| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, September 27, 1994; and in revised form, December 1,
1994) Deletion of the amino-terminal domain of Raf-1, which contains
the Ras-binding region, results in the constitutive activation of the
liberated Raf-1 catalytic domain in fibroblast cell lines. We
demonstrate that the MEK kinase activity of the isolated Raf-1
catalytic domain, Raf-BXB, is not constitutively active, but is
regulated in Jurkat T cells. Raf-BXB is activated by engaging the
antigen receptor-CD3 complex, or treating cells with phorbol myristate
acetate or okadaic acid. Increasing intracellular cAMP inhibits Raf-1
activation stimulated by phorbol myristate acetate, but not the
activation of Raf-BXB. Serine 621, but not serine 499, is essential for
Raf-BXB MEK kinase activity. Because Raf-BXB does not bind Ras, the
data establishes a Ras-independent signal in directly regulating the
activity of the Raf-1 catalytic domain.
Volume 270,
Number 10,
Issue of March 10, 1995 pp. 5594-5599
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Balmanno, T. Millar, M. McMahon, and S. J. Cook {Delta}Raf-1:ER* Bypasses the Cyclic AMP Block of Extracellular Signal-Regulated Kinase 1 and 2 Activation but Not CDK2 Activation or Cell Cycle Reentry Mol. Cell. Biol., December 15, 2003; 23(24): 9303 - 9317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Xiang, M. Zang, C. A. Waelde, R. Wen, and Z. Luo Phosphorylation of 338SSYY341 Regulates Specific Interaction between Raf-1 and MEK1 J. Biol. Chem., November 15, 2002; 277(47): 44996 - 45003. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Shan, R. Balakir, G. Criado, J. S. Wood, M.-C. Seminario, J. Madrenas, and R. L. Wange ZAP-70-Independent Ca2+ Mobilization and Erk Activation in Jurkat T Cells in Response to T-Cell Antigen Receptor Ligation Mol. Cell. Biol., November 1, 2001; 21(21): 7137 - 7149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Banfi, P. Eriksson, G. Giandomenico, L. Mussoni, L. Sironi, A. Hamsten, and E. Tremoli Transcriptional Regulation of Plasminogen Activator Inhibitor Type 1 Gene by Insulin: Insights Into the Signaling Pathway Diabetes, July 1, 2001; 50(7): 1522 - 1530. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Pearson, F. Robinson, T. Beers Gibson, B.-e Xu, M. Karandikar, K. Berman, and M. H. Cobb Mitogen-Activated Protein (MAP) Kinase Pathways: Regulation and Physiological Functions Endocr. Rev., April 1, 2001; 22(2): 153 - 183. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. G. Puente, J. C. Stone, and H. L. Ostergaard Evidence for Protein Kinase C-Dependent and -Independent Activation of Mitogen-Activated Protein Kinase in T Cells: Potential Role of Additional Diacylglycerol Binding Proteins J. Immunol., December 15, 2000; 165(12): 6865 - 6871. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Kerkhoff, L. M. Fedorov, R. Siefken, A. O. Walter, T. Papadopoulos, and U. R. Rapp Lung-targeted Expression of the c-Raf-1 Kinase in Transgenic Mice Exposes a Novel Oncogenic Character of the Wild-Type Protein Cell Growth Differ., April 1, 2000; 11(4): 185 - 190. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Hartkamp, J. Troppmair, and U. R. Rapp The JNK/SAPK Activator Mixed Lineage Kinase 3 (MLK3) Transforms NIH 3T3 Cells in a MEK-dependent Fashion Cancer Res., May 1, 1999; 59(9): 2195 - 2202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Laird, D. K. Morrison, and D. Shalloway Characterization of Raf-1 Activation in Mitosis J. Biol. Chem., February 12, 1999; 274(7): 4430 - 4439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Suhasini, H. Li, S. M. Lohmann, G. R. Boss, and R. B. Pilz Cyclic-GMP-Dependent Protein Kinase Inhibits the Ras/Mitogen-Activated Protein Kinase Pathway Mol. Cell. Biol., December 1, 1998; 18(12): 6983 - 6994. [Abstract] [Full Text]
