Genetic studies were performed to examine the role of eukaryotic dnaJ protein, Ydj1p, in the regulated activation of human androgen receptor (hAR) after heterologous expression in Saccharomyces cerevisiae. Hormone-dependent activation of hAR was measured as a function of lacZ reporter gene expression, which was defective in ydj1-151 and ydj1-2 null mutant strains compared to the wild type. This defect was not due to receptor misfolding, since hAR in both wild type and mutant strains had a similar capacity to bind hormone. The target for Ydj1p action was determined to be the hAR hormone binding domain since an N-terminal fragment lacking this region was constitutively active in both wild type and ydj1-151 mutant strains. These data correlate hormone dependence of hAR activation with a requirement for Ydj1p function and are consistent with a role for dnaJ proteins in signal transduction by steroid hormone receptors.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				C. M. Wright, S. W. Fewell, M. L. Sullivan, J. M. Pipas, S. C. Watkins, and J. L. Brodsky The Hsp40 Molecular Chaperone Ydj1p, Along With the Protein Kinase C Pathway, Affects Cell-Wall Integrity in the Yeast Saccharomyces cerevisiae Genetics, April 1, 2007; 175(4): 1649 - 1664. [Abstract] [Full Text] [PDF]

				C.-Y. Fan, H.-Y. Ren, P. Lee, A. J. Caplan, and D. M. Cyr The Type I Hsp40 Zinc Finger-like Region Is Required for Hsp70 to Capture Non-native Polypeptides from Ydj1 J. Biol. Chem., January 7, 2005; 280(1): 695 - 702. [Abstract] [Full Text] [PDF]

				C. Lan, H. C. Lee, S. Tang, and L. Zhang A Novel Mode of Chaperone Action: HEME ACTIVATION OF Hap1 BY ENHANCED ASSOCIATION OF Hsp90 WITH THE REPRESSED Hsp70-Hap1 COMPLEX J. Biol. Chem., June 25, 2004; 279(26): 27607 - 27612. [Abstract] [Full Text] [PDF]

				L. F. Agnati, S. Ferre, C. Lluis, R. Franco, and K. Fuxe Molecular Mechanisms and Therapeutical Implications of Intramembrane Receptor/Receptor Interactions among Heptahelical Receptors with Examples from the Striatopallidal GABA Neurons Pharmacol. Rev., September 1, 2003; 55(3): 509 - 550. [Abstract] [Full Text] [PDF]

				W. B. Pratt and D. O. Toft Regulation of Signaling Protein Function and Trafficking by the hsp90/hsp70-Based Chaperone Machinery Experimental Biology and Medicine, February 1, 2003; 228(2): 111 - 133. [Abstract] [Full Text] [PDF]

				P. Lee, J. Rao, A. Fliss, E. Yang, S. Garrett, and A. J. Caplan The Cdc37 protein kinase-binding domain is sufficient for protein kinase activity and cell viability J. Cell Biol., December 23, 2002; 159(6): 1051 - 1059. [Abstract] [Full Text] [PDF]

				J. L. Goeckeler, A. Stephens, P. Lee, A. J. Caplan, and J. L. Brodsky Overexpression of Yeast Hsp110 Homolog Sse1p Suppresses ydj1-151 Thermosensitivity and Restores Hsp90-dependent Activity Mol. Biol. Cell, August 1, 2002; 13(8): 2760 - 2770. [Abstract] [Full Text] [PDF]

				E. A. A. Nollen and R. I. Morimoto Chaperoning signaling pathways: molecular chaperones as stress-sensing `heat shock' proteins J. Cell Sci., July 15, 2002; 115(14): 2809 - 2816. [Abstract] [Full Text] [PDF]

				C. A. Heinlein and C. Chang Androgen Receptor (AR) Coregulators: An Overview Endocr. Rev., April 1, 2002; 23(2): 175 - 200. [Abstract] [Full Text] [PDF]

				M. P. Hernandez, A. Chadli, and D. O. Toft HSP40 Binding Is the First Step in the HSP90 Chaperoning Pathway for the Progesterone Receptor J. Biol. Chem., March 29, 2002; 277(14): 11873 - 11881. [Abstract] [Full Text] [PDF]

				B. He, J. T. Minges, L. W. Lee, and E. M. Wilson The FXXLF Motif Mediates Androgen Receptor-specific Interactions with Coregulators J. Biol. Chem., March 15, 2002; 277(12): 10226 - 10235. [Abstract] [Full Text] [PDF]

