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J. Biol. Chem., Vol. 269, Issue 1, 55-58, 01, 1994
H Jiang, Y Wang and MR Kanost
We have isolated 38 cDNA clones for serpins from a lepidopteran insect,
Manduca sexta, and have found that they are identical in sequence, except
for a region encoding the carboxyl-terminal 40-45 residues, which includes
the reactive center. Among these clones, there are 11 variants of the
reactive center region, each encoded by a different version of the ninth
exon in the serpin gene. Thus, evolution of this insect serpin gene has
resulted from duplication and sequence divergence of only the exon encoding
the reactive site. Alternative pre- mRNA splicing then generates inhibitor
diversity and the potential to regulate a variety of proteinases, using the
same protein framework joined to different reactive site region cassettes.
Mutually exclusive exon use and reactive center diversity in insect serpins
Department of Biochemistry, Kansas State University, Manhattan 66506.
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