| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 269, Issue 6, 4409-4416, Feb, 1994
LH Davis and V Bennett
This study analyzed the complex interactions of intact spectrin with bovine
brain membranes by evaluating membrane associations of defined regions of
beta G spectrin, the subunit responsible for high affinity membrane
binding. Two regions of beta G spectrin were expressed in bacteria and
demonstrated to contain fully functional binding site(s) for a subset of
spectrin-binding sites in brain membranes depleted of peripheral proteins.
One region, located near the NH2 terminus, was comprised of 106-residue
repeats and required repeats 2-7 for full activity. The other binding
domain was located at the COOH terminus, which is the most variable between
beta G and beta R spectrins, is distinct from the 106-residue repeats, and
contains a pleckstrin homology domain. NH2-terminal beta spectrin
polypeptides interacted with a membrane site(s) that recognized both brain
and erythrocyte isoforms of spectrin, was inhibited by calcium/calmodulin,
and was not blocked by the COOH-terminal polypeptide. The COOH-terminal
region associated with a membrane site(s) that was specific for brain
spectrin, was not inhibited by calcium/calmodulin, and was not blocked by
the NH2-terminal polypeptide. These observations demonstrate membrane
association of spectrin with at least two independent sites, which differ
with regard to regulation by calcium/calmodulin and in selectivity for
spectrin isoforms.
Identification of two regions of beta G spectrin that bind to distinct sites in brain membranes
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Das, C. Base, D. Manna, W. Cho, and R. R. Dubreuil Unexpected Complexity in the Mechanisms That Target Assembly of the Spectrin Cytoskeleton J. Biol. Chem., May 2, 2008; 283(18): 12643 - 12653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Garbe, A. Das, R. R. Dubreuil, and G. J. Bashaw {beta}-Spectrin functions independently of Ankyrin to regulate the establishment and maintenance of axon connections in the Drosophila embryonic CNS Development, January 15, 2007; 134(2): 273 - 284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Das, C. Base, S. Dhulipala, and R. R. Dubreuil Spectrin functions upstream of ankyrin in a spectrin cytoskeleton assembly pathway J. Cell Biol., October 23, 2006; 175(2): 325 - 335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Williams, B. MacIver, E. A. Klipfell, and G. H. Thomas The C-terminal domain of Drosophila {beta}Heavy-spectrin exhibits autonomous membrane association and modulates membrane area J. Cell Sci., February 15, 2004; 117(5): 771 - 782. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. De Vos, J. Sable, K. E. Miller, and M. P. Sheetz Expression of Phosphatidylinositol (4,5) Bisphosphate-specific Pleckstrin Homology Domains Alters Direction But Not the Level of Axonal Transport of Mitochondria Mol. Biol. Cell, September 1, 2003; 14(9): 3636 - 3649. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E. Warner, C. DelloRusso, R. W. Crawford, I. N. Rybakova, J. R. Patel, J. M. Ervasti, and J. S. Chamberlain Expression of Dp260 in muscle tethers the actin cytoskeleton to the dystrophin-glycoprotein complex and partially prevents dystrophy Hum. Mol. Genet., May 1, 2002; 11(9): 1095 - 1105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Popova, A. K. Greene, J. Wang, and M. M. Rasenick Phosphatidylinositol 4,5-Bisphosphate Modifies Tubulin Participation in Phospholipase Cbeta 1 Signaling J. Neurosci., March 1, 2002; 22(5): 1668 - 1678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Bennett and A. J. Baines Spectrin and Ankyrin-Based Pathways: Metazoan Inventions for Integrating Cells Into Tissues Physiol Rev, July 1, 2001; 81(3): 1353 - 1392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Berghs, D. Aggujaro, R. Dirkx , Jr., E. Maksimova, P. Stabach, J.-M. Hermel, J.-P. Zhang, W. Philbrick, V. Slepnev, T. Ort, et al. {beta}IV Spectrin, a New Spectrin Localized at Axon Initial Segments and Nodes of Ranvier in the Central and Peripheral Nervous System J. Cell Biol., November 20, 2000; 151(5): 985 - 1002. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hammarlund, W. S. Davis, and E. M. Jorgensen Mutations in {beta}-Spectrin Disrupt Axon Outgrowth and Sarcomere Structure J. Cell Biol., May 15, 2000; 149(4): 931 - 942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. De Matteis and J. Morrow Spectrin tethers and mesh in the biosynthetic pathway J. Cell Sci., January 7, 2000; 113(13): 2331 - 2343. [Abstract] [PDF]
