| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 269, Issue 31, 19897-19903, 08, 1994
S Hara, A Miyata, C Yokoyama, H Inoue, R Brugger, F Lottspeich, V Ullrich and T Tanabe
Prostacyclin synthase catalyzes the conversion of prostaglandin H2 to
prostacyclin, which is a powerful vasodilator and the most potent natural
occurring inhibitor of platelet aggregation. In the present study, we
determined the amino acid sequence of bovine prostacyclin synthase by
combined protein chemical and molecular cloning techniques. The enzyme was
purified and characterized from bovine aorta microsomes, and the partial
amino acid sequences were determined with the native enzyme and
endoproteinase Lys-C-cleaved peptides. Using primers synthesized according
to the amino acid sequences, cDNA coding for prostacyclin synthase was
amplified by polymerase chain reaction with bovine endothelial cell
poly(A)+ RNA and cloned into pBluescript II. Nucleotide sequence analyses
of the cloned cDNA inserts revealed that cDNA for this enzyme contained a
1500-base pair open reading frame coding for a 500-amino acid polypeptide
with a M(r) of 56,628. COS-7 cells transfected with an expression plasmid
harboring this cDNA clone expressed prostacyclin synthase activity. The
primary structure of the enzyme showed structural characteristics of
cytochrome P450 and exhibited a 32% identity to that of human cholesterol 7
alpha- hydroxylase. However, the identity between the amino acid sequences
of bovine prostacyclin synthase and human thromboxane synthase was only
16%, and no P450 showed an identity higher than 40%, suggesting that
prostacyclin synthase represents a new family in the P450 superfamily. RNA
blot analysis indicated that the mRNA for prostacyclin synthase from bovine
endothelial cells showed a size of approximately 2.7 kilobases and that the
mRNA level increased about 3-fold by treatment of tumor necrosis
factor-alpha.
Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells
Department of Pharmacology, National Cardiovascular Center Research Institute, Osaka, Japan.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. He, T. Lu, L. V. d'Uscio, C.-F. Lam, H.-C. Lee, and Z. S. Katusic Angiogenic Function of Prostacyclin Biosynthesis in Human Endothelial Progenitor Cells Circ. Res., July 3, 2008; 103(1): 80 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-C. Li, C.-W. Chiang, H.-C. Yeh, P.-Y. Hsu, F. G. Whitby, L.-H. Wang, and N.-L. Chan Structures of Prostacyclin Synthase and Its Complexes with Substrate Analog and Inhibitor Reveal a Ligand-specific Heme Conformation Change J. Biol. Chem., February 1, 2008; 283(5): 2917 - 2926. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Wang, E. Zolty, S. Falk, S. Summer, R. Stearman, M. Geraci, and R. Schrier Prostacyclin in endotoxemia-induced acute kidney injury: cyclooxygenase inhibition and renal prostacyclin synthase transgenic mice Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1131 - F1136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. F. Rowley, C. L. Vogan, G. W. Taylor, and A. S. Clare Prostaglandins in non-insectan invertebrates: recent insights and unsolved problems J. Exp. Biol., January 1, 2005; 208(1): 3 - 14. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Kim, Y. Zou, S. Yoon, J. H. Lee, Y. K. Kim, B. P. Yu, and H. Y. Chung Vascular Aging: Molecular Modulation of the Prostanoid Cascade by Calorie Restriction J. Gerontol. A Biol. Sci. Med. Sci., September 1, 2004; 59(9): B876 - B885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Whitsett, C. J. Bachurski, K. C. Barnes, P. A. Bunn Jr., L. M. Case, D. N. Cook, D. Crooks, M. W. Duncan, L. Dwyer-Nield, R. C. Elston, et al. Functional Genomics of Lung Disease Am. J. Respir. Cell Mol. Biol., August 1, 2004; 31(2/S1): S1 - S81. [Full Text] [PDF]
|
|
|
|
 |
|
 T. D. WARNER and J. A. MITCHELL Cyclooxygenases: new forms, new inhibitors, and lessons from the clinic FASEB J, May 1, 2004; 18(7): 790 - 804. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wada, C. Yokoyama, T. Hatae, M. Shimonishi, M. Nakamura, Y. Imai, V. Ullrich, and T. Tanabe Purification and Characterization of Recombinant Human Prostacyclin Synthase J. Biochem., April 1, 2004; 135(4): 455 - 463. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Cosentino, M. Eto, P. De Paolis, B. van der Loo, M. Bachschmid, V. Ullrich, A. Kouroedov, C. Delli Gatti, H. Joch, M. Volpe, et al. High Glucose Causes Upregulation of Cyclooxygenase-2 and Alters Prostanoid Profile in Human Endothelial Cells: Role of Protein Kinase C and Reactive Oxygen Species Circulation, February 25, 2003; 107(7): 1017 - 1023. