JBC Connect with Cosmo for Collagen Detection

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kong, C. T.
Right arrow Articles by Lever, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kong, C. T.
Right arrow Articles by Lever, J. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J. Biol. Chem., Vol. 268, Issue 3, 1509-1512, Jan, 1993

Cloning and expression of a mammalian Na+/amino acid cotransporter with sequence similarity to Na+/glucose cotransporters

CT Kong, SF Yet and JE Lever
Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77225.

We describe the full-length sequence and functional expression of a cDNA cloned from LLC-PK1 cells, which appears to encode a mammalian Na(+)-dependent neutral amino acid transporter with properties characteristic of system A. This sequence, designated SAAT1, is 76% identical and 89% similar in amino acid sequence to the Na(+)-dependent glucose transporter SGLT1 of the same species. A leucine zipper region was detected in both SAAT1 and SGLT1. The message for SAAT1 was a single 2.4-kilobase species in kidney, but mRNA species of 2.4 and 3.7 kilobases were observed in LLC-PK1 cells as well as in intestine. Transcripts were also found in spleen, liver, and muscle. Expression of SAAT1 in COS-7 cells resulted in increased levels of Na(+)-dependent uptake of 2-(methylamino)isobutyric acid, a specific substrate for the system A amino acid transporter. Uptake due to cDNA expression was inhibited by a range of amino acids that are transported by system A and exhibited a km of 0.8 +/- 0.2 mM. These results suggest that the system A amino acid transporter is closely related to the Na+/glucose transporter SGLT1.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J DAIRY SCIHome page
F.-Q. Zhao and A. F. Keating
Expression and Regulation of Glucose Transporters in the Bovine Mammary Gland
J Dairy Sci, June 1, 2007; 90(13_suppl): E76 - E86.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
F.-Q. Zhao, Y.-C. Zheng, E. H. Wall, and T. B. McFadden
Cloning and Expression of Bovine Sodium/Glucose Cotransporters
J Dairy Sci, January 1, 2005; 88(1): 182 - 194.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Diez-Sampedro, B. A. Hirayama, C. Osswald, V. Gorboulev, K. Baumgarten, C. Volk, E. M. Wright, and H. Koepsell
A glucose sensor hiding in a family of transporters
PNAS, September 30, 2003; 100(20): 11753 - 11758.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
C. Bingham, S. Ellard, A. J. Nicholls, C. A. Pennock, J. Allen, A. J. James, S. C. Satchell, M. B. Salzmann, and A. T. Hattersley
The Generalized Aminoaciduria Seen in Patients With Hepatocyte Nuclear Factor-1{alpha} Mutations Is a Feature of All Patients With Diabetes and Is Associated With Glucosuria
Diabetes, September 1, 2001; 50(9): 2047 - 2052.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. Diez-Sampedro, S. Eskandari, E. M. Wright, and B. A. Hirayama
Na+-to-sugar stoichiometry of SGLT3
Am J Physiol Renal Physiol, February 1, 2001; 280(2): F278 - F282.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
E. M. Wright
Renal Na+-glucose cotransporters
Am J Physiol Renal Physiol, January 1, 2001; 280(1): F10 - F18.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
E. Briasoulis, I. Judson, N. Pavlidis, P. Beale, J. Wanders, Y. Groot, G. Veerman, M. Schuessler, G. Niebch, K. Siamopoulos, et al.
Phase I Trial of 6-Hour Infusion of Glufosfamide, a New Alkylating Agent With Potentially Enhanced Selectivity for Tumors That Overexpress Transmembrane Glucose Transporters: A Study of the European Organization for Research and Treatment of Cancer Early Clinical Studies Group
J. Clin. Oncol., October 20, 2000; 18(20): 3535 - 3544.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Q. Yang, Y. Tian, J. Wada, N. Kashihara, E. Wallner, D. Peterson, and Y. S. Kanwar
Expression characteristics and relevance of sodium glucose cotransporter-1 in mammalian renal tubulogenesis
Am J Physiol Renal Physiol, October 1, 2000; 279(4): F765 - F777.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Veyhl, K. Wagner, C. Volk, V. Gorboulev, K. Baumgarten, W.-M. Weber, M. Schaper, B. Bertram, M. Wiessler, and H. Koepsell
Transport of the new chemotherapeutic agent beta -D-glucosylisophosphoramide mustard (D-19575) into tumor cells is mediated by the Na+-D-glucose cotransporter SAAT1
PNAS, March 17, 1998; 95(6): 2914 - 2919.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Mackenzie, D. D.F. Loo, M. Panayotova-Heiermann, and E. M. Wright
Biophysical Characteristics of the Pig Kidney Na+/Glucose Cotransporter SGLT2 Reveal a Common Mechanism for SGLT1 and SGLT2
J. Biol. Chem., December 20, 1996; 271(51): 32678 - 32683.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Kekuda, P. D. Prasad, Y.-J. Fei, V. Torres-Zamorano, S. Sinha, T. L. Yang-Feng, F. H. Leibach, and V. Ganapathy
Cloning of the Sodium-dependent, Broad-scope, Neutral Amino Acid Transporter Bo from a Human Placental Choriocarcinoma Cell Line
J. Biol. Chem., August 2, 1996; 271(31): 18657 - 18661.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Panayotova-Heiermann, D. D. F. Loo, C.-T. Kong, J. E. Lever, and E. M. Wright
Sugar Binding to Na[IMAGE]/Glucose Cotransporters Is Determined by the Carboxyl-terminal Half of the Protein
J. Biol. Chem., April 26, 1996; 271(17): 10029 - 10034.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. You, W.-S. Lee, E. J. G. Barros, Y. Kanai, T.-L. Huo, S. Khawaja, R. G. Wells, S. K. Nigam, and M. A. Hediger
Molecular Characteristics of Na[IMAGE]-coupled Glucose Transporters in Adult and Embryonic Rat Kidney
J. Biol. Chem., December 8, 1995; 270(49): 29365 - 29371.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Peng and J. E. Lever
Post-transcriptional Regulation of Na[IMAGE]/Glucose Cotransporter (SGTL1) Gene Expression in LLC-PK(1) Cells
J. Biol. Chem., September 1, 1995; 270(35): 20536 - 20542.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Pan and B. R. Stevens
Differentiation- and Protein Kinase C-dependent Regulation of Alanine Transport via System B
J. Biol. Chem., February 24, 1995; 270(8): 3582 - 3587.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Diez-Sampedro, E. M. Wright, and B. A. Hirayama
Residue 457 Controls Sugar Binding and Transport in the Na+/Glucose Cotransporter
J. Biol. Chem., December 21, 2001; 276(52): 49188 - 49194.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1993 by the American Society for Biochemistry and Molecular Biology.