HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Des Rosiers, C. Articles by Brunengraber, H. Search for Related Content PubMed PubMed Citation Articles by Des Rosiers, C. Articles by Brunengraber, H. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 269, Issue 44, 27179-27182, 11, 1994

Reversibility of the mitochondrial isocitrate dehydrogenase reaction in the perfused rat liver. Evidence from isotopomer analysis of citric acid cycle intermediates

C Des Rosiers, CA Fernandez, F David and H Brunengraber
Department of Nutrition, University of Montreal, Quebec, Canada.

The reversal of the mitochondrial isocitrate dehydrogenase reaction was investigated in rat livers perfused with [U-13C5]glutamate or [U- 13C5]glutamine. The mass isotopomer distribution of citric acid cycle intermediates extracted from the livers was determined by gas chromatography-mass spectrometry. Citrate was enriched in an isotopomer containing five 13C. The formation of this isotopomer can only be explained by the reversal of the isocitrate dehydrogenase reaction. Calculation of kinetic parameters from the mass isotopomer data reveals a rapid interconversion of isocitrate and alpha-ketoglutarate. This interconversion results in an isotopic exchange between carbon 6 of citrate and mitochondrial CO2 that can affect the calculation of citric acid cycle kinetic parameters. Thus, the reversal of the isocitrate dehydrogenase reaction should be included in isotope labeling models of the citric acid cycle.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. Yang, T. Kasumov, R. S. Kombu, S.-H. Zhu, A. V. Cendrowski, F. David, V. E. Anderson, J. K. Kelleher, and H. Brunengraber Metabolomic and Mass Isotopomer Analysis of Liver Gluconeogenesis and Citric Acid Cycle: II. HETEROGENEITY OF METABOLITE LABELING PATTERN J. Biol. Chem., August 8, 2008; 283(32): 21988 - 21996. [Abstract] [Full Text] [PDF]

				L. Zhou, M. E. Cabrera, H. Huang, C. L. Yuan, D. K. Monika, N. Sharma, F. Bian, and W. C. Stanley Parallel activation of mitochondrial oxidative metabolism with increased cardiac energy expenditure is not dependent on fatty acid oxidation in pigs J. Physiol., March 15, 2007; 579(3): 811 - 821. [Abstract] [Full Text] [PDF]

				J. Schwender, Y. Shachar-Hill, and J. B. Ohlrogge Mitochondrial Metabolism in Developing Embryos of Brassica napus J. Biol. Chem., November 10, 2006; 281(45): 34040 - 34047. [Abstract] [Full Text] [PDF]

				I. C. Okere, T. A. McElfresh, D. Z. Brunengraber, W. Martini, J. P. Sterk, H. Huang, M. P. Chandler, H. Brunengraber, and W. C. Stanley Differential effects of heptanoate and hexanoate on myocardial citric acid cycle intermediates following ischemia-reperfusion J Appl Physiol, January 1, 2006; 100(1): 76 - 82. [Abstract] [Full Text] [PDF]

				N. Sharma, I. C. Okere, D. Z. Brunengraber, T. A. McElfresh, K. L. King, J. P. Sterk, H. Huang, M. P. Chandler, and W. C. Stanley Regulation of pyruvate dehydrogenase activity and citric acid cycle intermediates during high cardiac power generation J. Physiol., January 15, 2005; 562(2): 593 - 603. [Abstract] [Full Text] [PDF]

				M. J. MacDonald, L. A. Fahien, L. J. Brown, N. M. Hasan, J. D. Buss, and M. A. Kendrick Perspective: emerging evidence for signaling roles of mitochondrial anaplerotic products in insulin secretion Am J Physiol Endocrinol Metab, January 1, 2005; 288(1): E1 - E15. [Abstract] [Full Text] [PDF]

				B. Comte, G. Vincent, B. Bouchard, M. Benderdour, and C. Des Rosiers Reverse flux through cardiac NADP+-isocitrate dehydrogenase under normoxia and ischemia Am J Physiol Heart Circ Physiol, October 1, 2002; 283(4): H1505 - H1514. [Abstract] [Full Text] [PDF]

				B. Comte, G. Vincent, B. Bouchard, and C. D. Rosiers Probing the Origin of Acetyl-CoA and Oxaloacetate Entering the Citric Acid Cycle from the 13C Labeling of Citrate Released by Perfused Rat Hearts J. Biol. Chem., October 17, 1997; 272(42): 26117 - 26124. [Abstract] [Full Text] [PDF]

				S. Petersen, A. A. de Graaf, L. Eggeling, M. Mollney, W. Wiechert, and H. Sahm In Vivo Quantification of Parallel and Bidirectional Fluxes in the Anaplerosis of Corynebacterium glutamicum J. Biol. Chem., November 10, 2000; 275(46): 35932 - 35941. [Abstract] [Full Text] [PDF]