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J. Biol. Chem., Vol. 268, Issue 29, 21693-21700, 10, 1993
BA Lollo, KW Chan, EM Hanson, VT Moy and AA Brian
Lymphocytes activated by antigen receptor cross-linking or phorbol esters
adhere avidly to surfaces bearing intercellular adhesion molecule 1
(ICAM-1) through the adhesion receptor lymphocyte function- associated
antigen 1 (LFA-1). It is not known whether avid adhesion by stimulated
lymphocytes is due to higher affinity binding of ICAM-1 or due solely to
post-receptor mechanisms. We have used a recombinant, soluble form of the
ICAM-1 molecule to measure the affinity of binding to LFA-1 on unstimulated
T cells and T cells stimulated with phorbol esters. The affinity was found
to be too low for direct measurements, requiring instead the use of
competition protocols in which ICAM-1 competes for binding with
radiolabeled Fab from a monoclonal antibody specific for LFA-1. By analysis
of the equilibrium and kinetics of competitive binding, we found that the
affinity on unstimulated T cells is very low, about 100 microM. Activation
of the T cells by phorbol esters caused a small increase in average binding
affinity. Further analysis suggested that the change in average affinity
reflected the conversion of a fraction of LFA-1 molecules to a state with a
200-fold higher affinity.
Direct evidence for two affinity states for lymphocyte function- associated antigen 1 on activated T cells [published erratum appears in J Biol Chem 1994 Apr 1;269(13):10184]
Department of Chemistry, University of California at San Diego, La Jolla 92093-0063.
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|
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|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
 J. E. Chin, G. E. Winterrowd, C. A. Hatfield, J. R. Brashler, R. L. Griffin, S. L. Vonderfecht, K. P. Kolbasa, S. F. Fidler, K. L. Shull, R. F. Krzesicki, et al. Involvement of Intercellular Adhesion Molecule-1 in the Antigen-induced Infiltration of Eosinophils and Lymphocytes into the Airways in a Murine Model of Pulmonary Inflammation Am. J. Respir. Cell Mol. Biol., February 1, 1998; 18(2): 158 - 167. [Abstract] [Full Text]
|
|
|
|
|
|
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|
|
|
|
|
|
R. Knorr and M. L. Dustin The Lymphocyte Function-associated Antigen 1 I Domain Is a Transient Binding Module for Intercellular Adhesion Molecule (ICAM)-1 and ICAM-3 in Hydrodynamic Flow J. Exp. Med., August 29, 1997; 186(5): 719 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Loh, W. Qi, G. Vilaire, and J. S. Bennett Effect of Cytoplasmic Domain Mutations on the Agonist-stimulated Ligand Binding Activity of the Platelet Integrin alpha IIbbeta 3 J. Biol. Chem., November 22, 1996; 271(47): 30233 - 30241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Faull, J. Wang, D. I. Leavesley, W. Puzon, G. R. Russ, D. Vestweber, and Y. Takada A Novel Activating Anti-beta 1 Integrin Monoclonal Antibody Binds to the Cysteine-rich Repeats in the beta 1 Chain J. Biol. Chem., October 11, 1996; 271(41): 25099 - 25106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Luque, M. Gómez, W. Puzon, Y. Takada, F. Sánchez-Madrid, and C. Cabañas Activated Conformations of Very Late Activation Integrins Detected by a Group of Antibodies (HUTS) Specific for a Novel Regulatory Region(355-425) of the Common beta1 Chain J. Biol. Chem., May 10, 1996; 271(19): 11067 - 11075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Petruzzelli, M. Takami, and R. Herrera Adhesion through the Interaction of Lymphocyte Function-associated Antigen-1 with Intracellular Adhesion Molecule-1 Induces Tyrosine Phosphorylation of p130[IMAGE] and Its Association with c-CrkII J. Biol. Chem., March 29, 1996; 271(13): 7796 - 7801. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. Hunt III, E. S. Harris, S.-A. Kellermann, and Y. Shimizu T-Lymphocyte Interactions With Endothelium and Extracellular Matrix Critical Reviews in Oral Biology & Medicine, January 1, 1996; 7(1): 59 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Yednock, C. Cannon, C. Vandevert, E. G. Goldbach, G. Shaw, D. K. Ellis, C. Liaw, L. C. Fritz, and L. I. Tanner alpha(4)beta(1)Integrin-dependent Cell Adhesion Is Regulated by a Low Affinity Receptor Pool That Is Conformationally Responsive to Ligand J. Biol. Chem., December 1, 1995; 270(48): 28740 - 28750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Loh, K. Beaverson, G. Vilaire, W. Qi, M. Poncz, and J. S. Bennett Agonist-stimulated Ligand Binding by the Platelet Integrin alphaIIbbeta3 in a Lymphocyte Expression System J. Biol. Chem., August 4, 1995; 270(31): 18631 - 18636. [Abstract] [Full Text] [PDF]
|
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T.-Q. Cai and S. D. Wright Energetics of Leukocyte Integrin Activation J. Biol. Chem., |