Department of Nutritional Sciences, University of California, Berkeley 94720.
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