| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 269, Issue 40, 24534-24537, 10, 1994
N Calakos and RH Scheller
The synaptic vesicle membrane protein VAMP (vesicle-associated membrane
protein or synaptobrevin) has been implicated in synaptic vesicle docking
and fusion. Synaptophsin (p38), also a synaptic vesicle membrane protein,
has four transmembrane domains and may function as a gap junction-like pore
or channel. Here we report evidence for a direct interaction between VAMP
and synaptophysin using chemical cross-linking followed by the
identification of immunoreactive protein complexes. A prominent complex of
56 kDa was found to consist of VAMP and synaptophysin. Furthermore, we
demonstrate that this VAMP-synaptophysin complex is enriched in the
synaptic vesicle fraction of rat brain, is independent of detergent
solubilization, and is present in PC12 cells subjected to in vivo
cross-linking.
Vesicle-associated membrane protein and synaptophysin are associated on the synaptic vesicle
Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University Medical Center, California 94305.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. J. Siddiqui, O. Vites, A. Stein, R. Heintzmann, R. Jahn, and D. Fasshauer Determinants of Synaptobrevin Regulation in Membranes Mol. Biol. Cell, June 1, 2007; 18(6): 2037 - 2046. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. P. Jensen, A. G. Filoteo, T. Knopfel, and R. M. Empson Presynaptic plasma membrane Ca2+ ATPase isoform 2a regulates excitatory synaptic transmission in rat hippocampal CA3 J. Physiol., February 15, 2007; 579(1): 85 - 99. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Abraham, H. Hutter, M. T. Palfreyman, G. Spatkowski, R. M. Weimer, R. Windoffer, E. M. Jorgensen, and R. E. Leube Synaptic tetraspan vesicle membrane proteins are conserved but not needed for synaptogenesis and neuronal function in Caenorhabditis elegans PNAS, May 23, 2006; 103(21): 8227 - 8232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ungermann and D. Langosch Functions of SNAREs in intracellular membrane fusion and lipid bilayer mixing J. Cell Sci., September 1, 2005; 118(17): 3819 - 3828. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gurkan, H. Lapp, C. Alory, A. I. Su, J. B. Hogenesch, and W. E. Balch Large-Scale Profiling of Rab GTPase Trafficking Networks: The Membrome Mol. Biol. Cell, August 1, 2005; 16(8): 3847 - 3864. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Bonanomi, M. Pennuto, M. Rigoni, O. Rossetto, C. Montecucco, and F. Valtorta Taipoxin Induces Synaptic Vesicle Exocytosis and Disrupts the Interaction of Synaptophysin I with VAMP2 Mol. Pharmacol., June 1, 2005; 67(6): 1901 - 1908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, W. G. Brake, R. D. Romeo, J. C. Dunlop, M. Gordon, R. Buzescu, A. M. Magarinos, P. B. Allen, P. Greengard, V. Luine, et al. Estrogen alters hippocampal dendritic spine shape and enhances synaptic protein immunoreactivity and spatial memory in female mice PNAS, February 17, 2004; 101(7): 2185 - 2190. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. Rau, D. B. Dubal, M. Bottner, L. M. Gerhold, and P. M. Wise Estradiol Attenuates Programmed Cell Death after Stroke-Like Injury J. Neurosci., December 10, 2003; 23(36): 11420 - 11426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pennuto, D. Bonanomi, F. Benfenati, and F. Valtorta Synaptophysin I Controls the Targeting of VAMP2/Synaptobrevin II to Synaptic Vesicles Mol. Biol. Cell, December 1, 2003; 14(12): 4909 - 4919. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. W. Rau, D. B. Dubal, M. Bottner, and P. M. Wise Estradiol Differentially Regulates c-Fos after Focal Cerebral Ischemia J. Neurosci., November 19, 2003; 23(33): 10487 - 10494. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Gincel and V. Shoshan-Barmatz The Synaptic Vesicle Protein Synaptophysin: Purification and Characterization of Its Channel Activity Biophys. J., December 1, 2002; 83(6): 3223 - 3229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pennuto, D. Dunlap, A. Contestabile, F. Benfenati, and F. Valtorta Fluorescence Resonance Energy Transfer Detection of Synaptophysin I and Vesicle-associated Membrane Protein 2 Interactions during Exocytosis from Single Live Synapses Mol. Biol. Cell, August 1, 2002; 13(8): 2706 - 2717. