Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mendez, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mendez, A. J.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 270, Number 11, Issue of March 17, 1995 pp. 5891-5900
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
Monensin and Brefeldin A Inhibit High Density Lipoprotein- mediated Cholesterol Efflux from Cholesterol-enriched Cells
IMPLICATIONS FOR INTRACELLULAR CHOLESTEROL TRANSPORT

(Received for publication, October 13, 1994; and in revised form, January 5, 1995)

Armando J. Mendez

Mechanisms and pathways of excess cholesterol removal from intracellular sites of accumulation to extracellular cholesterol acceptors remain poorly defined. To gain further insights, compounds known to affect cellular protein transport pathways were tested for their effects on high density lipoprotein (HDL)-mediated cholesterol efflux from cultured cells enriched with cholesterol. Monensin, nigericin, and brefeldin A inhibited the ability of HDL to decrease cellular cholesterol esterification, stimulate sterol biosynthesis, and promote the efflux of labeled cholesterol and cholesterol mass from fibroblasts and smooth muscle cells. HDL-mediated decrease in cell cholesterol esterification was inhibited up to 80% by these compounds compared with control incubations over an HDL concentration of 5-100 µg/ml and up to 18 h of incubation. Up-regulation of sterol biosynthesis after depletion of cell cholesterol by HDL increased over 10-fold; however, inclusion of monensin or brefeldin A during the incubation completely prevented the increase of sterol biosynthesis by HDL. Efflux of [^3H]cholesterol to HDL from prelabeled cells was inhibited up to 40% by these compounds, and this effect persisted when cholesterol esterification was blocked. Similarly, monensin and brefeldin A inhibited up to 50% of HDL-mediated cholesterol mass efflux relative to controls. Treatment of cells with cholesterol oxidase demonstrated an increase of intracellular cholesterol after exposure to monensin or nigericin and to a lesser extent with brefeldin A. These data show that monensin, nigericin, and brefeldin A sequester cholesterol from sites normally available for efflux by HDL. Since these compounds act by disruption of Golgi complex structure and function, a role for this intracellular organelle in transport of cholesterol between intracellular sites and the plasma membrane for eventual removal by extracellular acceptors such as HDL is suggested.




Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
Am. J. Physiol. Cell Physiol.Home page
D. A. Prosdocimo, D. C. Douglas, A. M. Romani, W. C. O'Neill, and G. R. Dubyak
Autocrine ATP release coupled to extracellular pyrophosphate accumulation in vascular smooth muscle cells
Am J Physiol Cell Physiol, April 1, 2009; 296(4): C828 - C839.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
F. J. Field, K. Watt, and S. N. Mathur
Origins of intestinal ABCA1-mediated HDL-cholesterol
J. Lipid Res., December 1, 2008; 49(12): 2605 - 2619.
[Abstract] [Full Text] [PDF]


Home page
Physiol. GenomicsHome page
T. D. Southall, S. Terhzaz, P. Cabrero, V. R. Chintapalli, J. M. Evans, J. A. T. Dow, and S.-A. Davies
Novel subcellular locations and functions for secretory pathway Ca2+/Mn2+-ATPases
Physiol Genomics, September 14, 2006; 26(1): 35 - 45.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
H. Zheng, R. S. Kiss, V. Franklin, M.-D. Wang, B. Haidar, and Y. L. Marcel
ApoA-I Lipidation in Primary Mouse Hepatocytes: SEPARATE CONTROLS FOR PHOSPHOLIPID AND CHOLESTEROL TRANSFERS
J. Biol. Chem., June 3, 2005; 280(22): 21612 - 21621.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
P. Matarrese, A. Tinari, E. Mormone, G. A. Bianco, M. A. Toscano, B. Ascione, G. A. Rabinovich, and W. Malorni
Galectin-1 Sensitizes Resting Human T Lymphocytes to Fas (CD95)-mediated Cell Death via Mitochondrial Hyperpolarization, Budding, and Fission
J. Biol. Chem., February 25, 2005; 280(8): 6969 - 6985.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M. Walter, N. R. Forsyth, W. E. Wright, J. W. Shay, and M. G. Roth
The Establishment of Telomerase-immortalized Tangier Disease Cell Lines Indicates the Existence of an Apolipoprotein A-I-inducible but ABCA1-independent Cholesterol Efflux Pathway
J. Biol. Chem., May 14, 2004; 279(20): 20866 - 20873.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
J.-R. Nofer, R. Feuerborn, B. Levkau, A. Sokoll, U. Seedorf, and G. Assmann
Involvement of Cdc42 Signaling in ApoA-I-induced Cholesterol Efflux
J. Biol. Chem., December 26, 2003; 278(52): 53055 - 53062.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
G. Dagher, N. Donne, C. Klein, P. Ferre, and I. Dugail
HDL-mediated cholesterol uptake and targeting to lipid droplets in adipocytes
J. Lipid Res., October 1, 2003; 44(10): 1811 - 1820.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
U. Igbavboa, J. M. Pidcock, L. N. A. Johnson, T. M. Malo, A. E. Studniski, S. Yu, G. Y. Sun, and W. G. Wood
Cholesterol Distribution in the Golgi Complex of DITNC1 Astrocytes Is Differentially Altered by Fresh and Aged Amyloid beta -Peptide-(1-42)
J. Biol. Chem., May 2, 2003; 278(19): 17150 - 17157.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
M. C. Marchetti, B. Di Marco, G. Cifone, G. Migliorati, and C. Riccardi
Dexamethasone-induced apoptosis of thymocytes: role of glucocorticoid receptor-associated Src kinase and caspase-8 activation
Blood, January 15, 2003; 101(2): 585 - 593.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
W. S. Garver, K. Krishnan, J. R. Gallagos, M. Michikawa, G. A. Francis, and R. A. Heidenreich
Niemann-Pick C1 protein regulates cholesterol transport to the trans-Golgi network and plasma membrane caveolae
J. Lipid Res., April 1, 2002; 43(4): 579 - 589.
[Abstract] [Full Text] [PDF]


