A new protein tyrosine phosphatase (PC12-PTP1) was identified in nerve growth factor (NGF)-treated PC12 cells. The mRNA level of PC12-PTP1 is increased 9-fold over the initial 8 h of NGF treatment and then decreases dramatically after 24 h of treatment. In rat brain, three transcripts corresponding to 1.5, 2.6, and 3.0 kilobases (kb) in size are detected by Northern blot analysis. Although the 1.5- and 2.6-kb transcripts are present in brain and other tissues, the 3-kb transcript is exclusively expressed in brain and the expression of this transcript alone increases following NGF treatment. PC12-PTP1 is a non-receptor protein tyrosine phosphatase (PTP) with a 50% sequence homology in the phosphatase domain with several other non-receptor PTPs. PC12-PTP1 fusion protein exhibits tyrosine phosphatase activity, and in vitro translation of the PC12-PTP1 transcript produces a major protein of 39 kDa. The data presented suggest that NGF regulates the expression of PC12-PTP1 during periods of neuronal growth and differentiation.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				J.R.A. Sherwin, A.M. Sharkey, A. Mihalyi, P. Simsa, R.D. Catalano, and T.M. D'Hooghe Global gene analysis of late secretory phase, eutopic endometrium does not provide the basis for a minimally invasive test of endometriosis Hum. Reprod., May 1, 2008; 23(5): 1063 - 1068. [Abstract] [Full Text] [PDF]

				J. Cottom, L. M. Salvador, E. T. Maizels, S. Reierstad, Y. Park, D. W. Carr, M. A. Davare, J. W. Hell, S. S. Palmer, P. Dent, et al. Follicle-stimulating Hormone Activates Extracellular Signal-regulated Kinase but Not Extracellular Signal-regulated Kinase Kinase through a 100-kDa Phosphotyrosine Phosphatase J. Biol. Chem., February 21, 2003; 278(9): 7167 - 7179. [Abstract] [Full Text] [PDF]

				M. Buschbeck, J. Eickhoff, M. N. Sommer, and A. Ullrich Phosphotyrosine-specific Phosphatase PTP-SL Regulates the ERK5 Signaling Pathway J. Biol. Chem., August 9, 2002; 277(33): 29503 - 29509. [Abstract] [Full Text] [PDF]

				C. Blanco-Aparicio, J. Torres, and R. Pulido A Novel Regulatory Mechanism of MAP Kinases Activation and Nuclear Translocation Mediated by PKA and the PTP-SL Tyrosine Phosphatase J. Cell Biol., December 13, 1999; 147(6): 1129 - 1136. [Abstract] [Full Text] [PDF]

				M. Oh-hora, M. Ogata, Y. Mori, M. Adachi, K. Imai, A. Kosugi, and T. Hamaoka Direct Suppression of TCR-Mediated Activation of Extracellular Signal-Regulated Kinase by Leukocyte Protein Tyrosine Phosphatase, a Tyrosine-Specific Phosphatase J. Immunol., August 1, 1999; 163(3): 1282 - 1288. [Abstract] [Full Text] [PDF]

				M. Saxena, S. Williams, J. Brockdorff, J. Gilman, and T. Mustelin Inhibition of T Cell Signaling by Mitogen-activated Protein Kinase-targeted Hematopoietic Tyrosine Phosphatase (HePTP) J. Biol. Chem., April 23, 1999; 274(17): 11693 - 11700. [Abstract] [Full Text] [PDF]

				M.-C. Wang, J. H. Liu, and F.-F. Wang Protein Tyrosine Phosphatase-Dependent Activation of beta -Globin and delta -Aminolevulinic Acid Synthase Genes in the Camptothecin-Induced IW32 Erythroleukemia Cell Differentiation Mol. Pharmacol., April 1, 1997; 51(4): 558 - 566. [Abstract] [Full Text]

				N. Aoki, Y. Yamaguchi-Aoki, and A. Ullrich The Novel Protein-tyrosine Phosphatase PTP20 Is a Positive Regulator of PC12 Cell Neuronal Differentiation J. Biol. Chem., November 15, 1996; 271(46): 29422 - 29426. [Abstract] [Full Text] [PDF]

				M. Chiang and J. Flanagan PTP-NP, a new member of the receptor protein tyrosine phosphatase family, implicated in development of nervous system and pancreatic endocrine cells Development, July 1, 1996; 122(7): 2239 - 2250. [Abstract] [PDF]