| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 269, Issue 31, 19859-19867, Aug, 1994
PD Kassner, S Kawaguchi and ME Hemler
Previously, we found that deletion of the integrin alpha 4 and alpha 2
subunit cytoplasmic domains, just after the conserved GFFKR motif, causes a
loss of adhesive activity mediated by VLA-4 or VLA-2, respectively
(Kassner, P.D., and Hemler, M. E. (1993) J. Exp. Med. 178, 649-60;
Kawaguchi, S., and Hemler, M. E. (1993) J. Biol. Chem. 268, 16279-12685).
Here, we show for alpha 4 and alpha 2 chains (expressed in MIP101 and
Chinese hamster ovary cells) that adding only 3-4 amino acids after the
GFFKR motif restores substantial adhesive activity and that 5-7 amino acids
confers maximal adhesive activity (to VCAM-1, CS1 peptide, or collagen,
respectively). Point mutations within the most critical 5 alpha 4 residues
had no effect on alpha 4 adhesive activity, nor did exchange of the alpha 4
tail with that of alpha 2. Thus, only a short and relatively nonspecific
stretch of alpha chain cytoplasmic domain amino acids may be required to
achieve maximal integrin adhesive activity. Also, comprehensive divalent
cation titration assays revealed (i) that deletion of alpha chain
cytoplasmic domains caused a marked decrease in the efficiency of divalent
cation utilization during cell adhesion assays and (ii) that cytoplasmic
domain deletion effects could be either suppressed or accentuated depending
on the type and amount of divalent cation and the cellular environment
utilized. Notably, integrin alpha chain tail deletions did not appear to
alter the intrinsic ability to interact with ligand because deletion
effects were minimal in the presence of metabolic energy inhibitors and
were absent during cell-free ligand binding assays.
Minimum alpha chain cytoplasmic tail sequence needed to support integrin-mediated adhesion
Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. E. Goldfinger, E. Tzima, R. Stockton, W. B. Kiosses, K. Kinbara, E. Tkachenko, E. Gutierrez, A. Groisman, P. Nguyen, S. Chien, et al. Localized {alpha}4 Integrin Phosphorylation Directs Shear Stress-Induced Endothelial Cell Alignment Circ. Res., July 18, 2008; 103(2): 177 - 185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Humphries, N. R. Schofield, Z. Mostafavi-Pour, L. J. Green, A. N. Garratt, A. P. Mould, and M. J. Humphries Dual Functionality of the Anti-{beta}1 Integrin Antibody, 12G10, Exemplifies Agonistic Signalling from the Ligand Binding Pocket of Integrin Adhesion Receptors J. Biol. Chem., March 18, 2005; 280(11): 10234 - 10243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Calderwood Integrin activation J. Cell Sci., March 1, 2004; 117(5): 657 - 666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Na, M. Marsden, and D. W. DeSimone Differential regulation of cell adhesive functions by integrin {alpha} subunit cytoplasmic tails in vivo J. Cell Sci., June 1, 2003; 116(11): 2333 - 2343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tohyama, K. Katagiri, R. Pardi, C. Lu, T. A. Springer, and T. Kinashi The Critical Cytoplasmic Regions of the {alpha}L/{beta}2 Integrin in Rap1-induced Adhesion and Migration Mol. Biol. Cell, June 1, 2003; 14(6): 2570 - 2582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. T. de Bruyn, S. Rangarajan, K. A. Reedquist, C. G. Figdor, and J. L. Bos The Small GTPase Rap1 Is Required for Mn2+- and Antibody-induced LFA-1- and VLA-4-mediated Cell Adhesion J. Biol. Chem., August 9, 2002; 277(33): 29468 - 29476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. K. Cherry, K. S. C. Weber, and L. B. Klickstein A Dominant Jurkat T Cell Mutation That Inhibits LFA-1-Mediated Cell Adhesion Is Associated with Increased Cell Growth J. Immunol., December 1, 2001; 167(11): 6171 - 6179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. F. Kucik, T. E. O'Toole, A. Zheleznyak, D. K. Busettini, and E. J. Brown Activation-enhanced {alpha}IIb{beta}3-Integrin-Cytoskeleton Interactions Outside of Focal Contacts Require the {alpha}-Subunit Mol. Biol. Cell, May 1, 2001; 12(5): 1509 - 1518. [Abstract] [Full Text]
|
|
|
|
|
|