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Daly and E. B. Ziff Ca2+-dependent Formation of a Dynamin-Synaptophysin Complex. POTENTIAL ROLE IN SYNAPTIC VESICLE ENDOCYTOSIS J. Biol. Chem., March 8, 2002; 277(11): 9010 - 9015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bacci, S. Coco, E. Pravettoni, U. Schenk, S. Armano, C. Frassoni, C. Verderio, P. De Camilli, and M. Matteoli Chronic Blockade of Glutamate Receptors Enhances Presynaptic Release and Downregulates the Interaction between Synaptophysin-Synaptobrevin-Vesicle-Associated Membrane Protein 2 J. Neurosci., September 1, 2001; 21(17): 6588 - 6596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhao and M. L. Nonet A Conserved Mechanism of Synaptogyrin Localization Mol. Biol. Cell, August 1, 2001; 12(8): 2275 - 2289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. H. Chamberlain, R. D. Burgoyne, and G. W. Gould SNARE proteins are highly enriched in lipid rafts in PC12 cells: Implications for the spatial control of exocytosis PNAS, April 25, 2001; (2001) 91502398. [Abstract] [Full Text]
|
|
|
|
 |
|
 W. G. Brake, S. E. Alves, J. C. Dunlop, S. J. Lee, K. Bulloch, P. B. Allen, P. Greengard, and B. S. McEwen Novel Target Sites for Estrogen Action in the Dorsal Hippocampus: An Examination of Synaptic Proteins Endocrinology, March 1, 2001; 142(3): 1284 - 1289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. A. Braun and D. V. Madison A Novel SNAP25-Caveolin Complex Correlates with the Onset of Persistent Synaptic Potentiation J. Neurosci., August 15, 2000; 20(16): 5997 - 6006. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Schiavo, M. Matteoli, and C. Montecucco Neurotoxins Affecting Neuroexocytosis Physiol Rev, April 1, 2000; 80(2): 717 - 766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Yang, M. Steegmaier, L. C. Gonzalez , Jr., and R. H. Scheller nSec1 Binds a Closed Conformation of Syntaxin1A J. Cell Biol., January 24, 2000; 148(2): 247 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Brooks, P. E. Scherer, K. Cleveland, J. L. Whittemore, H. F. Lodish, and B. Cheatham Pantophysin Is a Phosphoprotein Component of Adipocyte Transport Vesicles and Associates with GLUT4-containing Vesicles J. Biol. Chem., January 21, 2000; 275(3): 2029 - 2036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. D. Pozzo-Miller, W. Gottschalk, L. Zhang, K. McDermott, J. Du, R. Gopalakrishnan, C. Oho, Z.-H. Sheng, and B. Lu Impairments in High-Frequency Transmission, Synaptic Vesicle Docking, and Synaptic Protein Distribution in the Hippocampus of BDNF Knockout Mice J. Neurosci., June 15, 1999; 19(12): 4972 - 4983. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Lustgarten and J. E. Gerst Yeast VSM1 Encodes a v-SNARE Binding Protein That May Act as a Negative Regulator of Constitutive Exocytosis Mol. Cell. Biol., June 1, 1999; 19(6): 4480 - 4494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Steegmaier, J. Klumperman, D. L. Foletti, J.-S. Yoo, and R. H. Scheller Vesicle-associated Membrane Protein 4 is Implicated in Trans-Golgi Network Vesicle Trafficking Mol. Biol. Cell, June 1, 1999; 10(6): 1957 - 1972. [Abstract] [Full Text]
|
|
|
|
|
|
A. Becher, A. Drenckhahn, I. Pahner, M. Margittai, R. Jahn, and G. Ahnert-Hilger The Synaptophysin-Synaptobrevin Complex: a Hallmark of Synaptic Vesicle Maturation J. Neurosci., March 15, 1999; 19(6): 1922 - 1931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F Peter, S. Wong, V. Subramaniam, B. Tang, and W Hong Alpha-SNAP but not gamma-SNAP is required for ER-Golgi transport after vesicle budding and the Rab1-requiring step but before the EGTA-sensitive step J. Cell Sci., January 9, 1998; 111(17): 2625 - 2633. [Abstract] [PDF]
|
|
|
|
|
|
G. Thoidis, P. Chen, A. V. Pushkin, G. Vallega, S. E. Leeman, R. E. Fine, and K. V. Kandror Two distinct populations of synaptic-like vesicles from rat brain PNAS, January 6, 1998; 95(1): 183 - 188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. G. Annaert, B. Becker, U. Kistner, M. Reth, and R. Jahn Export of Cellubrevin from the Endoplasmic Reticulum Is Controlled by BAP31 J. Cell Biol., December 15, 1997; 139(6): 1397 - 1410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Hohne-Zell, A. Galler, W. Schepp, M. Gratzl, and C. Prinz Functional Importance of Synaptobrevin and SNAP-25 during Exocytosis of Histamine by Rat Gastric Enterochromaffin-Like Cells Endocrinology, December 1, 1997; 138(12): 5518 - 5526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Prekeris and D. M. Terrian Brain Myosin V Is a Synaptic Vesicle-associated Motor Protein: Evidence for a Ca2+-dependent Interaction with the Synaptobrevin-Synaptophysin Complex J. Cell Biol., June 30, 1997; 137(7): 1589 - 1601. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Gerst Conserved alpha -Helical Segments on Yeast Homologs of the Synaptobrevin/VAMP Family of v-SNAREs Mediate Exocytic Function J. Biol. Chem., June 27, 1997; 272(26): 16591 - 16598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Hao, N. Salem, X.-R. Peng, R. B. Kelly, and M. K. Bennett Effect of Mutations in Vesicle-Associated Membrane Protein (VAMP) on the Assembly of Multimeric Protein Complexes J. Neurosci., March 1, 1997; 17(5): 1596 - 1603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Igarashi, M. Tagaya, and Y. Komiya The Soluble N-Ethylmaleimide-Sensitive Factor Attached Protein Receptor Complex in Growth Cones: Molecular Aspects of the Axon Terminal Development J. Neurosci., February 15, 1997; 17(4): 1460 - 1470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Gutierrez, S. Viniegra, J. Rueda, A. V. Ferrer-Montiel, J. M. Canaves, and M. Montal A Peptide That Mimics the C-terminal Sequence of SNAP-25 Inhibits Secretory Vesicle Docking in Chromaffin Cells J. Biol. Chem., January 31, 1997; 272(5): 2634 - 2639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Schivell, R. H. Batchelor, and S. M. Bajjalieh Isoform-specific, Calcium-regulated Interaction of the Synaptic Vesicle Proteins SV2 and Synaptotagmin J. Biol. Chem., November 1, 1996; 271(44): 27770 - 27775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Williamson, J. L. Halpern, C. Montecucco, J. E. Brown, and E. A. Neale Clostridial Neurotoxins and Substrate Proteolysis in Intact Neurons J. Biol. Chem., March 29, 1996; 271(13): 7694 - 7699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Galli, P. S. McPherson, and P. De Camilli The V(0) Sector of the V-ATPase, Synaptobrevin, and Synaptophysin Are Associated on Synaptic Vesicles in a Triton X-100-resistant, Freeze-thawing Sensitive, Complex J. Biol. Chem., January 26, 1996; 271(4): 2193 - 2198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L Johannes, F Doussau, A Clabecq, J. Henry, F Darchen, and B Poulain Evidence for a functional link between Rab3 and the SNARE complex J. Cell Sci., January 12, 1996; 109(12): 2875 - 2884. [Abstract] [PDF]
|
|
|
|
|
|
F. Cornille, F. Deloye, M.-C. Fournié-Zaluski, B. P. Roques, and B. Poulain Inhibition of Neurotransmitter Release by Synthetic Proline-rich Peptides Shows That the N-terminal Domain of Vesicle-associated Membrane Protein/Synaptobrevin Is Critical for Neuro-exocytosis J. Biol. Chem., July 14, 1995; 270(28): 16826 - 16832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Bajjalieh and R. H. Scheller The Biochemistry of Neurotransmitter Secretion J. Biol. Chem., February 3, 1995; 270(5): 1971 - 1974. [Full Text] [PDF]
|
|
|
|
 |
|
 A.E. Ting and R.H. Scheller Insights into the Molecular Mechanisms of Synaptic Vesicle Docking and Fusion Cold Spring Harb Symp Quant Biol, January 1, 1995; 60(0): 361 - 369. [Abstract] [PDF]
|
|
|
|
|
|
Y. Kaneda, F. Grote, F. Bonzelius, C. Desnos, G. Herman, L. Clift-O'Grady, and R.B. Kelly Recycling of Synaptic Vesicle Membrane Components Cold Spring Harb Symp Quant Biol, January 1, 1995; 60(0): 379 - 387. [Abstract] [PDF]
|
|
|
|
|
|
Y. Takita, L. Engstrom, C. Ungermann, and K. W. Cunningham Inhibition of the Ca2+-ATPase Pmc1p by the v-SNARE Protein Nyv1p J. Biol. Chem., February 23, 2001; 276(9): 6200 - 6206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Tartaglia, J. Du, W. J. Tyler, E. Neale, L. Pozzo-Miller, and B. Lu Protein Synthesis-dependent and -independent Regulation of Hippocampal Synapses by Brain-derived Neurotrophic Factor J. Biol. Chem., September 28, 2001; 276(40): 37585 - 37593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Laage, J. Rohde, B. Brosig, and D. Langosch A Conserved Membrane-spanning Amino Acid Motif Drives Homomeric and Supports Heteromeric Assembly of Presynaptic SNARE Proteins J. Biol. Chem., June 2, 2000; 275(23): 17481 - 17487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. H. Chamberlain, R. D. Burgoyne, and G. W. Gould SNARE proteins are highly enriched in lipid rafts in PC12 cells: Implications for the spatial control of exocytosis PNAS, May 8, 2001; 98(10): 5619 - 5624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. El Far and H. Betz Synaptophysins: vesicular cation channels? J. Physiol., March 1, 2002; 539(2): 332 - 332. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