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Shinmura, M. Nagai, K. Tamaki, M. Tani, and R. Bolli COX-2-derived prostacyclin mediates opioid-induced late phase of preconditioning in isolated rat hearts Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2534 - H2543. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Korita, N. Sagawa, H. Itoh, S. Yura, M. Yoshida, K. Kakui, M. Takemura, C. Yokoyama, T. Tanabe, and S. Fujii Cyclic Mechanical Stretch Augments Prostacyclin Production in Cultured Human Uterine Myometrial Cells from Pregnant Women: Possible Involvement of Up-Regulation of Prostacyclin Synthase Expression J. Clin. Endocrinol. Metab., November 1, 2002; 87(11): 5209 - 5219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Yokoyama, T. Yabuki, M. Shimonishi, M. Wada, T. Hatae, S. Ohkawara, J. Takeda, T. Kinoshita, M. Okabe, and T. Tanabe Prostacyclin-Deficient Mice Develop Ischemic Renal Disorders, Including Nephrosclerosis and Renal Infarction Circulation, October 29, 2002; 106(18): 2397 - 2403. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Suhara, Y. Sawa, N. Fukushima, K. Kagisaki, C. Yokoyama, T. Tanabe, S. Ohtake, and H. Matsuda Gene transfer of human prostacyclin synthase into the liver is effective for the treatment of pulmonary hypertension in rats J. Thorac. Cardiovasc. Surg., May 1, 2002; 123(5): 855 - 861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hatae, M. Wada, C. Yokoyama, M. Shimonishi, and T. Tanabe Prostacyclin-dependent Apoptosis Mediated by PPARdelta J. Biol. Chem., November 30, 2001; 276(49): 46260 - 46267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. Wright, D. Abran, M. Bhattacharya, X. Hou, S. G. Bernier, A. Bouayad, J.-C. Fouron, A. Vazquez-Tello, M. H. Beauchamp, R. I. Clyman, et al. Prostanoid receptors: ontogeny and implications in vascular physiology Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2001; 281(5): R1343 - R1360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Soler, M. Camacho, J.-R. Escudero, M. A. Iniguez, and L. Vila Human Vascular Smooth Muscle Cells but Not Endothelial Cells Express Prostaglandin E Synthase Circ. Res., September 15, 2000; 87(6): 504 - 507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Y. Liou, S.-K. Shyue, M.-J. Tsai, C.-L. Chung, K.-Y. Chu, and K. K. Wu Colocalization of Prostacyclin Synthase with Prostaglandin H Synthase-1 (PGHS-1) but Not Phorbol Ester-induced PGHS-2 in Cultured Endothelial Cells J. Biol. Chem., May 12, 2000; 275(20): 15314 - 15320. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Siegle, T. Klein, M.-H. Zou, P. Fritz, and M. Kömhoff Distribution and Cellular Localization of Prostacyclin Synthase in Human Brain J. Histochem. Cytochem., May 1, 2000; 48(5): 631 - 642. [Abstract] [Full Text]
|
|
|
|
|
|
N. Iwai, T. Katsuya, K. Ishikawa, T. Mannami, J. Ogata, J. Higaki, T. Ogihara, T. Tanabe, and S. Baba Human Prostacyclin Synthase Gene and Hypertension : The Suita Study Circulation, November 30, 1999; 100(22): 2231 - 2236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Todaka, C. Yokoyama, H. Yanamoto, N. Hashimoto, I. Nagata, T. Tsukahara, S. Hara, T. Hatae, R. Morishita, M. Aoki, et al. Gene Transfer of Human Prostacyclin Synthase Prevents Neointimal Formation After Carotid Balloon Injury in Rats • Editorial Comment Stroke, February 1, 1999; 30(2): 419 - 426. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Klein, V. Ullrich, J. Pfeilschifter, and R. Nüsing On the Induction of Cyclooxygenase-2, Inducible Nitric Oxide Synthase and Soluble Phospholipase A2 in Rat Mesangial Cells by a Nonsteroidal Anti-Inflammatory Drug: The Role of Cyclic AMP Mol. Pharmacol., March 1, 1998; 53(3): 385 - 391. [Abstract] [Full Text]
|
|
|
|
|
|
S.-K. Shyue, K.-H. Ruan, L.-H. Wang, and K. K. Wu Prostacyclin Synthase Active Sites. IDENTIFICATION BY MOLECULAR MODELING-GUIDED SITE-DIRECTED MUTAGENESIS J. Biol. Chem., February 7, 1997; 272(6): 3657 - 3662. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Eggertsen, M. Olin, U. Andersson, H. Ishida, S. Kubota, U. Hellman, K.-I. Okuda, and I. Bjorkhem Molecular Cloning and Expression of Rabbit Sterol 12alpha -Hydroxylase J. Biol. Chem., December 13, 1996; 271(50): 32269 - 32275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-H. Wang, A.-L. Tsai, and P.-Y. Hsu Substrate Binding Is the Rate-limiting Step in Thromboxane Synthase Catalysis J. Biol. Chem., April 27, 2001; 276(18): 14737 - 14743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ueno, M. Murakami, T. Tanioka, K. Fujimori, T. Tanabe, Y. Urade, and I. Kudo Coupling between Cyclooxygenase, Terminal Prostanoid Synthase, and Phospholipase A2 J. Biol. Chem., September 7, 2001; 276(37): 34918 - 34927. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
