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J. Biol. Chem., Vol. 268, Issue 32, 23784-23787, Nov, 1993
G Schiavo, O Rossetto, S Catsicas, P Polverino de Laureto, BR DasGupta, F Benfenati and C Montecucco
Botulinum neurotoxins are metalloproteins with one zinc atom bound to the
zinc binding motif of zinc endopeptidases. Here we show that botulinum
neurotoxin serotypes A, D, and E are zinc endoproteases specific for
components of the synaptic vesicle docking and fusion complex. Serotypes A
and E cleave SNAP-25, a 25-kDa protein of the synaptic terminal, while
serotype D is specific for VAMP/synaptobrevin, a membrane protein of
synaptic vesicles. Both rat brain VAMP isoforms are cleaved at a single
Lys-Leu peptide bond. The proteolytic activity of these neurotoxins is
inhibited by EDTA and captopril.
Identification of the nerve terminal targets of botulinum neurotoxin serotypes A, D, and E
Centro Consiglio Nazionale delle Ricerche Biomembrane, Universita di Padova, Italy.
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R. Pellizzari, O. Rossetto, L. Lozzi, S. Giovedi', E. Johnson, C. C. Shone, and C. Montecucco Structural Determinants of the Specificity for Synaptic Vesicle-associated Membrane Protein/Synaptobrevin of Tetanus and Botulinum Type B and G Neurotoxins J. Biol. Chem., August 23, 1996; 271(34): 20353 - 20358. [Abstract] [Full Text] [PDF]
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A. V. Ferrer-Montiel, J. M. Canaves, B. R. DasGupta, M. C. Wilson, and M. Montal Tyrosine Phosphorylation Modulates the Activity of Clostridial Neurotoxins J. Biol. Chem., August 2, 1996; 271(31): 18322 - 18325. [Abstract] [Full Text] [PDF]
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E. J. Hess, K. A. Collins, and M. C. Wilson Mouse Model of Hyperkinesis Implicates SNAP-25 in Behavioral Regulation J. Neurosci., May 1, 1996; 16(9): 3104 - 3111. [Abstract] [Full Text] [PDF]
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L. C. Williamson, J. L. Halpern, C. Montecucco, J. E. Brown, and E. A. Neale Clostridial Neurotoxins and Substrate Proteolysis in Intact Neurons J. Biol. Chem., March 29, 1996; 271(13): 7694 - 7699. [Abstract] [Full Text] [PDF]
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G. Schiavo, C. C. Shone, M. K. Bennett, R. H. Scheller, and C. Montecucco Botulinum Neurotoxin Type C Cleaves a Single Lys-Ala Bond within the Carboxyl-terminal Region of Syntaxins J. Biol. Chem., May 5, 1995; 270(18): 10566 - 10570. [Abstract] [Full Text] [PDF]
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E. Papini, O. Rossetto, and D. F. Cutler Vesicle-associated Membrane Protein (VAMP)/Synaptobrevin-2 Is Associated with Dense Core Secretory Granules in PC12 Neuroendocrine Cells J. Biol. Chem., January 20, 1995; 270(3): 1332 - 1336. [Abstract] [Full Text] [PDF]
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