J. Biol. Chem., Vol. 268, Issue 32, 24427-24431, 11, 1993
Murine cortactin is phosphorylated in response to fibroblast growth factor-1 on tyrosine residues late in the G1 phase of the BALB/c 3T3 cell cycle
X Zhan, X Hu, B Hampton, WH Burgess, R Friesel and T Maciag
Department of Molecular Biology, Holland Laboratory, American Red Cross, Rockville, Maryland 20855.
We have previously reported that BALB/c 3T3 cells require a prolonged
exposure to fibroblast growth factor (FGF)-1 for the stimulation of maximal
DNA synthesis, and this event correlates with the tyrosine phosphorylation
of novel proteins late in G1 including a protein termed p80/p85 (Zhan, X.,
Hu, X., Friesel, R., and Maciag, T. (1993) J. Biol. Chem. 268, 9611-9620).
We have purified, sequenced, and cloned the cDNA encoding p80/p85 and
report that it is the murine homolog of the chicken cortactin gene and a
member of the human hematopoietic specific- 1 gene family. Immunochemical
analysis of m-cortactin-tyrosine phosphorylation in response to FGF-1
demonstrates a biphasic phosphorylation pattern both as a weak
immediate-early and strong mid to late G1 response protein. Because the
chicken cortactin gene was originally isolated as a substrate for v-Src,
FGF-1 may influence the enzymatic activity of other cell-associated
tyrosine kinases which utilize p80/p85 (cortactin) as a polypeptide
substrate.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