|
|
|
|
|
|
N. Hayes, C Scott, E Heerkens, V Ohanian, A. Maggs, J. Pinder, E Kordeli, and A. Baines Identification of a novel C-terminal variant of beta II spectrin: two isoforms of beta II spectrin have distinct intracellular locations and activities J. Cell Sci., January 6, 2000; 113(11): 2023 - 2034. [Abstract] [PDF]
|
|
|
|
 |
|
 P. Devarajan, P. R. Stabach, M. A. De Matteis, and J. S. Morrow Na,K-ATPase transport from endoplasmic reticulum to Golgi requires the Golgi spectrin-ankyrin G119 skeleton in Madin Darby canine kidney cells PNAS, September 30, 1997; 94(20): 10711 - 10716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yao, H. Suzuki, K. Ozawa, J. Deng, C. Lehel, H. Fukamachi, W. B. Anderson, Y. Kawakami, and T. Kawakami Interactions between Protein Kinase C and Pleckstrin Homology Domains. INHIBITION BY PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE AND PHORBOL 12-MYRISTATE 13-ACETATE J. Biol. Chem., May 16, 1997; 272(20): 13033 - 13039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Auethavekiat, C. S. Abrams, and P. W. Majerus Phosphorylation of Platelet Pleckstrin Activates Inositol Polyphosphate 5-Phosphatase I J. Biol. Chem., January 17, 1997; 272(3): 1786 - 1790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zheng, D. Zangrilli, R. A. Cerione, and A. Eva The Pleckstrin Homology Domain Mediates Transformation by Oncogenic Dbl through Specific Intracellular Targeting J. Biol. Chem., August 9, 1996; 271(32): 19017 - 19020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Adams, T. M. Dwyer, L. L. Dowler, R. A. White, and S. C. Froehner Mouse alpha1- and beta2-Syntrophin Gene Structure, Chromosome Localization, and Homology with a Discs Large Domain J. Biol. Chem., October 27, 1995; 270(43): 25859 - 25865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Hughes and V. Bennett Adducin: a Physical Model with Implications for Function in Assembly of Spectrin-Actin Complexes J. Biol. Chem., August 11, 1995; 270(32): 18990 - 18996. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Whitehead, H. Kirk, C. Tognon, G. Trigo-Gonzalez, and R. Kay Expression Cloning of lfc, a Novel Oncogene with Structural Similarities to Guanine Nucleotide Exchange Factors and to the Regulatory Region of Protein Kinase C J. Biol. Chem., August 4, 1995; 270(31): 18388 - 18395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Touhara, W. J. Koch, B. E. Hawes, and R. J. Lefkowitz Mutational Analysis of the Pleckstrin Homology Domain of the [IMAGE]-Adrenergic Receptor Kinase J. Biol. Chem., July 14, 1995; 270(28): 17000 - 17005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Luttrell, B. E. Hawes, K. Touhara, T. v. Biesen, W. J. Koch, and R. J. Lefkowitz Effect of Cellular Expression of Pleckstrin Homology Domains on G[IMAGE]-coupled Receptor Signaling J. Biol. Chem., June 2, 1995; 270(22): 12984 - 12989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Pitcher, K. Touhara, E. S. Payne, and R. J. Lefkowitz Pleckstrin Homology Domain-mediated Membrane Association and Activation of the [IMAGE]-Adrenergic Receptor Kinase Requires Coordinate Interaction with G[IMAGE] Subunits and Lipid J. Biol. Chem., May 19, 1995; 270(20): 11707 - 11710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Pradhan, C. R. Lombardo, S. Roe, D. L. Rimm, and J. S. Morrow alpha -Catenin Binds Directly to Spectrin and Facilitates Spectrin-Membrane Assembly in Vivo J. Biol. Chem., February 2, 2001; 276(6): 4175 - 4181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. T. Tse, J. Tang, O. Jin, C. Korsgren, K. M. John, A. L. Kung, B. Gwynn, L. L. Peters, and S. E. Lux A New Spectrin, beta IV, Has a Major Truncated Isoform That Associates with Promyelocytic Leukemia Protein Nuclear Bodies and the Nuclear Matrix J. Biol. Chem., June 22, 2001; 276(26): 23974 - 23985. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