				V. Shridhar, K. C. Bible, J. Staub, R. Avula, Y. K. Lee, K. Kalli, H. Huang, L. C. Hartmann, S. H. Kaufmann, and D. I. Smith Loss of Expression of a New Member of the DNAJ Protein Family Confers Resistance to Chemotherapeutic Agents Used in the Treatment of Ovarian Cancer Cancer Res., May 1, 2001; 61(10): 4258 - 4265. [Abstract] [Full Text]

				S. Sarrió, V. Casadó, M. Escriche, F. Ciruela, J. Mallol, E. I. Canela, C. Lluis, and R. Franco The Heat Shock Cognate Protein hsc73 Assembles with A1 Adenosine Receptors To Form Functional Modules in the Cell Membrane Mol. Cell. Biol., July 15, 2000; 20(14): 5164 - 5174. [Abstract] [Full Text]

				J. L. Johnson and E. A. Craig A Role for the Hsp40 Ydj1 in Repression of Basal Steroid Receptor Activity in Yeast Mol. Cell. Biol., May 1, 2000; 20(9): 3027 - 3036. [Abstract] [Full Text]

				B. C. Freeman, S. J. Felts, D. O. Toft, and K. R. Yamamoto The p23 molecular chaperones act at a late step in intracellular receptor action to differentially affect ligand efficacies Genes & Dev., February 15, 2000; 14(4): 422 - 434. [Abstract] [Full Text]

				A. E. Fliss, J. Rao, M. W. Melville, M. E. Cheetham, and A. J. Caplan Domain Requirements of DnaJ-like (Hsp40) Molecular Chaperones in the Activation of a Steroid Hormone Receptor J. Biol. Chem., November 26, 1999; 274(48): 34045 - 34052. [Abstract] [Full Text] [PDF]

				G. C. Meacham, B. L. Browne, W. Zhang, R. Kellermayer, D. M. Bedwell, and D. M. Cyr Mutations in the Yeast Hsp40 Chaperone Protein Ydj1 Cause Defects in Axl1 Biogenesis and Pro-a-factor Processing J. Biol. Chem., November 26, 1999; 274(48): 34396 - 34402. [Abstract] [Full Text] [PDF]

				D. F. Nathan, M. H. Vos, and S. Lindquist Identification of SSF1, CNS1, and HCH1 as multicopy suppressors of a Saccharomyces cerevisiae Hsp90 loss-of-function mutation PNAS, February 16, 1999; 96(4): 1409 - 1414. [Abstract] [Full Text] [PDF]

				H. Miyamoto, S. Yeh, H. Lardy, E. Messing, and C. Chang Delta 5-Androstenediol is a natural hormone with androgenic activity in human prostate cancer cells PNAS, September 15, 1998; 95(19): 11083 - 11088. [Abstract] [Full Text] [PDF]

				Y. Fang, A. E. Fliss, J. Rao, and A. J. Caplan SBA1 Encodes a Yeast Hsp90 Cochaperone That Is Homologous to Vertebrate p23 Proteins Mol. Cell. Biol., July 1, 1998; 18(7): 3727 - 3734. [Abstract] [Full Text]

				L. Zhang, A. Hach, and C. Wang Molecular Mechanism Governing Heme Signaling in Yeast: a Higher-Order Complex Mediates Heme Regulation of the Transcriptional Activator HAP1 Mol. Cell. Biol., July 1, 1998; 18(7): 3819 - 3828. [Abstract] [Full Text]

				K. D. Dittmar, M. Banach, M. D. Galigniana, and W. B. Pratt The Role of DnaJ-like Proteins in Glucocorticoid Receptor·hsp90 Heterocomplex Assembly by the Reconstituted hsp90·p60·hsp70 Foldosome Complex J. Biol. Chem., March 27, 1998; 273(13): 7358 - 7366. [Abstract] [Full Text] [PDF]

				Z. Lu and D. M. Cyr The Conserved Carboxyl Terminus and Zinc Finger-like Domain of the Co-chaperone Ydj1 Assist Hsp70 in Protein Folding J. Biol. Chem., March 6, 1998; 273(10): 5970 - 5978. [Abstract] [Full Text] [PDF]

				K. Terada, M. Kanazawa, B. Bukau, and M. Mori The Human DnaJ Homologue dj2 Facilitates Mitochondrial Protein Import and Luciferase Refolding J. Cell Biol., December 1, 1997; 139(5): 1089 - 1095. [Abstract] [Full Text] [PDF]