|
|
|
|
|
|
J. A. Thorson, L. W. K. Yu, A. L. Hsu, N.-Y. Shih, P. R. Graves, J. W. Tanner, P. M. Allen, H. Piwnica-Worms, and A. S. Shaw 14-3-3 Proteins Are Required for Maintenance of Raf-1 Phosphorylation and Kinase Activity Mol. Cell. Biol., September 1, 1998; 18(9): 5229 - 5238. [Abstract] [Full Text]
|
|
|
|
|
|
A. Wagle, S. Jivraj, G. L. Garlock, and S. R. Stapleton Insulin Regulation of Glucose-6-phosphate Dehydrogenase Gene Expression Is Rapamycin-sensitive and Requires Phosphatidylinositol 3-Kinase J. Biol. Chem., June 12, 1998; 273(24): 14968 - 14974. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hoffmeyer, A. Avots, E. Flory, C. K. Weber, E. Serfling, and U. R. Rapp The GABP-responsive Element of the Interleukin-2 Enhancer Is Regulated by JNK/SAPK-activating Pathways in T Lymphocytes J. Biol. Chem., April 24, 1998; 273(17): 10112 - 10119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Flory, C. K. Weber, P. Chen, A. Hoffmeyer, C. Jassoy, and U. R. Rapp Plasma Membrane-Targeted Raf Kinase Activates NF-kappa B and Human Immunodeficiency Virus Type 1 Replication in T Lymphocytes J. Virol., April 1, 1998; 72(4): 2788 - 2794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tian, R. D. Smith, T. Balla, and K. J. Catt Angiotensin II Activates Mitogen-Activated Protein Kinase Via Protein Kinase C and Ras/Raf-1 Kinase in Bovine Adrenal Glomerulosa Cells Endocrinology, April 1, 1998; 139(4): 1801 - 1809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Mineo, R. G.W. Anderson, and M. A. White Physical Association with Ras Enhances Activation of Membrane-bound Raf (RafCAAX) J. Biol. Chem., April 18, 1997; 272(16): 10345 - 10348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Adams, M. J. Reginato, D. Shao, M. A. Lazar, and V. K. Chatterjee Transcriptional Activation by Peroxisome Proliferator-activated Receptor gamma Is Inhibited by Phosphorylation at a Consensus Mitogen-activated Protein Kinase Site J. Biol. Chem., February 21, 1997; 272(8): 5128 - 5132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cheng, T. G. Boulton, and M. H. Cobb ERK3 Is a Constitutively Nuclear Protein Kinase J. Biol. Chem., April 12, 1996; 271(15): 8951 - 8958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Gabbay, C. Sutherland, L. Gnudi, B. B. Kahn, R. M. O'Brien, D. K. Granner, and J. S. Flier Insulin Regulation of Phosphoenolpyruvate Carboxykinase Gene Expression Does Not Require Activation of the Ras/Mitogen-activated Protein Kinase Signaling Pathway J. Biol. Chem., January 26, 1996; 271(4): 1890 - 1897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. English, C. A. Vanderbilt, S. Xu, S. Marcus, and M. H. Cobb Isolation of MEK5 and Differential Expression of Alternatively Spliced Forms J. Biol. Chem., December 1, 1995; 270(48): 28897 - 28902. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P Dent, T Jelinek, D. Morrison, M. Weber, and T. Sturgill Reversal of Raf-1 activation by purified and membrane-associated protein phosphatases Science, June 30, 1995; 268(5219): 1902 - 1906. [Abstract] [PDF]
|
|
|
|
|
|
M. H. Cobb and E. J. Goldsmith How MAP Kinases Are Regulated J. Biol. Chem., June 23, 1995; 270(25): 14843 - 14846. [Full Text] [PDF]
|
|
|
|
|
|
M. F. Sidovar, P. Kozlowski, J. W. Lee, M. A. Collins, Y. He, and L. M. Graves Phosphorylation of Serine 43 Is Not Required for Inhibition of c-Raf Kinase by the cAMP-dependent Protein Kinase J. Biol. Chem., September 8, 2000; 275(37): 28688 - 28694. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
