| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 269, Issue 4, 2369-2372, Jan, 1994
Y Zheng, R Cerione and A Bender
Bud emergence in Saccharomyces cerevisiae involves cell cycle-regulated
reorganizations of cortical cytoskeletal elements and requires the action
of the Rho (Ras homologous)-type GTPase Cdc42. As a first step toward
understanding how these cytoskeletal rearrangements are controlled, we have
sought to identify those proteins that regulate the binding and hydrolysis
of GTP by Cdc42. Here we report that the product of the CDC24 gene, which
is required for proper bud-site selection and bud emergence, can stimulate
the exchange of GTP for GDP on Cdc42. Combined with previously reported
genetic data, this finding suggests that the processes of bud-site
selection (which requires the action of a Ras-type GTPase) and bud-site
assembly might be coordinated through an activator of a Rho-type GTPase. We
also report the identification of a new gene, BEM3, that is a multicopy
suppressor of the temperature- sensitive lethality caused by mutations in
the bud emergence gene BEM2. Bem3 and Bem2 both contain a Rho
GTPase-activating protein homology domain, but only Bem3 is able to
stimulate the hydrolysis of GTP on Cdc42. These studies demonstrate that
Cdc24 and Bem3 have GDP-release factor activity and GTPase-activating
protein activity, respectively, toward the essential bud-site assembly
GTPase Cdc42.
Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bem3
Department of Pharmacology, Cornell University, Ithaca, New York 14850.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Mionnet, S. Bogliolo, and R. A. Arkowitz Oligomerization Regulates the Localization of Cdc24, the Cdc42 Activator in Saccharomyces cerevisiae J. Biol. Chem., June 20, 2008; 283(25): 17515 - 17530. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Tong, X.-D. Gao, A. S. Howell, I. Bose, D. J. Lew, and E. Bi Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein mediated zone of inhibition J. Cell Biol., December 31, 2007; 179(7): 1375 - 1384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-O. Park and E. Bi Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond Microbiol. Mol. Biol. Rev., March 1, 2007; 71(1): 48 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Court and P. Sudbery Regulation of Cdc42 GTPase Activity in the Formation of Hyphae in Candida albicans Mol. Biol. Cell, January 1, 2007; 18(1): 265 - 281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Iwase, J. Luo, S. Nagaraj, M. Longtine, H. B. Kim, B. K. Haarer, C. Caruso, Z. Tong, J. R. Pringle, and E. Bi Role of a Cdc42p Effector Pathway in Recruitment of the Yeast Septins to the Presumptive Bud Site Mol. Biol. Cell, March 1, 2006; 17(3): 1110 - 1125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. R. Parrish, C. J. Stefan, and S. D. Emr PtdIns(3)P accumulation in triple lipid-phosphatase-deletion mutants triggers lethal hyperactivation of the Rho1p/Pkc1p cell-integrity MAP kinase pathway J. Cell Sci., December 1, 2005; 118(23): 5589 - 5601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Tcheperegine, X.-D. Gao, and E. Bi Regulation of Cell Polarity by Interactions of Msb3 and Msb4 with Cdc42 and Polarisome Components Mol. Cell. Biol., October 1, 2005; 25(19): 8567 - 8580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, W. Chen, D. M. Simpson, and E. A. Elion Cdc24 Regulates Nuclear Shuttling and Recruitment of the Ste5 Scaffold to a Heterotrimeric G Protein in Saccharomyces cerevisiae J. Biol. Chem., April 1, 2005; 280(13): 13084 - 13096. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-D. Gao, J. P. Caviston, S. E. Tcheperegine, and E. Bi Pxl1p, a Paxillin-like Protein in Saccharomyces cerevisiae, May Coordinate Cdc42p and Rho1p Functions during Polarized Growth Mol. Biol. Cell, September 1, 2004; 15(9): 3977 - 3985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Wild, J. W. Yu, M. A. Lemmon, and K. J. Blumer The p21-activated Protein Kinase-related Kinase Cla4 Is a Coincidence Detector of Signaling by Cdc42 and Phosphatidylinositol 4-Phosphate J. Biol. Chem., April 23, 2004; 279(17): 17101 - 17110. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Mackin, T. J. Sousou, and S. E. Erdman The PXL1 Gene of Saccharomyces cerevisiae Encodes a Paxillin-like Protein Functioning in Polarized Cell Growth Mol. Biol. Cell, April 1, 2004; 15(4): 1904 - 1917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bidlingmaier and M. Snyder Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators J. Cell Biol., January 19, 2004; 164(2): 207 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hirota, K. Tanaka, K. Ohta, and M. Yamamoto Gef1p and Scd1p, the Two GDP-GTP Exchange Factors for Cdc42p, Form a Ring Structure that Shrinks during Cytokinesis in Schizosaccharomyces pombe Mol. Biol. Cell, September 1, 2003; 14(9): 3617 - 3627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Misu, K. Fujimura-Kamada, T. Ueda, A. Nakano, H. Katoh, and K. Tanaka Cdc50p, a Conserved Endosomal Membrane Protein, Controls Polarized Growth in Saccharomyces cerevisiae Mol. Biol. Cell, February 1, 2003; 14(2): 730 - 747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cheng, K. L. Rossman, G. M. Mahon, D. K. Worthylake, M. Korus, J. Sondek, and I. P. Whitehead RhoGEF Specificity Mutants Implicate RhoA as a Target for Dbs Transforming Activity Mol. Cell. Biol., October 1, 2002; 22(19): 6895 - 6905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Caviston, S. E. Tcheperegine, and E. Bi Singularity in budding: A role for the evolutionarily conserved small GTPase Cdc42p PNAS, September 17, 2002; 99(19): 12185 - 12190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Rodriguez-Pachon, H. Martin, G. North, R. Rotger, C. Nombela, and M. Molina A Novel Connection between the Yeast Cdc42 GTPase and the Slt2-mediated Cell Integrity Pathway Identified through the Effect of Secreted Salmonella GTPase Modulators J. Biol. Chem., July 19, 2002; 277(30): 27094 - 27102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. R. Smith, S. A. Givan, P. Cullen, and G. F. Sprague Jr. GTPase-Activating Proteins for Cdc42 Eukaryot. Cell, June 1, 2002; 1(3): 469 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Schmelzle, S. B. Helliwell, and M. N. Hall Yeast Protein Kinases and the RHO1 Exchange Factor TUS1 Are Novel Components of the Cell Integrity Pathway in Yeast Mol. Cell. Biol., March 1, 2002; 22(5): 1329 - 1339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Seeley, M. Kato, N. Margolis, W. Wickner, and G. Eitzen Genomic Analysis of Homotypic Vacuole Fusion Mol. Biol. Cell, March 1, 2002; 13(3): 782 - 794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Segev Ypt/Rab GTPases: Regulators of Protein Trafficking Sci. Signal., September 18, 2001; 2001(100): re11 - re11. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Drees, B. Sundin, E. Brazeau, J. P. Caviston, G.-C. Chen, W. Guo, K. G. Kozminski, M. W. Lau, J. J. Moskow, A. Tong, et al. A protein interaction map for cell polarity development J. Cell Biol., August 6, 2001; 154(3): 549 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Zhu, B. Debreceni, F. Bi, and Y. Zheng Oligomerization of DH Domain Is Essential for Dbl-Induced Transformation Mol. Cell. Biol., January 15, 2001; 21(2): 425 - 437. [Abstract] [Full Text]
|
|
|
|
|
|
H.-U. Mösch, T. Köhler, and G. H. Braus Different Domains of the Essential GTPase Cdc42p Required for Growth and Development of Saccharomyces cerevisiae Mol. Cell. Biol., January 1, 2001; 21(1): 235 - 248. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. P. Finger and P. Novick Synthetic Interactions of the Post-Golgi sec Mutations of Saccharomyces cerevisiae Genetics, November 1, 2000; 156(3): 943 - 951. [Abstract] [Full Text]
|
|
|
|
|
|
C. Sette, C. J. Inouye, S. L. Stroschein, P. J. Iaquinta, and J. Thorner Mutational Analysis Suggests That Activation of the Yeast Pheromone Response Mitogen-activated Protein Kinase Pathway Involves Conformational Changes in the Ste5 Scaffold Protein Mol. Biol. Cell, November 1, 2000; 11(11): 4033 - 4049. [Abstract] [Full Text]
|
|
|
|
|
|
