| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 269, Issue 26, 17495-17501, Jul, 1994
S Ohno, K Mizuno, Y Adachi, A Hata, Y Akita, K Akimoto, S Osada, S Hirai and K Suzuki
Rat fibroblast 3Y1 cells express at least three protein kinase C species,
conventional PKC alpha (cPKC alpha), novel PKC delta (nPKC delta), and
novel PKC epsilon (nPKC epsilon). The stimulation of quiescent 3Y1 cells by
serum (or epidermal growth factor (EGF) but not
12-O-tetradecanoylphorbol-13-acetate (TPA) results in the induction of DNA
synthesis. Upon stimulation by serum or EGF, endogenous PKC species showed
no indication of activation such as translocation or down- regulation,
whereas TPA or synthetic diacylglycerol caused activation of all these PKC
species when judged by these criteria. The only indication of activation
observed upon serum or EGF stimulation was an upward shift in the
electrophoretic mobility of nPKC delta. The phosphorylation levels of
endogenous PKC members determined by in vivo metabolic labeling experiments
revealed increased phosphorylation of both nPKC delta and nPKC epsilon, but
only a slight increase for cPKC alpha in response to serum or EGF. On the
other hand, TPA caused increased phosphorylation of all three PKC species.
Overexpression of these PKC members by introduction of the corresponding
cDNA expression plasmids resulted in the enhancement of the cell response
to TPA when monitored in terms of transcriptional activation through TPA-
or serum- responsive elements. Such enhancement in transcriptional
activation by overexpression of cPKC alpha, nPKC delta, or nPKC epsilon was
also observed in response to diacylglycerol, indicating that all these PKC
species are activated by diacylglycerol in cells. In contrast to these
nonphysiological stimuli, serum (or EGF) stimulation of 3Y1 cells that
overexpress the respective PKC members revealed a clear difference between
cPKC and nPKC, in that overexpression of nPKC delta or nPKC epsilon
resulted in a large increase in TPA- or serum-responsive element
activation, whereas the overexpression of cPKC alpha increased activation
only very slightly. These results indicate that the mitogenic stimulation
of quiescent 3Y1 cells results in selective activation of endogenous nPKC
members and that the modes of activation of cPKC and nPKC differ from each
other.
Activation of novel protein kinases C delta and C epsilon upon mitogenic stimulation of quiescent rat 3Y1 fibroblasts
Department of Molecular Biology, Yokohama City University School of Medicine, Japan.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Shimohata, H. Zhao, and G. K. Steinberg {epsilon}PKC May Contribute to the Protective Effect of Hypothermia in a Rat Focal Cerebral Ischemia Model Stroke, February 1, 2007; 38(2): 375 - 380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Barry and V. Giguere Epidermal Growth Factor-Induced Signaling in Breast Cancer Cells Results in Selective Target Gene Activation by Orphan Nuclear Receptor Estrogen-Related Receptor {alpha} Cancer Res., July 15, 2005; 65(14): 6120 - 6129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Saksena, R. K. Gill, S. Tyagi, W. A. Alrefai, Z. Sarwar, K. Ramaswamy, and P. K. Dudeja Involvement of c-Src and Protein Kinase C{delta} in the Inhibition of Cl-/OH- Exchange Activity in Caco-2 Cells by Serotonin J. Biol. Chem., March 25, 2005; 280(12): 11859 - 11868. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. Korzick, J. C. Hunter, M. K. McDowell, M. D. Delp, M. M. Tickerhoof, and L. D. Carson Chronic Exercise Improves Myocardial Inotropic Reserve Capacity Through {alpha}1-Adrenergic and Protein Kinase C-Dependent Effects in Senescent Rats J. Gerontol. A Biol. Sci. Med. Sci., November 1, 2004; 59(11): 1089 - 1098. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-Y. Park, H. Wu, C. E. Killoran, and B. A. Gilchrest The receptor for activated C-kinase-I (RACK-I) anchors activated PKC-{beta} on melanosomes J. Cell Sci., July 15, 2004; 117(16): 3659 - 3668. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Iwabu, K. Smith, F. D. Allen, D. A. Lauffenburger, and A. Wells Epidermal Growth Factor Induces Fibroblast Contractility and Motility via a Protein Kinase C {delta}-dependent Pathway J. Biol. Chem., April 9, 2004; 279(15): 14551 - 14560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Takenobu, A. Yamazaki, M. Hirata, T. Umata, and E. Mekada The Stress- and Inflammatory Cytokine-induced Ectodomain Shedding of Heparin-binding Epidermal Growth Factor-like Growth Factor Is Mediated by p38 MAPK, Distinct from the 12-O-Tetradecanoylphorbol-13-acetate- and Lysophosphatidic Acid-induced Signaling Cascades J. Biol. Chem., May 2, 2003; 278(19): 17255 - 17262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Pickett, N. Manning, Y. Akita, and A. Gutierrez-Hartmann Role of Specific Protein Kinase C Isozymes in Mediating Epidermal Growth Factor, Thyrotropin-Releasing Hormone, and Phorbol Ester Regulation of the Rat Prolactin Promoter in GH4/GH4C1 Pituitary Cells Mol. Endocrinol., December 1, 2002; 16(12): 2840 - 2852. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tsuru, H. Katagiri, T. Asano, T. Yamada, S. Ohno, T. Ogihara, and Y. Oka Role of PKC isoforms in glucose transport in 3T3-L1 adipocytes: insignificance of atypical PKC Am J Physiol Endocrinol Metab, August 1, 2002; 283(2): E338 - E345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Knauf, L. S. Ward, Y. E. Nikiforov, M. Nikiforova, E. Puxeddu, M. Medvedovic, T. Liron, D. Mochly-Rosen, and J. A. Fagin Isozyme-Specific Abnormalities of PKC in Thyroid Cancer: Evidence for Post-Transcriptional Changes in PKC Epsilon J. Clin. Endocrinol. Metab., May 1, 2002; 87(5): 2150 - 2159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Naucler, S. Grinstein, R. Sundler, and H. Tapper Signaling to localized degranulation in neutrophils adherent to immune complexes J. Leukoc. Biol., April 1, 2002; 71(4): 701 - 710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Reddig, N. E. Dreckschmidt, J. Zou, S. E. Bourguignon, T. D. Oberley, and A. K. Verma Transgenic Mice Overexpressing Protein Kinase C{{epsilon}} in Their Epidermis Exhibit Reduced Papilloma Burden but Enhanced Carcinoma Formation after Tumor Promotion Cancer Res., February 1, 2000; 60(3): 595 - 602. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Izumi, T. Hirose, Y. Tamai, S.-i. Hirai, Y. Nagashima, T. Fujimoto, Y. Tabuse, K. J. Kemphues, and S. Ohno An Atypical PKC Directly Associates and Colocalizes at the Epithelial Tight Junction with ASIP, a Mammalian Homologue of Caenorhabditis elegans Polarity Protein PAR-3 J. Cell Biol., October 5, 1998; 143(1): 95 - 106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ohmori, Y. Shirai, N. Sakai, M. Fujii, H. Konishi, U. Kikkawa, and N. Saito Three Distinct Mechanisms for Translocation and Activation of the delta Subspecies of Protein Kinase C Mol. Cell. Biol., September 1, 1998; 18(9): 5263 - 5271. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Izumi, S.-i. Hirai, Y. Tamai, A. Fujise-Matsuoka, Y. Nishimura, and S. Ohno A Protein Kinase Cdelta -binding Protein SRBC Whose Expression Is Induced by Serum Starvation J. Biol. Chem., March 14, 1997; 272(11): 7381 - 7389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. R. Smith, P. L. Jones, J. M. Boss, and A. H. Merrill Jr. Changing J774A.1 Cells to New Medium Perturbs Multiple Signaling Pathways, Including the Modulation of Protein Kinase C by Endogenous Sphingoid Bases J. Biol. Chem., February 28, 1997; 272(9): 5640 - 5646. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gatti and P. J. Robinson Unique Phosphorylation of Protein Kinase C-alpha in PC12 Cells Induces Resistance to Translocation and Down-regulation J. Biol. Chem., December 6, 1996; 271(49): 31718 - 31722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Reifel-Miller, D. M. Conarty, K. M. Valasek, P. W. Iversen, D. J. Burns, and K. A. Birch Protein Kinase C Isozymes Differentially Regulate Promoters Containing PEA-3/12-O-Tetradecanoylphorbol-13-acetate Response Element Motifs J. Biol. Chem., August 30, 1996; 271(35): 21666 - 21671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N.-Y. Shih and G. Floyd-Smith Protein Kinase C-delta mRNA Is Down-regulated Transcriptionally and Post-transcriptionally by 12-O-Tetradecanoylphorbol-13-acetate J. Biol. Chem., July 5, 1996; 271(27): 16040 - 16046. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Ettinger, R. W. Lauener, and V. Duronio Protein Kinase C delta Specifically Associates with Phosphatidylinositol 3-Kinase Following Cytokine Stimulation J. Biol. Chem., June 14, 1996; 271(24): 14514 - 14518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Eto, Y. Akita, T. C. Saido, K. Suzuki, and S. Kawashima The Role of the Calpain-Calpastatin System in Thyrotropin-releasing Hormone-induced Selective Down-regulation of a Protein Kinase C Isozyme, nPKC[IMAGE], in Rat Pituitary GH(4)C(1) Cells J. Biol. Chem., October 20, 1995; 270(42): 25115 - 25120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bj, M. T. Diaz-Meco, J. Moscat, and T. Johansen Evidence for a Bifurcation of the Mitogenic Signaling Pathway Activated by Ras and Phosphatidylcholine-hydrolyzing Phospholipase C J. Biol. Chem., September 8, 1995; 270(36): 21299 - 21306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Umata, M. Hirata, T. Takahashi, F. Ryu, S. Shida, Y. Takahashi, M. Tsuneoka, Y. Miura, M. Masuda, Y. Horiguchi, et al. A Dual Signaling Cascade That Regulates the Ectodomain Shedding of Heparin-binding Epidermal Growth Factor-like Growth Factor J. Biol. Chem., August 3, 2001; 276(32): 30475 - 30482. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
