Differentiated HL-60 cells acquire responsiveness to fMet-Leu-Phe (fMLP), which activates phospholipase C and O generation in a pertussis toxin-sensitive manner. Addition of retinoic acid (RA) for the last 24 h during dimethyl sulfoxide (MeSO)-induced differentiation enhanced fMLP-dependent signals and interaction between fMLP receptor and G. RA modifies both the function and subunit composition of G, the predominant G of HL-60 membranes, as shown by comparing purified G from membranes of MeSO-treated cells (D-G) to G from membranes of cells treated with both MeSO and RA (DR-G). As compared to D-G, DR-G induced more fMLP binding when added to membranes of pertussis toxin-treated HL-60 cells and, in the presence of GTPS, stimulated -sensitive phospholipase C in extracts of HL-60 cells to a much greater extent and at lower concentrations. Immunoblots revealed that RA induced expression of the subunit, which was otherwise undetectable in G purified from HL-60 cells or in HL-60 membranes. Possibly by inducing expression of , RA alters two functions of the G subunit, modulation of fMLP receptor-G coupling and activation of the effector, phospholipase C.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				S. M. Mervine, E. A. Yost, J. L. Sabo, T. R. Hynes, and C. H. Berlot Analysis of G Protein beta{gamma} Dimer Formation in Live Cells Using Multicolor Bimolecular Fluorescence Complementation Demonstrates Preferences of beta1 for Particular {gamma} Subunits Mol. Pharmacol., July 1, 2006; 70(1): 194 - 205. [Abstract] [Full Text] [PDF]

				V. Bertagnolo, M. Marchisio, S. Pierpaoli, M. L. Colamussi, F. Brugnoli, G. Visani, G. Zauli, and S. Capitani Selective up-regulation of phospholipase C-{beta}2 during granulocytic differentiation of normal and leukemic hematopoietic progenitors J. Leukoc. Biol., June 1, 2002; 71(6): 957 - 965. [Abstract] [Full Text] [PDF]

				G. Servant, O. D. Weiner, E. R. Neptune, J. W. Sedat, and H. R. Bourne Dynamics of a Chemoattractant Receptor in Living Neutrophils during Chemotaxis Mol. Biol. Cell, April 1, 1999; 10(4): 1163 - 1178. [Abstract] [Full Text]

				C. Montrose-Rafizadeh, P. Avdonin, M. J. Garant, B. D. Rodgers, S. Kole, H. Yang, M. A. Levine, W. Schwindinger, and M. Bernier Pancreatic Glucagon-Like Peptide-1 Receptor Couples to Multiple G Proteins and Activates Mitogen-Activated Protein Kinase Pathways in Chinese Hamster Ovary Cells Endocrinology, March 1, 1999; 140(3): 1132 - 1140. [Abstract] [Full Text]

				E. R. Neptune, T. Iiri, and H. R. Bourne Galpha i Is Not Required for Chemotaxis Mediated by Gi-coupled Receptors J. Biol. Chem., January 29, 1999; 274(5): 2824 - 2828. [Abstract] [Full Text] [PDF]

				T. Iiri, Z. Farfel, and H. R. Bourne Conditional activation defect of a human Gsalpha  mutant PNAS, May 27, 1997; 94(11): 5656 - 5661. [Abstract] [Full Text] [PDF]

				D. D. Browning, Z. K. Pan, E. R. Prossnitz, and R. D. Ye Cell Type- and Developmental Stage-specific Activation of NF-kappa B by fMet-Leu-Phe in Myeloid Cells J. Biol. Chem., March 21, 1997; 272(12): 7995 - 8001. [Abstract] [Full Text] [PDF]

				T. Iiri, P. S. Backlund Jr., T. L. Z. Jones, P. B. Wedegaertner, and H. R. Bourne Reciprocal regulation of Gsalpha by palmitate and the beta gamma  subunit PNAS, December 10, 1996; 93(25): 14592 - 14597. [Abstract] [Full Text] [PDF]