| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 269, Issue 40, 24586-24595, Oct, 1994
TV Brennan, JW Anderson, Z Jia, EB Waygood and S Clarke
The non-enzymatic deamidation at residues Asn-12 and Asn-38 of Escherichia
coli phosphocarrier protein, HPr, and the repair of the resulting
L-isoaspartyl (or beta-aspartyl) derivatives, HPr-1 and HPr- 2, by
recombinant human S-adenosylmethionine-dependent L-isoaspartate-
(D-aspartate) O-methyltransferase (EC 2.1.1.77) were investigated. HPr is a
component of the bacterial phosphoenolpyruvate:sugar phosphotransferase
system that is involved in the concomitant translocation and
phosphorylation of many hexose sugars. The major products of the
deamidation reaction, L-isoaspartyl (or beta-aspartyl) residues at
positions 12 and 38, were found to be substrates for the L-
isoaspartate-(D-aspartate) O-methyltransferase, an enzyme active on a wide
variety of peptides and proteins containing these abnormal residues. This
enzyme has been shown to catalyze the first step in a process that can
convert L-isoaspartyl residues in peptides to normal L- aspartyl residues.
The affinity of a recombinant human methyltransferase for HPr-1, a form
deamidated at Asn-38, was relatively poor (Km = 3.6 mM), while a greater
affinity was found for HPr-2, a form deamidated at both Asn-12 and Asn-38
(Km = 197 microM). When HPr-2 was incubated with S-adenosylmethionine and
the methyltransferase, the bulk of the L-isoaspartyl residues at position
12 was converted to L-aspartyl residues. The major-by-product was the D-
isoaspartyl form. The conversion of L-isoaspartyl residues at position 38
to L-aspartyl residues was less complete, reflecting the lower affinity of
the methyltransferase for this site. The phosphohydrolysis activity of the
repaired form was found to be midway between the form containing only
L-aspartyl residues at positions 12 and 38 and the deamidated HPr-2 form.
Repair of spontaneously deamidated HPr phosphocarrier protein catalyzed by the L-isoaspartate-(D-aspartate) O-methyltransferase
Department of Chemistry and Biochemistry, University of California, Los Angeles 90024-1569.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  V. Vigneswara, J. D. Lowenson, C. D. Powell, M. Thakur, K. Bailey, S. Clarke, D. E. Ray, and W. G. Carter Proteomic Identification of Novel Substrates of a Protein Isoaspartyl Methyltransferase Repair Enzyme J. Biol. Chem., October 27, 2006; 281(43): 32619 - 32629. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-L. Yang, H. A. Doyle, R. J. Gee, J. D. Lowenson, S. Clarke, B. R. Lawson, D. W. Aswad, and M. J. Mamula Intracellular Protein Modification Associated with Altered T Cell Functions in Autoimmunity J. Immunol., October 1, 2006; 177(7): 4541 - 4549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Reissner, M. V. Paranandi, T. M. Luc, H. A. Doyle, M. J. Mamula, J. D. Lowenson, and D. W. Aswad Synapsin I Is a Major Endogenous Substrate for Protein L-Isoaspartyl Methyltransferase in Mammalian Brain J. Biol. Chem., March 31, 2006; 281(13): 8389 - 8398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Xu, M. P. Belcastro, S. T. Villa, R. D. Dinkins, S. G. Clarke, and A. B. Downie A Second Protein L-Isoaspartyl Methyltransferase Gene in Arabidopsis Produces Two Transcripts Whose Products Are Sequestered in the Nucleus Plant Physiology, September 1, 2004; 136(1): 2652 - 2664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kindrachuk, J. Parent, G. F. Davies, M. Dinsmore, S. Attah-Poku, and S. Napper Overexpression of L-Isoaspartate O-Methyltransferase in Escherichia coli Increases Heat Shock Survival by a Mechanism Independent of Methyltransferase Activity J. Biol. Chem., December 19, 2003; 278(51): 50880 - 50886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Athmer, J. Kindrachuk, F. Georges, and S. Napper The Influence of Protein Structure on the Products Emerging from Succinimide Hydrolysis J. Biol. Chem., August 16, 2002; 277(34): 30502 - 30507. