HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Li, Q. Articles by Gardner, D. G. Search for Related Content PubMed PubMed Citation Articles by Li, Q. Articles by Gardner, D. G. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 269, Issue 7, 4934-4939, 02, 1994

Negative regulation of the human atrial natriuretic peptide gene by 1,25-dihydroxyvitamin D3

Q Li and DG Gardner
Metabolic Research Unit, University of California, San Francisco 94143.

We have examined the effect of 1,25-dihydroxyvitamin D3 on the promoter activity of the atrial natriuretic peptide (ANP) gene in cultured neonatal rat atrial myocytes. In acute transfection studies 1,25- dihydroxyvitamin D3 inhibited the expression of a human ANP (hANP) promoter-driven chloramphenicol acetyltransferase reporter (-1150 hANP CAT) in a dose-dependent fashion (10(-10)-10(-8) M). When an expression vector for the vitamin D receptor (VDR) (pSVL-VDR) was introduced together with the reporter plasmid, there was a significant ligand- dependent amplification of the vitamin D-dependent inhibition. Deletion analysis of the 5'-flanking sequence localized the suppressible promoter sequence to within 104 base pairs of the transcription start site of the hANP gene. Thyroid hormone, glucocorticoid, estrogen, and retinoic acid receptor were incapable of mimicking the VDR-dependent inhibition. Retinoid X receptor, on the other hand, effected a significant reduction in hANP promoter activity which was at least additive with that produced by the liganded VDR. The VDR-dependent inhibition displayed promoter selectivity. Both the SV40 promoter and a conventional vitamin D response element linked to a truncated SV40 promoter were activated by the liganded vitamin D receptor, whereas the Rous sarcoma virus promoter was unaffected. On the other hand, the cardiac-specific troponin T promoter was suppressed in a fashion similar to ANP. These findings imply a potentially important role for vitamin D3 in the regulation of gene transcription in myocardial cells.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Chen, D. J. Glenn, W. Ni, C. L. Grigsby, K. Olsen, M. Nishimoto, C. S. Law, and D. G. Gardner Expression of the Vitamin D Receptor Is Increased in the Hypertrophic Heart Hypertension, December 1, 2008; 52(6): 1106 - 1112. [Abstract] [Full Text] [PDF]

				S. Chen, X.-P. Ni, M. H. Humphreys, and D. G. Gardner 1,25 Dihydroxyvitamin D Amplifies Type A Natriuretic Peptide Receptor Expression and Activity in Target Cells J. Am. Soc. Nephrol., February 1, 2005; 16(2): 329 - 339. [Abstract] [Full Text] [PDF]

				E. R. Hines, O. I. Kolek, M. D. Jones, S. H. Serey, N. B. Sirjani, P. R. Kiela, P. W. Jurutka, M. R. Haussler, J. F. Collins, and F. K. Ghishan 1,25-Dihydroxyvitamin D3 Down-regulation of PHEX Gene Expression Is Mediated by Apparent Repression of a 110 kDa Transfactor That Binds to a Polyadenine Element in the Promoter J. Biol. Chem., November 5, 2004; 279(45): 46406 - 46414. [Abstract] [Full Text] [PDF]

				M. L. Clabby, T. A. Robison, H. F. Quigley, D. B. Wilson, and D. P. Kelly Retinoid X Receptor alpha Represses GATA-4-mediated Transcription via a Retinoid-dependent Interaction with the Cardiac-enriched Repressor FOG-2 J. Biol. Chem., February 14, 2003; 278(8): 5760 - 5767. [Abstract] [Full Text] [PDF]

				B. C Kone Molecular biology of natriuretic peptides and nitric oxide synthases Cardiovasc Res, August 15, 2001; 51(3): 429 - 441. [Abstract] [Full Text] [PDF]

				S. Chen, J. Cui, K. Nakamura, R. C. J. Ribeiro, B. L. West, and D. G. Gardner Coactivator-Vitamin D Receptor Interactions Mediate Inhibition of the Atrial Natriuretic Peptide Promoter J. Biol. Chem., May 12, 2000; 275(20): 15039 - 15048. [Abstract] [Full Text] [PDF]

