| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 269, Issue 44, 27186-27192, 11, 1994
PA Staubs, DR Reichart, AR Saltiel, KL Milarski, H Maegawa, P Berhanu, JM Olefsky and BL Seely
The insulin receptor is known to interact with the SH2 domain proteins p85
(the regulatory subunit of phosphatidylinositol 3-kinase), Syp (a tyrosine
phosphatase), and GAP (GTPase-activating protein). In this study, we mapped
the insulin receptor binding sites for each of these proteins by examining
the ability of phosphopeptides, corresponding to insulin receptor
phosphorylation sites, and mutant insulin receptors to inhibit an insulin
receptor-SH2 domain interaction. Precipitation of partially purified
insulin receptors by glutathione S-transferase fusion proteins containing
the N-terminal SH2 domains of p85 and GAP and both SH2 domains of Syp was
demonstrated. The effect of the addition of each phosphopeptide on insulin
receptor precipitation was tested. pY1322, the C-terminal insulin receptor
peptide, inhibited insulin receptor precipitation by both p85- and Syp-GST.
The NPXY internalization domain peptide inhibited insulin receptor
precipitation by GAP-GST. These data were confirmed by mutant insulin
receptor experiments. The insulin receptor C-terminal mutants, delta CT and
Y/F2, were not precipitated by p85- or Syp-GST and the NPXY mutant insulin
receptors, delta Ex16 and HI delta NPEY, were not precipitated by GAP-GST.
Therefore, we conclude that p85 and Syp bind to the insulin receptor C
terminus at tyrosine 1322 and GAP binds to the insulin receptor NPXY domain
at tyrosine 960.
Localization of the insulin receptor binding sites for the SH2 domain proteins p85, Syp, and GAP
Department of Medicine, University of California, San Diego 92093-0673.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Imhof, A.-S. Wavreille, A. May, M. Zacharias, S. Tridandapani, and D. Pei Sequence Specificity of SHP-1 and SHP-2 Src Homology 2 Domains: CRITICAL ROLES OF RESIDUES BEYOND THE pY+3 POSITION J. Biol. Chem., July 21, 2006; 281(29): 20271 - 20282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Mussig, H. Staiger, H. Fiedler, K. Moeschel, A. Beck, M. Kellerer, and H.-U. Haring Shp2 Is Required for Protein Kinase C-dependent Phosphorylation of Serine 307 in Insulin Receptor Substrate-1 J. Biol. Chem., September 23, 2005; 280(38): 32693 - 32699. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. M. Borisov, N. I. Markevich, J. B. Hoek, and B. N. Kholodenko Signaling through Receptors and Scaffolds: Independent Interactions Reduce Combinatorial Complexity Biophys. J., August 1, 2005; 89(2): 951 - 966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Mitra, X. Zheng, and M. P. Czech RNAi-based Analysis of CAP, Cbl, and CrkII Function in the Regulation of GLUT4 by Insulin J. Biol. Chem., September 3, 2004; 279(36): 37431 - 37435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Asante-Appiah and B. P. Kennedy Protein tyrosine phosphatases: the quest for negative regulators of insulin action Am J Physiol Endocrinol Metab, April 1, 2003; 284(4): E663 - E670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Marandi, N. De Keyser, A. Saliez, A.-S. Maernoudt, E. M. Sokal, C. Stilmant, M. H. Rider, and J.-P. Buts Insulin signal transduction in rat small intestine: role of MAP kinases in expression of mucosal hydrolases Am J Physiol Gastrointest Liver Physiol, February 1, 2001; 280(2): G229 - G240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Drake, A. Balbis, J. Wu, J. J. M. Bergeron, and B. I. Posner Association of phosphatidylinositol 3-kinase with the insulin receptor: compartmentation in rat liver Am J Physiol Endocrinol Metab, August 1, 2000; 279(2): E266 - E274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Soni, M. Lakkis, M. N. Poy, M. A. Fernström, and S. M. Najjar The Differential Effects of pp120 (Ceacam 1) on the Mitogenic Action of Insulin and Insulin-Like Growth Factor 1 Are Regulated by the Nonconserved Tyrosine 1316 in the Insulin Receptor Mol. Cell. Biol., June 1, 2000; 20(11): 3896 - 3905. