JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Reed, V. S.
Right arrow Articles by Yang, D. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reed, V. S.
Right arrow Articles by Yang, D. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J. Biol. Chem., Vol. 269, Issue 52, 32932-32936, Dec, 1994

Mechanisms of the transfer of aminoacyl-tRNA from aminoacyl-tRNA synthetase to the elongation factor 1 alpha

VS Reed, ME Wastney and DC Yang
Department of Chemistry, Georgetown University, Washington, D.C. 20057.

Aspartylation of mammalian tRNAAsp by bacteria-expressed human aspartyl- tRNA synthetase (hDRS) was examined. The kinetics of the aspartylation of tRNA was consistent with the following reaction pathway, [formula: see text] where E, represents aspartyl-tRNA synthetase. A set of rate constants was obtained which fit single turnover time courses at varying concentrations of the enzyme, tRNA, and AMP using the SAAM program. The dissociation of Asp-tRNA (k3) was found to be rate limiting. The elongation factor 1 alpha (EF1 alpha) and GTP stimulated the hDRS aspartylation. The stimulation depended on the presence of both EF1 alpha and GTP. Kinetic analysis indicated that EF1 alpha formed a complex with the hDRS-Asp-tRNA complex and stimulated the dissociation of Asp-tRNA. In the presence of 0.5 M NH4Cl, which enhances the binding of Asp-tRNA by EF1 alpha, hDRS-bound Asp-tRNA can be transferred directly to EF1 alpha. The implications of these results on the function of the multi-tRNA synthetase complex will be discussed.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
S. B. Stephens and C. V. Nicchitta
Divergent Regulation of Protein Synthesis in the Cytosol and Endoplasmic Reticulum Compartments of Mammalian Cells
Mol. Biol. Cell, February 1, 2008; 19(2): 623 - 632.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. D. Hausmann, M. Praetorius-Ibba, and M. Ibba
An aminoacyl-tRNA synthetase:elongation factor complex for substrate channeling in archaeal translation
Nucleic Acids Res., September 25, 2007; 35(18): 6094 - 6102.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
E. C. Chan, S. Fraser, S. Yin, G. Yeo, K. Kwek, R. J. Fairclough, and R. Smith
Human Myometrial Genes Are Differentially Expressed in Labor: A Suppression Subtractive Hybridization Study
J. Clin. Endocrinol. Metab., June 1, 2002; 87(6): 2435 - 2441.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. E. Kim, K.-H. Kim, S. W. Lee, W. Seol, K. Shiba, and S. Kim
An elongation factor-associating domain is inserted into human cysteinyl-tRNA synthetase by alternative splicing
Nucleic Acids Res., August 1, 2000; 28(15): 2866 - 2872.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
H. Grosshans, E. Hurt, and G. Simos
An aminoacylation-dependent nuclear tRNA export pathway in yeast
Genes & Dev., April 1, 2000; 14(7): 830 - 840.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
S. G. Park, K. H. Jung, J. S. Lee, Y. J. Jo, H. Motegi, S. Kim, and K. Shiba
Precursor of Pro-apoptotic Cytokine Modulates Aminoacylation Activity of tRNA Synthetase
J. Biol. Chem., June 11, 1999; 274(24): 16673 - 16676.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. B. Rho, M. J. Kim, J. S. Lee, W. Seol, H. Motegi, S. Kim, and K. Shiba
Genetic dissection of protein-protein interactions in multi-tRNA synthetase complex
PNAS, April 13, 1999; 96(8): 4488 - 4493.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. S. Negrutskii, V. F. Shalak, P. Kerjan, A. V. El'skaya, and M. Mirande
Functional Interaction of Mammalian Valyl-tRNA Synthetase with Elongation Factor EF-1alpha in the Complex with EF-1H
J. Biol. Chem., February 19, 1999; 274(8): 4545 - 4550.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. B. Rho, J. S. Lee, E.-J. Jeong, K.-S. Kim, Y. G. Kim, and S. Kim
A Multifunctional Repeated Motif Is Present in Human Bifunctional tRNA Synthetase
J. Biol. Chem., May 1, 1998; 273(18): 11267 - 11273.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Stapulionis, S. Kolli, and M. P. Deutscher
Efficient Mammalian Protein Synthesis Requires an Intact F-Actin System
J. Biol. Chem., October 3, 1997; 272(40): 24980 - 24986.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Hammamieh and D. C. H. Yang
Magnesium Ion-mediated Binding to tRNA by an Amino-terminal Peptide of a Class II tRNA Synthetase
J. Biol. Chem., January 5, 2001; 276(1): 428 - 433.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1994 by the American Society for Biochemistry and Molecular Biology.