HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Masuoka, J. Articles by Saltman, P. Search for Related Content PubMed PubMed Citation Articles by Masuoka, J. Articles by Saltman, P. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 269, Issue 41, 25557-25561, Oct, 1994

Zinc(II) and copper(II) binding to serum albumin. A comparative study of dog, bovine, and human albumin

J Masuoka and P Saltman
Department of Biology, University of California at San Diego, La Jolla 92093-0322.

Metal binding strategies employing low molecular weight chelators and equilibrium dialysis were used to investigate several unresolved aspects of zinc and copper binding to serum albumin. Direct measurement of histidine binding to bovine serum albumin when the histidine is presented either as a metal-chelate or alone provides no evidence for an albumin-metal-histidine ternary complex. Using previously determined intrinsic constants for Zn(II) and Cu(II), we have measured zinc binding to bovine serum albumin in the presence of saturating amounts of copper. The results of these experiments unambiguously show that zinc and copper bind at separate noninteracting sites on this protein. The intrinsic constants for zinc and copper binding to dog serum albumin have been determined. Contrary to previous reports, we find that dog serum albumin has a specific high affinity site for copper, log10K 10.17 for Cu(II) compared to 6.85 for Zn(II) at the separate site.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. J. Rowe and D. J. Bobilya Albumin Facilitates Zinc Acquisition by Endothelial Cells Experimental Biology and Medicine, July 1, 2000; 224(3): 178 - 186. [Abstract] [Full Text]