Home page
FASEB J.Home page
A. VON ECKARDSTEIN, C. LANGER, T. ENGEL, I. SCHAUKAL, A. CIGNARELLA, J. REINHARDT, S. LORKOWSKI, Z. LI, X. ZHOU, P. CULLEN, et al.
ATP binding cassette transporter ABCA1 modulates the secretion of apolipoprotein E from human monocyte-derived macrophages
FASEB J, July 1, 2001; 15(9): 1555 - 1561.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
B. Haidar, S. Mott, B. Boucher, C. Y. Lee, M. Marcil, and J. Genest , Jr.
Cellular cholesterol efflux is modulated by phospholipid-derived signaling molecules in familial HDL deficiency/Tangier disease fibroblasts
J. Lipid Res., February 1, 2001; 42(2): 249 - 257.
[Abstract] [Full Text]


Home page
Arterioscler. Thromb. Vasc. Bio.Home page
A. von Eckardstein, J.-R. Nofer, and G. Assmann
High Density Lipoproteins and Arteriosclerosis : Role of Cholesterol Efflux and Reverse Cholesterol Transport
Arterioscler. Thromb. Vasc. Biol., January 1, 2001; 21(1): 13 - 27.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
M. E. Brousseau, G. P. Eberhart, J. Dupuis, B. F. Asztalos, A. L. Goldkamp, E. J. Schaefer, and M. W. Freeman
Cellular cholesterol efflux in heterozygotes for Tangier disease is markedly reduced and correlates with high density lipoprotein cholesterol concentration and particle size
J. Lipid Res., July 1, 2000; 41(7): 1125 - 1135.
[Abstract] [Full Text]


Home page
J. Lipid Res.Home page
W. S. Garver, R. A. Heidenreich, R. P. Erickson, M. A. Thomas, and J. M. Wilson
Localization of the murine Niemann-Pick C1 protein to two distinct intracellular compartments
J. Lipid Res., May 1, 2000; 41(5): 673 - 687.
[Abstract] [Full Text]


Home page
J. Cell Sci.Home page
P Pujuguet, M Simian, J Liaw, R Timpl, Z Werb, and M. Bissell
Nidogen-1 regulates laminin-1-dependent mammary-specific gene expression
J. Cell Sci., January 3, 2000; 113(5): 849 - 858.
[Abstract] [PDF]


Home page
J. Lipid Res.Home page
C. G. Panousis and S. H. Zuckerman
Regulation of cholesterol distribution in macrophage-derived foam cells by interferon-{gamma}
J. Lipid Res., January 1, 2000; 41(1): 75 - 83.
[Abstract] [Full Text]


Home page
J. Lipid Res.Home page
J. F. Oram, A. J. Mendez, J. Lymp, T. J. Kavanagh, and C. L. Halbert
Reduction in apolipoprotein-mediated removal of cellular lipids by immortalization of human fibroblasts and its reversion by cAMP: lack of effect with Tangier disease cells
J. Lipid Res., October 1, 1999; 40(10): 1769 - 1781.
[Abstract] [Full Text]


Home page
BloodHome page
M. G. Cifone, G. Migliorati, R. Parroni, C. Marchetti, D. Millimaggi, A. Santoni, and C. Riccardi
Dexamethasone-Induced Thymocyte Apoptosis: Apoptotic Signal Involves the Sequential Activation of Phosphoinositide-Specific Phospholipase C, Acidic Sphingomyelinase, and Caspases
Blood, April 1, 1999; 93(7): 2282 - 2296.
[Abstract] [Full Text] [PDF]


