HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sapru, M. K. Articles by Goldman, D. Search for Related Content PubMed PubMed Citation Articles by Sapru, M. K. Articles by Goldman, D. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 269, Issue 45, 27811-27814, 11, 1994

Protein-tyrosine phosphatases specifically regulate muscle adult-type nicotinic acetylcholine receptor gene expression

MK Sapru, G Zhou and D Goldman
Mental Health Research Institute, University of Michigan, Ann Arbor 48109.

Innervation of skeletal muscles results in expression of adult-type nicotinic acetylcholine receptors (alpha 2 beta epsilon delta) beneath the neuromuscular junction. This local expression is largely a result of selective induction of adult-type nicotinic acetylcholine receptor (nAChR) genes in endplate-associated myonuclei. The molecular mechanism by which the nerve induces gene expression in these nuclei is not known. We have shown previously that ionophore-induced calcium influx across the plasma membrane preferentially decreases expression from the adult-type specific nAChR epsilon-subunit gene (Walke, W., Staple, J., Adams, L., Gnegy, M., Chahine, K., and Goldman, D. (1994) J. Biol. Chem. 269, 19447-19456). Here we provide evidence that the genes encoding adult-type nAChRs are specifically regulated by protein- tyrosine phosphatase activity. Orthovanadate, a specific protein- tyrosine phosphatase inhibitor, caused increased expression of the epsilon-subunit gene in rat primary myotubes and was able to completely block the suppressive effects of increased calcium influx on epsilon- subunit RNA expression. Overexpression of protein-tyrosine phosphatases selectively decreased expression from the adult-type nAChR genes with no effect on the embryonic-type specific gamma-subunit gene. These results demonstrate that protein-tyrosine phosphatases regulate mammalian adult-type nAChR gene expression and suggest a mechanism by which muscle innervation selectively regulates gene expression in endplate-associated myonuclei.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. Wang, J. T. Hackett, M. E. Cox, M. van Hoek, J. M. Lindstrom, and S. J. Parsons Regulation of the Neuronal Nicotinic Acetylcholine Receptor by Src Family Tyrosine Kinases J. Biol. Chem., March 5, 2004; 279(10): 8779 - 8786. [Abstract] [Full Text] [PDF]

				M. Tanowitz, J. Si, D.-H. Yu, G.-S. Feng, and L. Mei Regulation of Neuregulin-Mediated Acetylcholine Receptor Synthesis by Protein Tyrosine Phosphatase SHP2 J. Neurosci., November 1, 1999; 19(21): 9426 - 9435. [Abstract] [Full Text] [PDF]

				M. K. Sapru, S. K. Florance, C. Kirk, and D. Goldman Identification of a neuregulin and protein-tyrosine phosphatase response element in the nicotinic acetylcholine receptor varepsilon  subunit gene: Regulatory role of an Ets transcription factor PNAS, February 3, 1998; 95(3): 1289 - 1294. [Abstract] [Full Text] [PDF]