In this report we have analyzed the binding of collagen to Streptococcus pyogenes strain 6414. This binding was rapid, specific, and involved a limited number of receptor molecules (11,600 copies per cell). When the proteins in a streptococcal lysate were blotted onto a nitrocellulose filter and probed with I-labeled collagen, a prominent collagen-binding protein of 57 kDa was identified as well as minor 130-150-kDa components. The major 57-kDa protein was isolated by affinity chromatography on collagen-Sepharose followed by gel filtration chromatography. The 57-kDa protein purified from S. pyogenes was used to raise a monospecific antibody which also reacted with a collagen-binding protein of similar molecular size isolated from Streptococcus zooepidemicus. The two collagen-binding proteins from streptococci have a similar amino acid composition and isoelectric points. Isolated collagen-binding protein was specifically recognized by I-collagen in a solid-phase binding assay and displayed an affinity for the ligand quite similar to that exhibited by intact bacteria (K= 3.1 versus 3.5 10M, respectively). Surface-labeled bacteria attached to microtiter wells coated with different collagen types and the 57-kDa protein blocked the adhesion to collagen substrate. We propose that the 57-kDa protein is an adhesin involved in the attachment of streptococci to host tissues.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				B. Kreikemeyer, M. Nakata, S. Oehmcke, C. Gschwendtner, J. Normann, and A. Podbielski Streptococcus pyogenes Collagen Type I-binding Cpa Surface Protein: EXPRESSION PROFILE, BINDING CHARACTERISTICS, BIOLOGICAL FUNCTIONS, AND POTENTIAL CLINICAL IMPACT J. Biol. Chem., September 30, 2005; 280(39): 33228 - 33239. [Abstract] [Full Text] [PDF]

				R.M. Love and H.F. Jenkinson INVASION OF DENTINAL TUBULES BY ORAL BACTERIA Critical Reviews in Oral Biology & Medicine, March 1, 2002; 13(2): 171 - 183. [Abstract] [Full Text] [PDF]

				M. W. Cunningham Pathogenesis of Group A Streptococcal Infections Clin. Microbiol. Rev., July 1, 2000; 13(3): 470 - 511. [Abstract] [Full Text] [PDF]

				A. Passi, D. Negrini, R. Albertini, G. De Luca, and G. Miserocchi Involvement of lung interstitial proteoglycans in development of hydraulic- and elastase-induced edema Am J Physiol Lung Cell Mol Physiol, September 1, 1998; 275(3): L631 - L635. [Abstract] [Full Text] [PDF]

				D. Negrini, A. Passi, G. De Luca, and G. Miserocchi Proteoglycan involvement during development of lesional pulmonary edema Am J Physiol Lung Cell Mol Physiol, February 1, 1998; 274(2): L203 - L211. [Abstract] [Full Text] [PDF]

				H.F. Jenkinson and R. Lamont Streptococcal Adhesion and Colonization Critical Reviews in Oral Biology & Medicine, January 1, 1997; 8(2): 175 - 200. [Abstract] [Full Text] [PDF]

				L. Visai, Y. Xu, F. Casolini, S. Rindi, M. Hook, and P. Speziale Monoclonal Antibodies to CNA, a Collagen-binding Microbial Surface Component Recognizing Adhesive Matrix Molecules, Detach Staphylococcus aureus from a Collagen Substrate J. Biol. Chem., December 15, 2000; 275(51): 39837 - 39845. [Abstract] [Full Text] [PDF]