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Morrison, L. Waggoner, L. Whitworth-Langley, and B. J. Stith Nongenomic Action of Progesterone: Activation of XenopusOocyte Phospholipase C through a Plasma Membrane-Associated Tyrosine Kinase Endocrinology, June 1, 2000; 141(6): 2145 - 2152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Nakashima, P. M. Sharma, T. Imamura, R. Bookstein, and J. M. Olefsky The Tumor Suppressor PTEN Negatively Regulates Insulin Signaling in 3T3-L1 Adipocytes J. Biol. Chem., April 21, 2000; 275(17): 12889 - 12895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Rondinone, E. Carvalho, T. Rahn, V. C. Manganiello, E. Degerman, and U. P. Smith Phosphorylation of PDE3B by Phosphatidylinositol 3-Kinase Associated with the Insulin Receptor J. Biol. Chem., March 31, 2000; 275(14): 10093 - 10098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kasus-Jacobi, D. Perdereau, C. Auzan, E. Clauser, E. Van Obberghen, F. Mauvais-Jarvis, J. Girard, and A.-F. Burnol Identification of the Rat Adapter Grb14 as an Inhibitor of Insulin Actions J. Biol. Chem., October 2, 1998; 273(40): 26026 - 26035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Kayali, J. Eichhorn, T. Haruta, A. J. Morris, J. G. Nelson, P. Vollenweider, J. M. Olefsky, and N. J. G. Webster Association of the Insulin Receptor with Phospholipase C-gamma (PLCgamma ) in 3T3-L1 Adipocytes Suggests a Role for PLCgamma in Metabolic Signaling by Insulin J. Biol. Chem., May 29, 1998; 273(22): 13808 - 13818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Najjar, C. V. Choice, P. Soni, C. M. Whitman, and M. N. Poy Effect of pp120 on Receptor-mediated Insulin Endocytosis Is Regulated by the Juxtamembrane Domain of the Insulin Receptor J. Biol. Chem., May 22, 1998; 273(21): 12923 - 12928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. K. Sung, W. S. Choi, and V. Sanchez-Margalet Guanosine Triphosphatase-Activating Protein-Associated Protein, But Not src-Associated Protein p68 in Mitosis, Is a Part of Insulin Signaling Complexes Endocrinology, May 1, 1998; 139(5): 2392 - 2398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang and H. Riedel Insulin-like Growth Factor-I Receptor and Insulin Receptor Association with a Src Homology-2 Domain-containing Putative Adapter J. Biol. Chem., February 6, 1998; 273(6): 3136 - 3139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Q. Dong, S. Farris, J. Christal, and F. Liu Site-Directed Mutagenesis and Yeast Two-Hybrid Studies of the Insulin and Insulin-Like Growth Factor-1 Receptors: The Src Homology-2 Domain-Containing Protein hGrb10 Binds to the Autophosphorylated Tyrosine Residues in the Kinase Domain of the Insulin Receptor Mol. Endocrinol., November 1, 1997; 11(12): 1757 - 1765. [Abstract] [Full Text]
|
|
|
|
|
|
A. Morrione, B. Valentinis, M. Resnicoff, S.-q. Xu, and R. Baserga The Role of mGrb10alpha in Insulin-like Growth Factor I-mediated Growth J. Biol. Chem., October 17, 1997; 272(42): 26382 - 26387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Berhanu, C. Anderson, M. Hickman, and T. P. Ciaraldi Insulin Signal Transduction by a Mutant Human Insulin Receptor Lacking the NPEY Sequence. EVIDENCE FOR AN ALTERNATE MITOGENIC SIGNALING PATHWAY THAT IS INDEPENDENT OF Shc PHOSPHORYLATION J. Biol. Chem., September 5, 1997; 272(36): 22884 - 22890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kasus-Jacobi, D. Perdereau, S. Tartare-Deckert, E. Van Obberghen, J. Girard, and A.-F. Burnol Evidence for a Direct Interaction between Insulin Receptor Substrate-1 and Shc J. Biol. Chem., July 4, 1997; 272(27): 17166 - 17170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. W. Brownsey, A. N. Boone, and M. F. Allard Actions of insulin on the mammalian heart: metabolism, pathology and biochemical mechanisms Cardiovasc Res, April 1, 1997; 34(1): 3 - 24. [Full Text] [PDF]