Home page
Arterioscler. Thromb. Vasc. Bio.Home page
W. Drobnik, G. Liebisch, C. Biederer, B. Trumbach, G. Rogler, P. Muller, and G. Schmitz
Growth and Cell Cycle Abnormalities of Fibroblasts From Tangier Disease Patients
Arterioscler. Thromb. Vasc. Biol., January 1, 1999; 19(1): 28 - 38.
[Abstract] [Full Text] [PDF]


Home page
Arterioscler. Thromb. Vasc. Bio.Home page
M. Marcil, L. Yu, L. Krimbou, B. Boucher, J. F. Oram, J. S. Cohn, and J. Genest Jr
Cellular Cholesterol Transport and Efflux in Fibroblasts Are Abnormal in Subjects With Familial HDL Deficiency
Arterioscler. Thromb. Vasc. Biol., January 1, 1999; 19(1): 159 - 169.
[Abstract] [Full Text] [PDF]


Home page
J. Clin. Endocrinol. Metab.Home page
G. P. Eberhart, A. J. Mendez, and M. W. Freeman
Decreased Cholesterol Efflux from Fibroblasts of a Patient without Tangier Disease, but with Markedly Reduced High Density Lipoprotein Cholesterol Levels
J. Clin. Endocrinol. Metab., March 1, 1998; 83(3): 836 - 846.
[Abstract] [Full Text]


Home page
Arterioscler. Thromb. Vasc. Bio.Home page
A.T. Remaley, U.K. Schumacher, J.A. Stonik, B.D. Farsi, H. Nazih, and H.B. Brewer
Decreased Reverse Cholesterol Transport from Tangier Disease Fibroblasts : Acceptor Specificity and Effect of Brefeldin on Lipid Efflux
Arterioscler. Thromb. Vasc. Biol., September 1, 1997; 17(9): 1813 - 1821.
[Abstract] [Full Text]


Home page
J. Biol. Chem.Home page
J. D. Smith, M. Miyata, M. Ginsberg, C. Grigaux, E. Shmookler, and A. S. Plump
Cyclic AMP Induces Apolipoprotein E Binding Activity and Promotes Cholesterol Efflux from a Macrophage Cell Line to Apolipoprotein Acceptors
J. Biol. Chem., November 29, 1996; 271(48): 30647 - 30655.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
W. J. Johnson, R. T. Fischer, M. C. Phillips, and G. H. Rothblat
Efflux of Newly Synthesized Cholesterol and Biosynthetic Sterol Intermediates from Cells
J. Biol. Chem., October 20, 1995; 270(42): 25037 - 25046.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
A. E. Bortnick, G. H. Rothblat, G. Stoudt, K. L. Hoppe, L. J. Royer, J. McNeish, and O. L. Francone
The Correlation of ATP-binding Cassette 1 mRNA Levels with Cholesterol Efflux from Various Cell Lines
J. Biol. Chem., September 8, 2000; 275(37): 28634 - 28640.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
F. Blanchard, L. Duplomb, Y. Wang, O. Robledo, E. Kinzie, V. Pitard, A. Godard, Y. Jacques, and H. Baumann
Stimulation of Leukemia Inhibitory Factor Receptor Degradation by Extracellular Signal-regulated Kinase
J. Biol. Chem., September 8, 2000; 275(37): 28793 - 28801.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
A. J. Mendez, G. Lin, D. P. Wade, R. M. Lawn, and J. F. Oram
Membrane Lipid Domains Distinct from Cholesterol/Sphingomyelin-Rich Rafts Are Involved in the ABCA1-mediated Lipid Secretory Pathway
J. Biol. Chem., January 26, 2001; 276(5): 3158 - 3166.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. El Bawab, H. Birbes, P. Roddy, Z. M. Szulc, A. Bielawska, and Y. A. Hannun
Biochemical Characterization of the Reverse Activity of Rat Brain Ceramidase. A CoA-INDEPENDENT AND FUMONISIN B1-INSENSITIVE CERAMIDE SYNTHASE
J. Biol. Chem., May 11, 2001; 276(20): 16758 - 16766.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
E. B. Neufeld, A. T. Remaley, S. J. Demosky, J. A. Stonik, A. M. Cooney, M. Comly, N. K. Dwyer, M. Zhang, J. Blanchette-Mackie, S. Santamarina-Fojo, et al.
Cellular Localization and Trafficking of the Human ABCA1 Transporter
J. Biol. Chem., July 13, 2001; 276(29): 27584 - 27590.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1995 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement