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J. Biol. Chem., Vol. 269, Issue 4, 2440-2446, 01, 1994
JR Gum Jr, JW Hicks, NW Toribara, B Siddiki and YS Kim
Secretory mucins consist of a protein backbone that is catenated by
disulfide bonds, heavily O-glycosylated, and packaged into storage granules
prior to release from cells. In this paper, we identify and sequence cDNAs
that encode the amino terminus of the MUC2 protein, a major form of mucin
found in human intestines and airways. The protein sequence was found to
contain a repetitive element of approximately 350 amino acids with
considerable sequence similarity to the D domains of prepro-von Willebrand
factor. A total of four of these D domains were found to be present in the
MUC2 protein, three in the amino-terminal region, and one in the
carboxyl-terminal region. Prepro-von Willebrand factor contains four D
domains itself, and the overall positioning of the D domains in the two
proteins is similar. Prepro-von Willebrand factor contains a 741 residue
pro-protein that has been implicated in both the disulfide-linked
oligomerization of von Willebrand factor and its packaging into secretory
vacuoles. A similar region is present in the MUC2 amino terminus sequence,
suggesting that the mechanisms involved in the polymerization and packaging
of MUC2 may parallel those already described for von Willebrand factor.
Molecular cloning of human intestinal mucin (MUC2) cDNA. Identification of the amino terminus and overall sequence similarity to prepro-von Willebrand factor
Gastrointestinal Research Laboratory (151M2), Department of Veterans Affairs Medical Center, San Francisco, California.
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|
|
|
|
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|
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|
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|
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|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
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|
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|
|
|
|
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|
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|
|
|
|
 |
|
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|
|
|
|
 |
|
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|
|
|
|
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|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
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|
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|
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|
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J. S. Koo, J.-H. Yoon, T. Gray, D. Norford, A. M. Jetten, and P. Nettesheim Restoration of the Mucous Phenotype by Retinoic Acid in Retinoid-Deficient Human Bronchial Cell Cultures: Changes in Mucin Gene Expression Am. J. Respir. Cell Mol. Biol., January 1, 1999; 20(1): 43 - 52. [Abstract] [Full Text]
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B. J.-W. Van Klinken, A. W. C. Einerhand, L. A. Duits, M. K. Makkink, K. M. A. J. Tytgat, I. B. Renes, M. Verburg, H. A. Buller, and J. Dekker Gastrointestinal expression and partial cDNA cloning of murine Muc2 Am J Physiol Gastrointest Liver Physiol, January 1, 1999; 276(1): G115 - G124. [Abstract] [Full Text] [PDF]
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 I. K. Gipson, S. Spurr-Michaud, R. Moccia, Q. Zhan, N. Toribara, S. B. Ho, A. R. Gargiulo, and J. A. Hill III MUC4 and MUC5B Transcripts Are the Prevalent Mucin Messenger Ribonucleic Acids of the Human Endocervix Biol Reprod, January 1, 1999; 60(1): 58 - 64. [Abstract] [Full Text]
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N. Asker, M. A. B. Axelsson, S.-O. Olofsson, and G. C. Hansson Dimerization of the Human MUC2 Mucin in the Endoplasmic Reticulum Is Followed by a N-Glycosylation-dependent Transfer of the Mono- and Dimers to the Golgi Apparatus J. Biol. Chem., July 24, 1998; 273(30): 18857 - 18863. [Abstract] [Full Text] [PDF]
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J. Perez-Vilar, A. E. Eckhardt, A. DeLuca, and R. L. Hill Porcine Submaxillary Mucin Forms Disulfide-linked Multimers through Its Amino-terminal D-domains J. Biol. Chem., June 5, 1998; 273(23): 14442 - 14449. [Abstract] [Full Text] [PDF]
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J.-D. Li, W. Feng, M. Gallup, J.-H. Kim, J. Gum, Y. Kim, and C. Basbaum Activation of NF-kappa B via a Src-dependent Ras-MAPK-pp90rsk pathway is required for Pseudomonas aeruginosa-induced mucin overproduction in epithelial cells PNAS, May 12, 1998; 95(10): 5718 - 5723. [Abstract] [Full Text] [PDF]
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D. Li, M. Gallup, N. Fan, D. E. Szymkowski, and C. B. Basbaum Cloning of the Amino-terminal and |