A. Armulik, I. Nilsson, G. von Heijne, and S. Johansson Determination of the Border between the Transmembrane and Cytoplasmic Domains of Human Integrin Subunits J. Biol. Chem., December 24, 1999; 274(52): 37030 - 37034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ivaska, H. Reunanen, J. Westermarck, L. Koivisto, V.-M. Kahari, and J. Heino Integrin {alpha}2{beta}1 Mediates Isoform-specific Activation of p38 and Upregulation of Collagen Gene Transcription by a Mechanism Involving the {alpha}2 Cytoplasmic Tail J. Cell Biol., October 18, 1999; 147(2): 401 - 416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bittner, U. Gosslar, A. Luz, and B. Holzmann Sequence Motifs in the Integrin {alpha}4 Cytoplasmic Tail Required for Regulation of In Vivo Expansion of Murine Lymphoma Cells J. Immunol., December 1, 1998; 161(11): 5978 - 5986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Peyruchaud, A.T. Nurden, S. Milet, L. Macchi, A. Pannochia, P.F. Bray, N. Kieffer, and F. Bourre R to Q Amino Acid Substitution in the GFFKR Sequence of the Cytoplasmic Domain of the Integrin alpha IIb Subunit in a Patient With a Glanzmann's Thrombasthenia-Like Syndrome Blood, December 1, 1998; 92(11): 4178 - 4187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Martin-Bermudo, O. M. Dunin-Borkowski, and N. H. Brown Modulation of Integrin Activity is Vital for Morphogenesis J. Cell Biol., May 18, 1998; 141(4): 1073 - 1081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Stewart, A. McDowall, and N. Hogg LFA-1-mediated Adhesion Is Regulated by Cytoskeletal Restraint and by a Ca2+-dependent Protease, Calpain J. Cell Biol., February 9, 1998; 140(3): 699 - 707. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Yauch, D. P. Felsenfeld, S.-K. Kraeft, L. B. Chen, M. P. Sheetz, and M. E. Hemler Mutational Evidence for Control of Cell Adhesion Through Integrin Diffusion/Clustering, Independent of Ligand Binding J. Exp. Med., October 20, 1997; 186(8): 1347 - 1355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lyman, A. Gilmore, K. Burridge, S. Gidwitz, and G. C. White II Integrin-mediated Activation of Focal Adhesion Kinase Is Independent of Focal Adhesion Formation or Integrin Activation. STUDIES WITH ACTIVATED AND INHIBITORY beta 3 CYTOPLASMIC DOMAIN MUTANTS J. Biol. Chem., September 5, 1997; 272(36): 22538 - 22547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Dickeson, J. J. Walsh, and S. A. Santoro Contributions of the I and EF Hand Domains to the Divalent Cation-dependent Collagen Binding Activity of the alpha 2beta 1 Integrin J. Biol. Chem., March 21, 1997; 272(12): 7661 - 7668. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Loh, W. Qi, G. Vilaire, and J. S. Bennett Effect of Cytoplasmic Domain Mutations on the Agonist-stimulated Ligand Binding Activity of the Platelet Integrin alpha IIbbeta 3 J. Biol. Chem., November 22, 1996; 271(47): 30233 - 30241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Haas and E. F. Plow The Cytoplasmic Domain of alpha[IMAGE]beta(3) J. Biol. Chem., March 15, 1996; 271(11): 6017 - 6026. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bazzoni, D.-T. Shih, C. A. Buck, and M. E. Hemler Monoclonal Antibody 9EG7 Defines a Novel beta(1) Integrin Epitope Induced by Soluble Ligand and Manganese, but Inhibited by Calcium J. Biol. Chem., October 27, 1995; 270(43): 25570 - 25577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. E. Hughes, T. E. O'Toole, J. Ylänne, S. J. Shattil, and M. H. Ginsberg The Conserved Membrane-proximal Region of an Integrin Cytoplasmic Domain Specifies Ligand Binding Affinity J. Biol. Chem., May 26, 1995; 270(21): 12411 - 12417. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Clark and J. Brugge Integrins and signal transduction pathways: the road taken Science, April 14, 1995; 268(5208): 233 - 239. [Abstract] [PDF]
|
|
|
|
|
|
J. Weitzman, A Chen, and M. Hemler Investigation of the role of beta 1 integrins in cell-cell adhesion J. Cell Sci., January 11, 1995; 108(11): 3635 - 3644. [Abstract] [PDF]
|
|
|
|
|
|
V. Wixler, D. Geerts, E. Laplantine, D. Westhoff, N. Smyth, M. Aumailley, A. Sonnenberg, and M. Paulsson The LIM-only Protein DRAL/FHL2 Binds to the Cytoplasmic Domain of Several alpha and beta Integrin Chains and Is Recruited to Adhesion Complexes J. Biol. Chem., October 20, 2000; 275(43): 33669 - 33678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Klekotka, S. A. Santoro, H. Wang, and M. M. Zutter Specific Residues within the alpha 2 Integrin Subunit Cytoplasmic Domain Regulate Migration and Cell Cycle Progression via Distinct MAPK Pathways J. Biol. Chem., August 17, 2001; 276(34): 32353 - 32361. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
