HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Balvay, L. Articles by Fiszman, M. Y. Search for Related Content PubMed PubMed Citation Articles by Balvay, L. Articles by Fiszman, M. Y. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 269, Issue 31, 19675-19678, 08, 1994

Splicing of the alternative exons of the chicken, rat, and Xenopus beta tropomyosin transcripts requires class-specific elements

L Balvay, AM Pret, D Libri, DM Helfman and MY Fiszman
Departement de Biologie Moleculaire, Institut Pasteur, Paris, France.

The diversity of protein isoforms is often generated from single genes by alternative splicing of the primary transcript. Using transfection of beta tropomyosin minigene constructs into homologous and heterologous cell systems, we show that there are differences, among higher vertebrates, in the components of the splicing machinery which control the conserved regulated splicing pattern of two mutually exclusive exons (6A and 6B) present in this gene. These experiments demonstrate that genes which give rise to alternative transcripts may require an appropriate combination of splicing factors which are species-specific, or at least restricted to the same taxonomic subgroup (class). An important practical implication is that the splicing of these genes may be deregulated in heterologous systems in vitro and in vivo, i.e. in transgenic animals.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Laverdiere, J. Beaudoin, and A. Lavigueur Species-specific regulation of alternative splicing in the C-terminal region of the p53 tumor suppressor gene Nucleic Acids Res., March 15, 2000; 28(6): 1489 - 1497. [Abstract] [Full Text] [PDF]

				S Russo, D Tomatis, G Collo, G Tarone, and F Tato Myogenic conversion of NIH3T3 cells by exogenous MyoD family members: dissociation of terminal differentiation from myotube formation J. Cell Sci., January 3, 1998; 111(6): 691 - 700. [Abstract] [PDF]

				A.-M. Pret and M. Y. Fiszman Sequence Divergence Associated with Species-specific Splicing of the Nonmuscle beta-Tropomyosin Alternative Exon J. Biol. Chem., May 10, 1996; 271(19): 11511 - 11517. [Abstract] [Full Text] [PDF]