| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 268, Issue 5, 3739-3746, Feb, 1993
L Yang, AM Camoratto, G Baffy, S Raj, DR Manning and JR Williamson
Hepatocytes were established in tissue culture in order to study the
effects of pertussis toxin (PT) on epidermal growth factor (EGF)- mediated
cellular responses under in vitro conditions. EGF caused a 3- fold increase
of myo-inositol 1,4,5-trisphosphate (Ins-1,4,5-P3) mass and a 50% increase
of diacylglycerol mass within the first minute, with the change of
diacylglycerol content being 100-fold greater than that of Ins-1,4,5-P3.
Diacylglycerol, but not Ins-1,4,5-P3, continued to accumulate over several
hours, indicating that EGF increased the hydrolysis of lipids other than
phosphatidylinositol 4,5-bisphosphate (PIP2). EGF increased
phosphoinositide-specific phospholipase C-gamma (PLC-gamma) tyrosine
phosphorylation within 1 min, but no effect was observed with vasopressin,
insulin, or glucagon after 5 min. EGF also caused a rapid, tyrosine
kinase-dependent association of G(i) alpha with PLC-gamma, which was
maximal within 10 min. In contrast to our previous data on fresh
hepatocytes, PT had no effect on the EGF-induced tyrosine phosphorylation
of PLC-gamma, although Ins-1,4,5-P3 and diacylglycerol production were
inhibited. The role of G-proteins in EGF signaling was investigated further
by microinjection of G alpha antibodies into single fura-2-loaded
hepatocytes. Anti-G(i) alpha (common) antibodies prevented EGF-induced but
not vasopressin-induced Ca2+ transients. These results strengthen previous
observations that a PT-sensitive G-protein is involved in EGF-mediated
phospholipid metabolism in hepatocytes and show that tyrosine
phosphorylation of PLC- gamma is an insufficient signal for activation of
PIP2 hydrolysis.
Epidermal growth factor-mediated signaling of G(i)-protein to activation of phospholipases in rat-cultured hepatocytes
Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia 19104.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Zagar, G. Chaumaz, and M. Lieberherr Signaling Cross-talk from G{beta}4 Subunit to Elk-1 in the Rapid Action of Androgens J. Biol. Chem., January 23, 2004; 279(4): 2403 - 2413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Freedman, L. K. Kim, J. P. Murray, S. T. Exum, L. Brian, J.-H. Wu, and K. Peppel Phosphorylation of the Platelet-derived Growth Factor Receptor-beta and Epidermal Growth Factor Receptor by G Protein-coupled Receptor Kinase-2. MECHANISMS FOR SELECTIVITY OF DESENSITIZATION J. Biol. Chem., December 6, 2002; 277(50): 48261 - 48269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X Shi, S Amindari, K Paruchuru, D Skalla, H Burkin, B. Shur, and D. Miller Cell surface beta-1,4-galactosyltransferase-I activates G protein-dependent exocytotic signaling Development, January 3, 2001; 128(5): 645 - 654. [Abstract] [PDF]
|
|
|
|
 |
|
 M.-S. Huang, O. A. Adebanjo, E. Awumey, G. Biswas, A. Koval, B. R. Sodam, L. Sun, B. S. Moonga, J. Epstein, S. Goldstein, et al. IP3, IP3 receptor, and cellular senescence Am J Physiol Renal Physiol, April 1, 2000; 278(4): F576 - F584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hallak, A. E. M. Seiler, J. S. Green, B. N. Ross, and R. Rubin Association of Heterotrimeric Gi with the Insulin-like Growth Factor-I Receptor. RELEASE OF Gbeta gamma SUBUNITS UPON RECEPTOR ACTIVATION J. Biol. Chem., January 28, 2000; 275(4): 2255 - 2258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Cortese Rat liver GTP-binding proteins mediate changes in mitochondrial membrane potential and organelle fusion Am J Physiol Cell Physiol, March 1, 1999; 276(3): C611 - C620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Halse, J. J. Rochford, J. G. McCormack, J. R. Vandenheede, B. A. Hemmings, and S. J. Yeaman Control of Glycogen Synthesis in Cultured Human Muscle Cells J. Biol. Chem., January 8, 1999; 274(2): 776 - 780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. I. Krieger-Brauer, P. K. Medda, and H. Kather Insulin-induced Activation of NADPH-dependent H2O2 Generation in Human Adipocyte Plasma Membranes Is Mediated by Galpha i2 J. Biol. Chem., April 11, 1997; 272(15): 10135 - 10143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sun, Z. Chen, H. Poppleton, K. Scholich, J. Mullenix, G. J. Weipz, D. L. Fulgham, P. J. Bertics, and T. B. Patel The Juxtamembrane, Cytosolic Region of the Epidermal Growth Factor Receptor Is Involved in Association with alpha -Subunit of Gs J. Biol. Chem., February 28, 1997; 272(9): 5413 - 5420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Barr, L. F. Brass, and D. R. Manning Reconstitution of Receptors and GTP-binding Regulatory Proteins (G Proteins) in Sf9 Cells. A DIRECT EVALUATION OF SELECTIVITY IN RECEPTOR·G PROTEIN COUPLING J. Biol. Chem., January 24, 1997; 272(4): 2223 - 2229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Pietruck, A. Moritz, M. Montemurro, A. Sell, S. Busch, D. Rosskopf, S. Virchow, H. Esche, N. Brockmeyer, K. H. Jakobs, et al. Selectively Enhanced Cellular Signaling by Gi Proteins in Essential Hypertension: G{alpha}i2, G{alpha}i3, Gß1, and Gß2 Are Not Mutated Circ. Res., November 1, 1996; 79(5): 974 - 983. [Abstract] [Full Text]
|
|
|
|
|
|
S. Aznavoorian, M. L. Stracke, J. Parsons, J. McClanahan, and L. A. Liotta Integrin alpha(v)beta(3) Mediates Chemotactic and Haptotactic Motility in Human Melanoma Cells through Different Signaling Pathways J. Biol. Chem., February 9, 1996; 271(6): 3247 - 3254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Berven, M. F. Crouch, F. Katsis, B. E. Kemp, L. M. Harland, and G. J. Barritt Evidence That the Pertussis Toxin-sensitive Trimeric GTP-binding Protein G[IMAGE] Is Required for Agonist- and Store-activated Ca[IMAGE] Inflow in Hepatocytes J. Biol. Chem., October 27, 1995; 270(43): 25893 - 25897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Abrams, H. Wu, W. Zhao, E. Belmonte, D. White, and L. F. Brass Pleckstrin Inhibits Phosphoinositide Hydrolysis Initiated by G-protein-coupled and Growth Factor Receptors J. Biol. Chem., June 16, 1995; 270(24): 14485 - 14492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Guillard, S. Chretien, R. Jockers, S. Fichelson, P. Mayeux, and V. Duprez Coupling of Heterotrimeric Gi Proteins to the Erythropoietin Receptor J. Biol. Chem., January 12, 2001; 276(3): 2007 - 2014. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