E. Bi, J. B. Chiavetta, H. Chen, G.-C. Chen, C. S. M. Chan, and J. R. Pringle Identification of Novel, Evolutionarily Conserved Cdc42p-interacting Proteins and of Redundant Pathways Linking Cdc24p and Cdc42p to Actin Polarization in Yeast Mol. Biol. Cell, February 1, 2000; 11(2): 773 - 793. [Abstract] [Full Text]
|
|
|
|
|
|
J Wendland and P Philippsen Determination of cell polarity in germinated spores and hyphal tips of the filamentous ascomycete Ashbya gossypii requires a rhoGAP homolog J. Cell Sci., January 5, 2000; 113(9): 1611 - 1621. [Abstract] [PDF]
|
|
|
|
|
|
D Pruyne and A Bretscher Polarization of cell growth in yeast. I. Establishment and maintenance of polarity states J. Cell Sci., January 2, 2000; 113(3): 365 - 375. [Abstract] [PDF]
|
|
|
|
|
|
K. G. Kozminski, A. J. Chen, A. A. Rodal, and D. G. Drubin Functions and Functional Domains of the GTPase Cdc42p Mol. Biol. Cell, January 1, 2000; 11(1): 339 - 354. [Abstract] [Full Text]
|
|
|
|
|
|
S. Jones, G. Jedd, R. A. Kahn, A. Franzusoff, F. Bartolini, and N. Segev Genetic Interactions in Yeast Between Ypt GTPases and Arf Guanine Nucleotide Exchangers Genetics, August 1, 1999; 152(4): 1543 - 1556. [Abstract] [Full Text]
|
|
|
|
|
|
T. J. Richman, M. M. Sawyer, and D. I. Johnson The Cdc42p GTPase Is Involved in a G2/M Morphogenetic Checkpoint Regulating the Apical-Isotropic Switch and Nuclear Division in Yeast J. Biol. Chem., June 11, 1999; 274(24): 16861 - 16870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. I. Johnson Cdc42: An Essential Rho-Type GTPase Controlling Eukaryotic Cell Polarity Microbiol. Mol. Biol. Rev., March 1, 1999; 63(1): 54 - 105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Zeng and M. Cai Regulation of the Actin Cytoskeleton Organization in Yeast by a Novel Serine/Threonine Kinase Prk1p J. Cell Biol., January 11, 1999; 144(1): 71 - 82. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. W. Pruyne, D. H. Schott, and A. Bretscher Tropomyosin-containing Actin Cables Direct the Myo2p-dependent Polarized Delivery of Secretory Vesicles in Budding Yeast J. Cell Biol., December 28, 1998; 143(7): 1931 - 1945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Gustin, J. Albertyn, M. Alexander, and K. Davenport MAP Kinase Pathways in the Yeast Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., December 1, 1998; 62(4): 1264 - 1300. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Butty, P. M. Pryciak, L. S. Huang, I. Herskowitz, and M. Peter The Role of Far1p in Linking the Heterotrimeric G Protein to Polarity Establishment Proteins During Yeast Mating Science, November 20, 1998; 282(5393): 1511 - 1516. [Abstract] [Full Text]
|
|
|
|
|
|
N. Lamarche-Vane and A. Hall CdGAP, a Novel Proline-rich GTPase-activating Protein for Cdc42 and Rac J. Biol. Chem., October 30, 1998; 273(44): 29172 - 29177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kim and J. P. Hirsch A Nucleolar Protein That Affects Mating Efficiency in Saccharomyces cerevisiae by Altering the Morphological Response to Pheromone Genetics, June 1, 1998; 149(2): 795 - 805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. W. M. Oehlen and F. R. Cross The Role of Cdc42 in Signal Transduction and Mating of the Budding Yeast Saccharomyces cerevisiae J. Biol. Chem., April 10, 1998; 273(15): 8556 - 8559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Häcker and N. Perrimon DRhoGEF2 encodes a member of the Dbl family of oncogenes and controls cell shape changes during gastrulation in Drosophila Genes & Dev., January 15, 1998; 12(2): 274 - 284. [Abstract] [Full Text]
|
|
|
|
|
|
