| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 269, Issue 4, 2377-2379, Jan, 1994
AP Kloek, J Yang, FS Mathews, C Frieden and DE Goldberg
The parasitic nematode Ascaris suum has a gene encoding a two-domain
hemoglobin with remarkable oxygen avidity. The strong interaction with
oxygen is a consequence of a particularly slow oxygen off-rate. The single
polypeptide chain consists of two domains, each of which can be expressed
separately in Escherichia coli as a globin-like protein exhibiting oxygen
binding characteristics comparable with the native molecule. Site-directed
mutagenesis was performed on the gene segment encoding domain one. The E7
position, involved in forming a hydrogen bond with the liganded oxygen in
vertebrate globins, is a glutamine in both Ascaris domains. Conversion of
this residue to leucine or alanine produced a hemoglobin variant with an
oxygen off-rate 5- or 60-fold faster than that of unaltered domain one.
Replacement of the tyrosine B10 with either phenylalanine or leucine (as
found in vertebrate globins) yielded hemoglobin mutants with oxygen
off-rates 280- or 570- fold faster, approaching rates found with vertebrate
myoglobins. The data suggest that the distal glutamine hydrogen bonds with
the liganded oxygen and that the tyrosine B10 hydroxyl contributes an
additional hydrogen bond that appears substantially responsible for the
extreme oxygen avidity of Ascaris hemoglobin.
The tyrosine B10 hydroxyl is crucial for oxygen avidity of Ascaris hemoglobin
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. K. Das, S. Dewilde, J. M. Friedman, L. Moens, and D. L. Rousseau Multiple Active Site Conformers in the Carbon Monoxide Complexes of Trematode Hemoglobins J. Biol. Chem., April 28, 2006; 281(17): 11471 - 11479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Miranda, D. H. Maillett, J. Soman, and J. S. Olson Thermoglobin, Oxygen-avid Hemoglobin in a Bacterial Hyperthermophile J. Biol. Chem., November 4, 2005; 280(44): 36754 - 36761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pesce, M. Nardini, P. Ascenzi, E. Geuens, S. Dewilde, L. Moens, M. Bolognesi, A. F. Riggs, A. Hale, P. Deng, et al. Thr-E11 Regulates O2 Affinity in Cerebratulus lacteus Mini-hemoglobin J. Biol. Chem., August 6, 2004; 279(32): 33662 - 33672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. K. Das, U. Samuni, Y. Lin, D. E. Goldberg, D. L. Rousseau, and J. M. Friedman Distal Heme Pocket Conformers of Carbonmonoxy Derivatives of Ascaris Hemoglobin: EVIDENCE OF CONFORMATIONAL TRAPPING IN POROUS SOL-GEL MATRICES J. Biol. Chem., March 12, 2004; 279(11): 10433 - 10441. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Weber and S. N. Vinogradov Nonvertebrate Hemoglobins: Functions and Molecular Adaptations Physiol Rev, April 1, 2001; 81(2): 569 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Gardner, L. A. Martin, P. R. Gardner, Y. Dou, and J. S. Olson Steady-state and Transient Kinetics of Escherichia coli Nitric-oxide Dioxygenase (Flavohemoglobin). THE B10 TYROSINE HYDROXYL IS ESSENTIAL FOR DIOXYGEN BINDING AND CATALYSIS J. Biol. Chem., April 21, 2000; 275(17): 12581 - 12589. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Nguyen, Z. Xia, F. Cutruzzola, C. T. Allocatelli, M. Brunori, and G. N. La Mar Solution 1H NMR Study of the Influence of Distal Hydrogen Bonding and N Terminus Acetylation on the Active Site Electronic and Molecular Structure of Aplysia limacina Cyanomet Myoglobin J. Biol. Chem., January 14, 2000; 275(2): 742 - 751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Xia, W. Zhang, B. D. Nguyen, G. N. L. Mar, A. P. Kloek, and D. E. Goldberg 1H NMR Investigation of the Distal Hydrogen Bonding Network and Ligand Tilt in the Cyanomet Complex of Oxygen-avid Ascaris suum Hemoglobin J. Biol. Chem., November 5, 1999; 274(45): 31819 - 31826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. I. Alayash, B. A. B. Ryan, R. F. Eich, J. S. Olson, and R. E. Cashon Reactions of Sperm Whale Myoglobin with Hydrogen Peroxide. EFFECTS OF DISTAL POCKET MUTATIONS ON THE FORMATION AND STABILITY OF THE FERRYL INTERMEDIATE J. Biol. Chem., January 22, 1999; 274(4): 2029 - 2037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tamburrini, M. Romano, V. Carratore, A. Kunzmann, M. Coletta, and G. di Prisco The Hemoglobins of the Antarctic Fishes Artedidraco orianae and Pogonophryne scotti. AMINO ACID SEQUENCE, LACK OF COOPERATIVITY, AND LIGAND BINDING PROPERTIES J. Biol. Chem., December 4, 1998; 273(49): 32452 - 32459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Minning and D. E. Goldberg Determinants of Ascaris Hemoglobin Octamer Formation J. Biol. Chem., December 4, 1998; 273(49): 32644 - 32649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. G. Duff, J. B. Wittenberg, and R. D. Hill Expression, Purification, and Properties of Recombinant Barley (Hordeum sp.) Hemoglobin. OPTICAL SPECTRA AND REACTIONS WITH GASEOUS LIGANDS J. Biol. Chem., July 4, 1997; 272(27): 16746 - 16752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zhang, K. A. Rashid, M. Haque, A. H. Siddiqi, S. N. Vinogradov, L. Moens, and G. N.L. Mar Solution of 1H NMR Structure of the Heme Cavity in the Oxygen-avid Myoglobin from the Trematode Paramphistomum epiclitum J. Biol. Chem., January 31, 1997; 272(5): 3000 - 3006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Huang, J. Huang, A. P. Kloek, D. E. Goldberg, and J. M. Friedman Hydrogen Bonding of Tyrosine B10 to Heme-bound Oxygen in Ascaris Hemoglobin J. Biol. Chem., January 12, 1996; 271(2): 958 - 962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Minning, A. P. Kloek, J. Yang, F. S. Mathews, and D. E. Goldberg Subunit Interactions in Ascaris Hemoglobin Octamer Formation J. Biol. Chem., September 22, 1995; 270(38): 22248 - 22253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mukai, C. E. Mills, R. K. Poole, and S.-R. Yeh Flavohemoglobin, a Globin with a Peroxidase-like Catalytic Site J. Biol. Chem., March 2, 2001; 276(10): 7272 - 7277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Draghi, A. E. Miele, C. Travaglini-Allocatelli, B. Vallone, M. Brunori, Q. H. Gibson, and J. S. Olson Controlling Ligand Binding in Myoglobin by Mutagenesis J. Biol. Chem., February 22, 2002; 277(9): 7509 - 7519. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
