|
|
|
|||||||
|
|||||||||
(Received for publication, August 2, 1994; and in revised form, October 28, 1994 ) Copper-zinc superoxide dismutase (SOD-1) is an enzyme that is
widely expressed in eukaryotic cells and performs a vital role in
protecting cells against free radical damage. In mouse testis, three
different sizes of SOD-1 mRNAs of about 0.73, 0.80, and 0.93 kilobases
(kb) are detected. The 0.73-kb mRNA is found in early stages of male
germ cells and in all somatic tissues. The mRNAs of 0.80 and 0.93 kb
are exclusively detected in post-meiotic germ cells. RNase H digestions
and Northern blot analyses reveal that the three SOD-1 mRNAs are
derived from two transcripts, a ubiquitously expressed transcript and a
post-meiotic transcript, which differ by 114-120 nucleotides.
RNase protection assays demonstrate that the additional nucleotides
present in the post-meiotic mRNA are solely in the 5`-untranslated
region. Using a probe derived from the 5`-untranslated region of the
0.93-kb SOD-1 mRNA, we have established that it originates from an
alternative upstream promoter contiguous with the somatic SOD-1
promoter. Polysomal gradient analysis of the three mouse testis SOD-1
mRNAs reveals that the 0.93-kb SOD-1 mRNA is primarily non-polysomal,
while the 0.80- and 0.73-kb SOD-1 mRNAs are mostly polysome associated.
A faster migrating form of the 0.93-kb SOD-1 mRNA is present on
polysomes as a result of partial deadenylation. In a cell-free
translation system, the 0.73-kb SOD-1 mRNA translates about 2-fold more
efficiently than the 0.93-kb SOD-1 mRNA. These data demonstrate that
male germ cells transcribe two size classes of SOD-1 mRNAs with
different translation potential by utilizing two different promoters,
post-meiotic SOD-1 mRNAs undergo adenylation changes, and one of the
post-meiotic SOD-1 mRNAs is transcribed during mid-spermiogenesis and
translated days later in a partially deadenylated form.
Volume 270,
Number 1,
Issue of January 6, 1995 pp. 236-243
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  W. Gu, A. L. Wells, F. Pan, and R. H. Singer Feedback Regulation between Zipcode Binding Protein 1 and {beta}-Catenin mRNAs in Breast Cancer Cells Mol. Cell. Biol., August 15, 2008; 28(16): 4963 - 4974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Obermann, I. Raabe, M. Balvers, B. Brunswig, W. Schulze, and C. Kirchhoff Novel testis-expressed profilin IV associated with acrosome biogenesis and spermatid elongation Mol. Hum. Reprod., January 1, 2005; 11(1): 53 - 64. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Otulakowski, B. Rafii, H. R. Bremner, and H. O'Brodovich Structure and Hormone Responsiveness of the Gene Encoding the alpha -Subunit of the Rat Amiloride-Sensitive Epithelial Sodium Channel Am. J. Respir. Cell Mol. Biol., May 1, 1999; 20(5): 1028 - 1040. [Abstract] [Full Text]
|
|
|
|
|
|
L. O. Goodwin, N. B. Leeds, D. Guzowski, I. R. Hurley, R. G. Pergolizzi, and S. Benoff Identification of structural elements of the testis-specific voltage dependent calcium channel that potentially regulate its biophysical properties Mol. Hum. Reprod., April 1, 1999; 5(4): 311 - 322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Mezquita, C. Mezquita, and J. Mezquita Novel transcripts of carbonic anhydrase II in mouse and human testis Mol. Hum. Reprod., March 1, 1999; 5(3): 199 - 205. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |