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J. Biol. Chem., Vol. 269, Issue 51, 32194-32200, Dec, 1994
M Desjardins, JE Celis, G van Meer, H Dieplinger, A Jahraus, G Griffiths and LA Huber
The transformation of newly formed phagosomes into mature phagolysosomes is
a process that involves a complex series of interactions between phagosomes
and other vacuolar organelles. The machinery required by phagosomes to
mediate these interactions is poorly understood. In this study, we allowed
human and various rodent cells to take up latex beads whose density
facilitates a simple purification of phagosomes using discontinuous sucrose
gradients. With this system, we initiated a systematic study of phagosome
proteins using two-dimensional gel electrophoresis and the currently
available two-dimensional gel protein data bases. By this approach, we were
able to recognize a group of polypeptides associated with mouse J774
phagosomes-phagolysosomes including annexin II, annexin VI, the beta-1 and
beta-2 subunits of trimeric G proteins, and a group of actin- binding
proteins. While the amount of annexin II associated to phagosomes was
similar at all times of latex internalization, the levels of annexin VI
were higher on late phagosomes. Phospholipid analysis of J774 phagosomes
isolated at early and late time points during phagolysosome formation also
revealed significant differences in their lipid composition. In the human
phagosomes, we resolved over 200 polypeptides on the two-dimensional gels.
These included the proteins described in the mouse, as well as 32
polypeptides that were found to be highly enriched in phagosomes, 15 of
which are not present in the current data bases. The results demonstrate
that the use of latex bead phagosomes is a powerful system to identify key
molecules involved in phagolysosome biogenesis.
Molecular characterization of phagosomes
European Molecular Biology Laboratory, Heidelberg, Germany.
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|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
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|
|
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 M. Desjardins and A. Descoteaux Inhibition of Phagolysosomal Biogenesis by the Leishmania Lipophosphoglycan J. Exp. Med., June 16, 1997; 185(12): 2061 - 2068. [Abstract] [Full Text] [PDF]
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M Desjardins, N. Nzala, R Corsini, and C Rondeau Maturation of phagosomes is accompanied by changes in their fusion properties and size-selective acquisition of solute materials from endosomes J. Cell Sci., January 9, 1997; 110(18): 2303 - 2314. [Abstract] [PDF]
|
|
|
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|
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M Diakonova, V Gerke, J Ernst, J. Liautard, G van der Vusse, and G Griffiths Localization of five annexins in J774 macrophages and on isolated phagosomes J. Cell Sci., January 5, 1997; 110(10): 1199 - 1213. [Abstract] [PDF]
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