HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chiesa, G. Articles by Hobbs, H. H. Search for Related Content PubMed PubMed Citation Articles by Chiesa, G. Articles by Hobbs, H. H. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 268, Issue 32, 23747-23750, Nov, 1993

Expression of human apolipoprotein B100 in transgenic mice. Editing of human apolipoprotein B100 mRNA

G Chiesa, DF Johnson, Z Yao, TL Innerarity, RW Mahley, SG Young, RH Hammer and HH Hobbs
Department of Molecular Genetics, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235.

Apolipoprotein B (apoB) is a large glycoprotein that circulates in plasma as a major constituent of numerous lipoproteins. ApoB exists in two forms: apoB48 and apoB100. ApoB48 is identical in sequence to the N- terminal region of apoB100 and is generated by sequence-specific mRNA editing of the apoB100 transcript. Here, we describe the development of a line of mice expressing a human apoB transgene driven by promoter/enhancer sequences from the transthyretin gene. In these mice, immunodetectable human apoB100 is synthesized by the liver, kidney, and brain. Human apoB100 is found in low concentration (approximately 0.1 mg/dl) in the plasma of the transgenic mice and circulates in the low density lipoprotein fraction. The hepatic human apoB100 transcripts undergo mRNA editing at only slightly lower efficiency than the endogenous mouse apoB100 message. Therefore, there is no absolute species specificity to the apoB100 mRNA editing process.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. Singh, O. A. Batuman, H. O. Akman, M. H. Kedees, V. Vakil, and M. M. Hussain Differential, Tissue-specific, Transcriptional Regulation of Apolipoprotein B Secretion by Transforming Growth Factor beta J. Biol. Chem., October 11, 2002; 277(42): 39515 - 39524. [Abstract] [Full Text] [PDF]

				E. Voyiaziakis, C. Ko, S. M. O'Rourke, and L.-S. Huang Genetic control of hepatic apoB-100 secretion in human apoB transgenic mouse strains J. Lipid Res., November 1, 1999; 40(11): 2004 - 2012. [Abstract] [Full Text]

				D. M. Stafforini, L. W. Tjoelker, S. P. A. McCormick, D. Vaitkus, T. M. McIntyre, P. W. Gray, S. G. Young, and S. M. Prescott Molecular Basis of the Interaction between Plasma Platelet-activating Factor Acetylhydrolase and Low Density Lipoprotein J. Biol. Chem., March 12, 1999; 274(11): 7018 - 7024. [Abstract] [Full Text] [PDF]

				E. Kim and S. G. Young Genetically modified mice for the study of apolipoprotein B J. Lipid Res., April 1, 1998; 39(4): 703 - 723. [Abstract] [Full Text] [PDF]

				S. Yamanaka, K. S. Poksay, D. M. Driscoll, and T. L. Innerarity Hyperediting of Multiple Cytidines of Apolipoprotein B mRNA by APOBEC-1 Requires Auxiliary Protein(s) but Not a Mooring Sequence Motif J. Biol. Chem., May 10, 1996; 271(19): 11506 - 11510. [Abstract] [Full Text] [PDF]