				T. L. Towers, T. P. Staeva, and L. P. Freedman A Two-Hit Mechanism for Vitamin D3-Mediated Transcriptional Repression of the Granulocyte-Macrophage Colony-Stimulating Factor Gene: Vitamin D Receptor Competes for DNA Binding with NFAT1 and Stabilizes c-Jun Mol. Cell. Biol., June 1, 1999; 19(6): 4191 - 4199. [Abstract] [Full Text] [PDF]

				S. Chen, C. H. R. M. Costa, K. Nakamura, R. C. J. Ribeiro, and D. G. Gardner Vitamin D-dependent Suppression of Human Atrial Natriuretic Peptide Gene Promoter Activity Requires Heterodimer Assembly J. Biol. Chem., April 16, 1999; 274(16): 11260 - 11266. [Abstract] [Full Text] [PDF]

				N. J. Koszewski, S. Ashok, and J. Russell Turning a Negative into a Positive: Vitamin D Receptor Interactions with the Avian Parathyroid Hormone Response Element Mol. Endocrinol., March 1, 1999; 13(3): 455 - 465. [Abstract] [Full Text]

				M. L Kuroski de Bold Estrogen, natriuretic peptides and the renin-angiotensin system Cardiovasc Res, March 1, 1999; 41(3): 524 - 531. [Abstract] [Full Text] [PDF]

				G. F. Wang, W. Nikovits Jr., M. Schleinitz, and F. E. Stockdale A Positive GATA Element and a Negative Vitamin D Receptor-Like Element Control Atrial Chamber-Specific Expression of a Slow Myosin Heavy-Chain Gene during Cardiac Morphogenesis Mol. Cell. Biol., October 1, 1998; 18(10): 6023 - 6034. [Abstract] [Full Text]

				G. JONES, S. A. STRUGNELL, and H. F. DeLUCA Current Understanding of the Molecular Actions of Vitamin D Physiol Rev, October 1, 1998; 78(4): 1193 - 1231. [Abstract] [Full Text] [PDF]

				T. W. Kurtz and D. G. Gardner Transcription-Modulating Drugs : A New Frontier in the Treatment of Essential Hypertension Hypertension, September 1, 1998; 32(3): 380 - 386. [Abstract] [Full Text] [PDF]

				S. Chen, J. Wu, J.-C. Hsieh, G. K. Whitfield, P. W. Jurutka, M. R. Haussler, and D. G. Gardner Suppression of ANP Gene Transcription by Liganded Vitamin D Receptor : Involvement of Specific Receptor Domains Hypertension, June 1, 1998; 31(6): 1338 - 1342. [Abstract] [Full Text] [PDF]

				T. L. Towers and L. P. Freedman Granulocyte-Macrophage Colony-stimulating Factor Gene Transcription Is Directly Repressed by the Vitamin D3 Receptor. IMPLICATIONS FOR ALLOSTERIC INFLUENCES ON NUCLEAR RECEPTOR STRUCTURE AND FUNCTION BY A DNA ELEMENT J. Biol. Chem., April 24, 1998; 273(17): 10338 - 10348. [Abstract] [Full Text] [PDF]

				J. G. Meszaros, N. J. Karin, K. Akanbi, and M. C. Farach-Carson Down-regulation of L-type Ca2+ Channel Transcript Levels by 1,25-Dihyroxyvitamin D3. OSTEOBLASTIC CELLS EXPRESS L-TYPE alpha 1C Ca2+ CHANNEL ISOFORMS J. Biol. Chem., December 20, 1996; 271(51): 32981 - 32985. [Abstract] [Full Text] [PDF]

				G. F. Wang, W. Nikovits Jr., M. Schleinitz, and F. E. Stockdale Atrial Chamber-specific Expression of the Slow Myosin Heavy Chain 3Gene in the Embryonic Heart J. Biol. Chem., August 16, 1996; 271(33): 19836 - 19845. [Abstract] [Full Text] [PDF]