				A. E. Fliss, Y. Fang, F. Boschelli, and A. J. Caplan Differential In Vivo Regulation of Steroid Hormone Receptor Activation by Cdc37p Mol. Biol. Cell, December 1, 1997; 8(12): 2501 - 2509. [Abstract] [Full Text]

				W. B. Pratt and D. O. Toft Steroid Receptor Interactions with Heat Shock Protein and Immunophilin Chaperones Endocr. Rev., June 1, 1997; 18(3): 306 - 360. [Abstract] [Full Text]

				K. D. Dittmar and W. B. Pratt Folding of the Glucocorticoid Receptor by the Reconstituted hsp90-based Chaperone Machinery. THE INITIAL hsp90·p60·hsp70-DEPENDENT STEP IS SUFFICIENT FOR CREATING THE STEROID BINDING CONFORMATION J. Biol. Chem., May 16, 1997; 272(20): 13047 - 13054. [Abstract] [Full Text] [PDF]

				W. Bai, B. G. Rowan, V. E. Allgood, B. W. O'Malley, and N. L. Weigel Differential Phosphorylation of Chicken Progesterone Receptor in Hormone-dependent and Ligand-independent Activation J. Biol. Chem., April 18, 1997; 272(16): 10457 - 10463. [Abstract] [Full Text] [PDF]

				A. R. Schutz, T. H. Giddings Jr., E. Steiner, and M. Winey The Yeast CDC37 Gene Interacts with MPS1 and Is Required for Proper Execution of Spindle Pole Body Duplication J. Cell Biol., March 10, 1997; 136(5): 969 - 982. [Abstract] [Full Text] [PDF]

				H. Irmer and J. Hohfeld Characterization of Functional Domains of the Eukaryotic Co-chaperone Hip J. Biol. Chem., January 24, 1997; 272(4): 2230 - 2235. [Abstract] [Full Text] [PDF]

				M. W. Melville, W. J. Hansen, B. C. Freeman, W. J. Welch, and M. G. Katze The molecular chaperone hsp40 regulates the activity of P58IPK, the cellular inhibitor of PKR PNAS, January 7, 1997; 94(1): 97 - 102. [Abstract] [Full Text] [PDF]

				Y. Fang, A. E. Fliss, D. M. Robins, and A. J. Caplan Hsp90 Regulates Androgen Receptor Hormone Binding Affinity in Vivo J. Biol. Chem., November 8, 1996; 271(45): 28697 - 28702. [Abstract] [Full Text] [PDF]

				T. Ratajczak and A. Carrello Cyclophilin 40 (CyP-40), Mapping of Its hsp90 Binding Domain and Evidence That FKBP52 Competes with CyP-40 for hsp90 Binding J. Biol. Chem., February 9, 1996; 271(6): 2961 - 2965. [Abstract] [Full Text] [PDF]

				E. Langley, Z.-x. Zhou, and E. M. Wilson Evidence for an Anti-parallel Orientation of the Ligand-activated Human Androgen Receptor Dimer J. Biol. Chem., December 15, 1995; 270(50): 29983 - 29990. [Abstract] [Full Text] [PDF]

				S. P. Bohen and S. P. Bohen Hsp90 Mutants Disrupt Glucocorticoid Receptor Ligand Binding and Destabilize Aporeceptor Complexes J. Biol. Chem., December 8, 1995; 270(49): 29433 - 29438. [Abstract] [Full Text] [PDF]

				P. Coumailleau, L. Poellinger, J.Åk. Gustafsson, and M. L. Whitelaw Definition of a Minimal Domain of the Dioxin Receptor That Is Associated with Hsp90 and Maintains Wild Type Ligand Binding Affinity and Specificity J. Biol. Chem., October 20, 1995; 270(42): 25291 - 25300. [Abstract] [Full Text] [PDF]

				S. Bohen, A Kralli, and K. Yamamoto Hold 'em and fold 'em: chaperones and signal transduction Science, June 2, 1995; 268(5215): 1303 - 1304. [PDF]

				J. Rao, P. Lee, S. Benzeno, C. Cardozo, J. Albertus, D. M. Robins, and A. J. Caplan Functional Interaction of Human Cdc37 with the Androgen Receptor but Not with the Glucocorticoid Receptor J. Biol. Chem., February 16, 2001; 276(8): 5814 - 5820. [Abstract] [Full Text] [PDF]