HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schlossmann, J. Articles by Neupert, W. Search for Related Content PubMed PubMed Citation Articles by Schlossmann, J. Articles by Neupert, W. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 269, Issue 16, 11893-11901, 04, 1994

Specific recognition of mitochondrial preproteins by the cytosolic domain of the import receptor MOM72

J Schlossmann, K Dietmeier, N Pfanner and W Neupert
Institut fur Physiologische Chemie, Universitat Munchen, Germany.

The import receptor MOM72 constitutes part of the protein translocation machinery of the outer mitochondrial membrane, the receptor-general insertion pore complex. The protein contains a membrane anchor at the NH2 terminus and a large cytosolic domain. In yeast and Neurospora crassa the cytosolic domain comprises about 570-580 amino acid residues. The cytosolic domain of yeast MOM72 was purified after expression in Escherichia coli as a homogeneous monomeric protein. It can recognize precursor proteins as demonstrated by its ability to compete for binding and import into the mitochondria and to physically interact with preproteins. A subset of preproteins including the ADP/ATP carrier and the phosphate carrier interact with very high affinity, precursors that are known to be targeted via MOM72. Thus, the cytosolic domain of MOM72 plays a critical function in the recognition of preproteins by directly binding to precursor proteins and thereby facilitating their targeting to mitochondria.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				T. Asai, T. Takahashi, M. Esaki, S.-i. Nishikawa, K. Ohtsuka, M. Nakai, and T. Endo Reinvestigation of the Requirement of Cytosolic ATP for Mitochondrial Protein Import J. Biol. Chem., May 7, 2004; 279(19): 19464 - 19470. [Abstract] [Full Text] [PDF]

				T. Waizenegger, T. Stan, W. Neupert, and D. Rapaport Signal-Anchor Domains of Proteins of the Outer Membrane of Mitochondria: STRUCTURAL AND FUNCTIONAL CHARACTERISTICS J. Biol. Chem., October 24, 2003; 278(43): 42064 - 42071. [Abstract] [Full Text] [PDF]

				T. Stan, J. Brix, J. Schneider-Mergener, N. Pfanner, W. Neupert, and D. Rapaport Mitochondrial Protein Import: Recognition of Internal Import Signals of BCS1 by the TOM Complex Mol. Cell. Biol., April 1, 2003; 23(7): 2239 - 2250. [Abstract] [Full Text] [PDF]

				S. Vial, H. Lu, S. Allen, P. Savory, D. Thornton, J. Sheehan, and K. Tokatlidis Assembly of Tim9 and Tim10 into a Functional Chaperone J. Biol. Chem., September 20, 2002; 277(39): 36100 - 36108. [Abstract] [Full Text] [PDF]

				U. Ahting, C. Thun, R. Hegerl, D. Typke, F. E. Nargang, W. Neupert, and S. Nussberger The TOM Core Complex: The General Protein Import Pore of the Outer Membrane of Mitochondria J. Cell Biol., November 29, 1999; 147(5): 959 - 968. [Abstract] [Full Text] [PDF]

				J. Brix, S. Rudiger, B. Bukau, J. Schneider-Mergener, and N. Pfanner Distribution of Binding Sequences for the Mitochondrial Import Receptors Tom20, Tom22, and Tom70 in a Presequence-carrying Preprotein and a Non-cleavable Preprotein J. Biol. Chem., June 4, 1999; 274(23): 16522 - 16530. [Abstract] [Full Text] [PDF]

				D. Rapaport, K.-P. Künkele, M. Dembowski, U. Ahting, F. E. Nargang, W. Neupert, and R. Lill Dynamics of the TOM Complex of Mitochondria during Binding and Translocation of Preproteins Mol. Cell. Biol., September 1, 1998; 18(9): 5256 - 5262. [Abstract] [Full Text]

				A. J. Davis, K. R. Ryan, and R. E. Jensen Tim23p Contains Separate and Distinct Signals for Targeting to Mitochondria and Insertion into the Inner Membrane Mol. Biol. Cell, September 1, 1998; 9(9): 2577 - 2593. [Abstract] [Full Text]

				K. R. Ryan, R. S. Leung, and R. E. Jensen Characterization of the Mitochondrial Inner Membrane Translocase Complex: the Tim23p Hydrophobic Domain Interacts with Tim17p but Not with Other Tim23p Molecules Mol. Cell. Biol., January 1, 1998; 18(1): 178 - 187. [Abstract] [Full Text]

				J. Brix, K. Dietmeier, and N. Pfanner Differential Recognition of Preproteins by the Purified Cytosolic Domains of the Mitochondrial Import Receptors Tom20, Tom22, and Tom70 J. Biol. Chem., August 15, 1997; 272(33): 20730 - 20735. [Abstract] [Full Text] [PDF]

				X. Xu and M. Colombini Self-catalyzed Insertion of Proteins into Phospholipid Membranes J. Biol. Chem., September 27, 1996; 271(39): 23675 - 23682. [Abstract] [Full Text] [PDF]

				J. W. Priest and S. L. Hajduk In Vitro Import of the Rieske Iron-Sulfur Protein by Trypanosome Mitochondria J. Biol. Chem., August 16, 1996; 271(33): 20060 - 20069. [Abstract] [Full Text] [PDF]

				J. Schlossmann, R. Lill, W. Neupert, and D. A. Court Tom71, a Novel Homologue of the Mitochondrial Preprotein Receptor Tom70 J. Biol. Chem., July 26, 1996; 271(30): 17890 - 17895. [Abstract] [Full Text] [PDF]

				J. Schlossmann and W. Neupert Assembly of the Preprotein Receptor MOM72/MAS70 into the Protein Import Complex of the Outer Membrane of Mitochondria J. Biol. Chem., November 10, 1995; 270(45): 27116 - 27121. [Abstract] [Full Text] [PDF]

				M. Dembowski, K.-P. Kunkele, F. E. Nargang, W. Neupert, and D. Rapaport Assembly of Tom6 and Tom7 into the TOM Core Complex of Neurospora crassa J. Biol. Chem., May 18, 2001; 276(21): 17679 - 17685. [Abstract] [Full Text] [PDF]