|
|
|
|
|
|
J. D. Frantz, S. Giorgetti-Peraldi, E. A. Ottinger, and S. E. Shoelson Human GRB-IRbeta /GRB10. SPLICE VARIANTS OF AN INSULIN AND GROWTH FACTOR RECEPTOR-BINDING PROTEIN WITH PH AND SH2 DOMAINS J. Biol. Chem., January 31, 1997; 272(5): 2659 - 2667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. K. Kole, A. S. Liotta, S. Kole, J. Roth, C. Montrose-Rafizadeh, and M. Bernier A Synthetic Peptide Derived from a COOH-terminal Domain of the Insulin Receptor Specifically Enhances Insulin Receptor Signaling J. Biol. Chem., December 6, 1996; 271(49): 31619 - 31626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. A. Ricketts, D. W. Rose, S. Shoelson, and J. M. Olefsky Functional Roles of the Shc Phosphotyrosine Binding and Src Homology 2Domains in Insulin and Epidermal Growth Factor Signaling J. Biol. Chem., October 18, 1996; 271(42): 26165 - 26169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ugi, H. Maegawa, A. Kashiwagi, M. Adachi, J. M. Olefsky, and R. Kikkawa Expression of Dominant Negative Mutant SHPTP2 Attenuates Phosphatidylinositol 3`-Kinase Activity via Modulation of Phosphorylation of Insulin Receptor Substrate-1 J. Biol. Chem., May 24, 1996; 271(21): 12595 - 12602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hansen, U. Svensson, J. Zhu, L. Laviola, F. Giorgino, G. Wolf, R. J. Smith, and H. Riedel Interaction between the Grb10 SH2 Domain and the Insulin Receptor Carboxyl Terminus J. Biol. Chem., April 12, 1996; 271(15): 8882 - 8886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kharitonenkov, Jür. Schnekenburger, Z. Chen, P. Knyazev, S. Ali, E. Zwick, M. White, and A. Ullrich Adapter Function of Protein-tyrosine Phosphatase 1D in Insulin Receptor/Insulin Receptor Substrate-1 Interaction J. Biol. Chem., December 8, 1995; 270(49): 29189 - 29193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Stein-Gerlach, A. Kharitonenkov, W. Vogel, S. Ali, and A. Ullrich Protein-tyrosine Phosphatase 1D Modulates Its Own State of Tyrosine Phosphorylation J. Biol. Chem., October 20, 1995; 270(42): 24635 - 24637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kosaki, T. S. Pillay, L. Xu, and N. J. G. Webster The B Isoform of the Insulin Receptor Signals More Efficiently Than the A Isoform in HepG2 Cells J. Biol. Chem., September 1, 1995; 270(35): 20816 - 20823. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Seely, D. R. Reichart, P. A. Staubs, B. H. Jhun, D. Hsu, H. Maegawa, K. L. Milarski, A. R. Saltiel, and J. M. Olefsky Localization of the Insulin-like Growth Factor I Receptor Binding Sites for the SH2 Domain Proteins p85, Syp, and GTPase Activating Protein J. Biol. Chem., August 11, 1995; 270(32): 19151 - 19157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. S. Pillay, T. Sasaoka, and J. M. Olefsky Insulin Stimulates the Tyrosine Dephosphorylation of pp125 Focal Adhesion Kinase J. Biol. Chem., January 20, 1995; 270(3): 991 - 994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Till, A. J. Ablooglu, M. Frankel, S. M. Bishop, R. A. Kohanski, and S. R. Hubbard Crystallographic and Solution Studies of an Activation Loop Mutant of the Insulin Receptor Tyrosine Kinase. INSIGHTS INTO KINASE MECHANISM J. Biol. Chem., March 23, 2001; 276(13): 10049 - 10055. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