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. E. Robinson and A. B. Robinson Deamidation of human proteins PNAS, October 12, 2001; (2001) 221463198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. E. Robinson and A. B. Robinson Prediction of protein deamidation rates from primary and three-dimensional structure PNAS, April 10, 2001; 98(8): 4367 - 4372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Thapar, A.-K. Kim, and S. Clarke Distinct Patterns of Expression But Similar Biochemical Properties of Protein L-Isoaspartyl Methyltransferase in Higher Plants Plant Physiology, February 1, 2001; 125(2): 1023 - 1035. [Abstract] [Full Text]
|
|
|
|
|
|
M. J. Mamula, R. J. Gee, J. I. Elliott, A. Sette, S. Southwood, P.-J. Jones, and P. R. Blier Isoaspartyl Post-translational Modification Triggers Autoimmune Responses to Self-proteins J. Biol. Chem., August 6, 1999; 274(32): 22321 - 22327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Napper, L. T. J. Delbaere, and E. B. Waygood The Aspartyl Replacement of the Active Site Histidine in Histidine-containing Protein, HPr, of the Escherichia coli Phosphoenolpyruvate:Sugar Phosphotransferase System Can Accept and Donate a Phosphoryl Group. SPONTANEOUS DEPHOSPHORYLATION OF ACYL-PHOSPHATE AUTOCATALYZES AN INTERNAL CYCLIZATION J. Biol. Chem., July 30, 1999; 274(31): 21776 - 21782. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kim, J. D. Lowenson, S. Clarke, and S. G. Young Phenotypic Analysis of Seizure-prone Mice Lacking L-Isoaspartate (D-Aspartate) O-Methyltransferase J. Biol. Chem., July 16, 1999; 274(29): 20671 - 20678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. David, J. Keener, and D. W. Aswad Isoaspartate in Ribosomal Protein S11 of Escherichia coli J. Bacteriol., May 1, 1999; 181(9): 2872 - 2877. [Abstract] [Full Text]
|
|
|
|
|
|
C. L. David, J. Orpiszewski, X.-C. Zhu, K. J. Reissner, and D. W. Aswad Isoaspartate in Chrondroitin Sulfate Proteoglycans of Mammalian Brain J. Biol. Chem., November 27, 1998; 273(48): 32063 - 32070. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Szymanska, J. D. Leszyk, and C. M. O'Connor Carboxyl Methylation of Deamidated Calmodulin Increases Its Stability in Xenopus Oocyte Cytoplasm. IMPLICATIONS FOR PROTEIN REPAIR J. Biol. Chem., October 23, 1998; 273(43): 28516 - 28523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. O'Connor and C. M. O'Connor Complex Interactions of the Protein L-Isoaspartyl Methyltransferase and Calmodulin Revealed with the Yeast Two-hybrid System J. Biol. Chem., May 22, 1998; 273(21): 12909 - 12913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kim, J. D. Lowenson, D. C. MacLaren, S. Clarke, and S. G. Young Deficiency of a protein-repair enzyme results in the accumulation of altered proteins, retardation of growth, and fatal seizures in mice PNAS, June 10, 1997; 94(12): 6132 - 6137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Orpiszewski and D. W. Aswad High Mass Methyl-accepting Protein (HMAP), a Highly Effective Endogenous Substrate for Protein -Isoaspartyl Methyltransferase in Mammalian Brain J. Biol. Chem., September 20, 1996; 271(38): 22965 - 22968. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Young, W. G. Carter, H. A. Doyle, M. J. Mamula, and D. W. Aswad Structural Integrity of Histone H2B in Vivo Requires the Activity of Protein L-Isoaspartate O-Methyltransferase, a Putative Protein Repair Enzyme J. Biol. Chem., September 28, 2001; 276(40): 37161 - 37165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Thapar, S. C. Griffith, T. O. Yeates, and S. Clarke Protein Repair Methyltransferase from the Hyperthermophilic Archaeon Pyrococcus furiosus. UNUSUAL METHYL-ACCEPTING AFFINITY FOR D-ASPARTYL AND N-SUCCINYL-CONTAINING PEPTIDES J. Biol. Chem., January 4, 2002; 277(2): 1058 - 1065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. E. Robinson and A. B. Robinson Deamidation of human proteins PNAS, October 23, 2001; 98(22): 12409 - 12413. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