C. R. Davis, T. J. Richman, S. B. Deliduka, J. O. Blaisdell, C. C. Collins, and D. I. Johnson Analysis of the Mechanisms of Action of the Saccharomyces cerevisiae Dominant Lethal cdc42G12V and Dominant Negative cdc42D118A Mutations J. Biol. Chem., January 9, 1998; 273(2): 849 - 858. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Li, B. Zhang, and Y. Zheng Structural Determinants Required for the Interaction between Rho GTPase and the GTPase-activating Domain of p190 J. Biol. Chem., December 26, 1997; 272(52): 32830 - 32835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-C. Chen, Y.-J. Kim, and C. S.M. Chan The Cdc42 GTPase-associated proteins Gic1 and Gic2 are required for polarized cell growth in Saccharomyces cerevisiae Genes & Dev., November 15, 1997; 11(22): 2958 - 2971. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Yang and R. A. Cerione Cloning and Characterization of a Novel Cdc42-associated Tyrosine Kinase, ACK-2, from Bovine Brain J. Biol. Chem., October 3, 1997; 272(40): 24819 - 24824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Manning, R. Padmanabha, and M. Snyder The Rho-GEF Rom2p Localizes to Sites of Polarized Cell Growth and Participates in Cytoskeletal Functions in Saccharomyces cerevisiae Mol. Biol. Cell, October 1, 1997; 8(10): 1829 - 1844. [Abstract] [Full Text]
|
|
|
|
|
|
L. Van Aelst and C. D'Souza-Schorey Rho GTPases and signaling networks Genes & Dev., September 15, 1997; 11(18): 2295 - 2322. [Full Text] [PDF]
|
|
|
|
|
|
K. Wassmann and G. Ammerer Overexpression of the G1-cyclin Gene CLN2 Represses the Mating Pathway in Saccharomyces cerevisiae at the Level of the MEKK Ste11 J. Biol. Chem., May 16, 1997; 272(20): 13180 - 13188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-O. Park, E. Bi, J. R. Pringle, and I. Herskowitz Two active states of the Ras-related Bud1/Rsr1 protein bind to different effectors to determine yeast cell polarity PNAS, April 29, 1997; 94(9): 4463 - 4468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Michiels, J. C. Stam, P. L. Hordijk, R. A. v. d. Kammen, L. R.-V. Stalle, C. A. Feltkamp, and J. G. Collard Regulated Membrane Localization of Tiam1, Mediated by the NH2-terminal Pleckstrin Homology Domain, Is Required for Rac-dependent Membrane Ruffling and C-Jun NH2-terminal Kinase Activation J. Cell Biol., April 21, 1997; 137(2): 387 - 398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Li and Y. Zheng Residues of the Rho Family GTPases Rho and Cdc42 That Specify Sensitivity to Dbl-like Guanine Nucleotide Exchange Factors J. Biol. Chem., February 21, 1997; 272(8): 4671 - 4679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zheng, D. J. Fischer, M. F. Santos, G. Tigyi, N. G. Pasteris, J. L. Gorski, and Y. Xu The Faciogenital Dysplasia Gene Product FGD1 Functions as a Cdc42Hs-specific Guanine-Nucleotide Exchange Factor J. Biol. Chem., December 27, 1996; 271(52): 33169 - 33172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Glaven, I. P. Whitehead, T. Nomanbhoy, R. Kay, and R. A. Cerione Lfc and Lsc Oncoproteins Represent Two New Guanine Nucleotide Exchange Factors for the Rho GTP-binding Protein J. Biol. Chem., November 1, 1996; 271(44): 27374 - 27381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Sohrmann, C Fankhauser, C Brodbeck, and V Simanis The dmf1/mid1 gene is essential for correct positioning of the division septum in fission yeast. Genes & Dev., November 1, 1996; 10(21): 2707 - 2719. [Abstract] [PDF]
|
|
|
|
|
|
J. W. Erickson, C.-j. Zhang, R. A. Kahn, T. Evans, and R. A. Cerione Mammalian Cdc42 Is a Brefeldin A-sensitive Component of the Golgi Apparatus J. Biol. Chem., October 25, 1996; 271(43): 26850 - 26854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zheng, J. A. Glaven, W. J. Wu, and R. A. Cerione Phosphatidylinositol 4,5-bisphosphate Provides an Alternative to Guanine Nucleotide Exchange Factors by Stimulating the Dissociation of GDP from Cdc42Hs J. Biol. Chem., September 27, 1996; 271(39): 23815 - 23819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zheng, D. Zangrilli, R. A. Cerione, and A. Eva The Pleckstrin Homology Domain Mediates Transformation by Oncogenic Dbl through Specific Intracellular Targeting J. Biol. Chem., August 9, 1996; 271(32): 19017 - 19020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. P. Whitehead, R. Khosravi-Far, H. Kirk, G. Trigo-Gonzalez, C. J. Der, and R. Kay Expression Cloning of lsc, a Novel Oncogene with Structural Similarities to the Dbl Family of Guanine Nucleotide Exchange Factors J. Biol. Chem., August 2, 1996; 271(31): 18643 - 18650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N Bonnefoy-Berard, A Munshi, I Yron, S Wu, T. Collins, M De |
