| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 268, Issue 30, 22347-22352, Oct, 1993
KP Hooper, R Padmanabhan and KE Ebner
A clone of the extracellular domain of the rat liver prolactin receptor was
generated by the RNA-based polymerase chain reaction, and the NH2- terminal
210 amino acids were expressed in HeLa cells using a vaccinia virus/T7
hybrid expression system. The protein was isolated from serum- free culture
medium directly by chromatography on an ovine prolactin affinity column and
yielded approximately 1.5 mg of protein/liter of suspension culture. The
extracellular domain of the rat prolactin receptor inhibited the ovine
prolactin-dependent mitogenesis of rat lymphoma Nb2 cells with an IC50 of
7.1 pM and bound 125I-labeled ovine prolactin with a Kd of 1.21 +/- 0.19
nM. In contrast, the binding of the 125I-labeled extracellular domain to
ovine prolactin exhibited positive cooperativity with a Hill coefficient of
1.73. High pressure gel filtration chromatography was used to demonstrate
the formation of a complex consisting of one molecule of ovine prolactin
and two molecules of the extracellular domain of the rat prolactin
receptor. Complex formation occurred with human growth hormone, but not
with ovine growth hormone, a non-lactogen.
Expression of the extracellular domain of the rat liver prolactin receptor and its interaction with ovine prolactin
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66160-7421.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Bjurulf, O. Toffia, G. Selstam, and J. I. Olofsson Luteolysis Induced by a Prostaglandin F2{alpha} Analogue Occurs Independently of Prolactin in the Rat Biol Reprod, July 1, 1998; 59(1): 17 - 21. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Bole-Feysot, V. Goffin, M. Edery, N. Binart, and P. A. Kelly Prolactin (PRL) and Its Receptor: Actions, Signal Transduction Pathways and Phenotypes Observed in PRL Receptor Knockout Mice Endocr. Rev., June 1, 1998; 19(3): 225 - 268. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. J. Berlanga, J. P. Garcia-Ruiz, M. Perrot-Applanat, P. A. Kelly, and M. Edery The Short Form of The Prolactin (PRL) Receptor Silences PRL Induction of the {beta}-Casein Gene Promoter Mol. Endocrinol., September 1, 1997; 11(10): 1449 - 1457. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Gertler, J. Grosclaude, C. J. Strasburger, S. Nir, and J. Djiane Real-time Kinetic Measurements of the Interactions between Lactogenic Hormones and Prolactin-Receptor Extracellular Domains from Several Species Support the Model of Hormone-induced Transient Receptor Dimerization J. Biol. Chem., October 4, 1996; 271(40): 24482 - 24491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Goffin, S. Kinet, F. Ferrag, N. Binart, J. A. Martial, and P. A. Kelly Antagonistic Properties of Human Prolactin Analogs That Show Paradoxical Agonistic Activity in the Nb2 Bioassay J. Biol. Chem., July 12, 1996; 271(28): 16573 - 16579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Vashdi-Elberg, N. R. Staten, E. Sakal, R. E. McKinnie, J. Djiane, G. G. Krivi, and A. Gertler Selective Modification of Recombinant Bovine Placental Lactogen by Site-directed Mutagenesis at Its C Terminus J. Biol. Chem., March 8, 1996; 271(10): 5558 - 5564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Hiraoka, H. Anaguchi, A. Asakura, and Y. Ota Requirement for the Immunoglobulin-like Domain of Granulocyte Colony-stimulating Factor Receptor in Formation of a 2:1 Receptor-Ligand Complex J. Biol. Chem., October 27, 1995; 270(43): 25928 - 25934. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
