| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, July 7,
1994; and in revised form, December 21, 1994) Voltage-gated K
Volume 270,
Number 11,
Issue of March 17, 1995 pp. 6036-6041
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
Channel
Gene Transcription and Protein Expression in Pituitary Cells
channels play an essential role
in the production of action potential activity by excitable cells.
Recent studies have suggested that expression of K channel genes may be regulated by stimuli that affect electrical
activity. Elevating the concentration of extracellular KCl causes
membrane depolarization and, thus, is widely used for studying
electrical activity-dependent changes in neurons, muscle, and endocrine
cells. Here we show that elevated KCl decreases Kv1.5 K channel mRNA expression in clonal pituitary cells without
affecting Kv1.4 and Kv2.1 mRNA levels. K channel
blockers, which cause depolarization, also produce down-regulation of
Kv1.5 mRNA, while NaCl addition had no effect. Thus, the effect of KCl
is mediated by K-induced membrane depolarization.
Unlike many known effects of K, down-regulation of
Kv1.5 mRNA does not require Ca
or Na influx, or Na-H exchange.
Furthermore, the decrease in Kv1.5 mRNA expression is due to inhibition
of channel gene transcription and persists after inhibition of protein
synthesis, excluding a role for induction of intermediary regulatory
proteins. Finally, immunoblots with antibody specific for the Kv1.5
polypeptide show that depolarization for 8 h reduces the expression of
Kv1.5 channel protein. The decrease in K channel
protein expression caused by depolarization-induced
Ca-independent inhibition of Kv1.5 gene transcription
may produce a long-term enhancement of pituitary cell excitability and
secretory activity.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  W. J. Moody and M. M. Bosma Ion Channel Development, Spontaneous Activity, and Activity-Dependent Development in Nerve and Muscle Cells Physiol Rev, July 1, 2005; 85(3): 883 - 941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Krogh-Madsen, P. Schaffer, A. D. Skriver, L. K. Taylor, B. Pelzmann, B. Koidl, and M. R. Guevara An ionic model for rhythmic activity in small clusters of embryonic chick ventricular cells Am J Physiol Heart Circ Physiol, July 1, 2005; 289(1): H398 - H413. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Mali, M. Cheng, and S. K. Chintala Intravitreous Injection of a Membrane Depolarization Agent Causes Retinal Degeneration Via Matrix Metalloproteinase-9 Invest. Ophthalmol. Vis. Sci., June 1, 2005; 46(6): 2125 - 2132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-J. Fu, M.-J. Xie, L.-F. Zhang, H.-W. Cheng, and J. Ma Differential activation of potassium channels in cerebral and hindquarter arteries of rats during simulated microgravity Am J Physiol Heart Circ Physiol, October 1, 2004; 287(4): H1505 - H1515. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. I. Pozeg, E. D. Michelakis, M. S. McMurtry, B. Thebaud, X.-C. Wu, J. R.B. Dyck, K. Hashimoto, S. Wang, R. Moudgil, G. Harry, et al. In Vivo Gene Transfer of the O2-Sensitive Potassium Channel Kv1.5 Reduces Pulmonary Hypertension and Restores Hypoxic Pulmonary Vasoconstriction in Chronically Hypoxic Rats Circulation, April 22, 2003; 107(15): 2037 - 2044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Koegel, S. Kaesler, R. Burgstahler, S. Werner, and C. Alzheimer Unexpected Down-regulation of the hIK1 Ca2+-activated K+ Channel by Its Opener 1-Ethyl-2-benzimidazolinone in HaCaT Keratinocytes. INVERSE EFFECTS ON CELL GROWTH AND PROLIFERATION J. Biol. Chem., January 24, 2003; 278(5): 3323 - 3330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Conforti, M. Petrovic, D. Mohammad, S. Lee, Q. Ma, S. Barone, and A. H. Filipovich Hypoxia Regulates Expression and Activity of Kv1.3 Channels in T Lymphocytes: A Possible Role in T Cell Proliferation J. Immunol., January 15, 2003; 170(2): 695 - 702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. L. Reeve, E. Michelakis, D. P. Nelson, E. K. Weir, and S. L. Archer Alterations in a redox oxygen sensing mechanism in chronic hypoxia J Appl Physiol, June 1, 2001; 90(6): 2249 - 2256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. H. Cox, K. Folander, and R. Swanson Differential Expression of Voltage-Gated K+ Channel Genes in Arteries From Spontaneously Hypertensive and Wistar-Kyoto Rats Hypertension, May 1, 2001; 37(5): 1315 - 1322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. N. Cornfield, C. B. Saqueton, V. A. Porter, J. Herron, E. Resnik, I. Y. Haddad, and H. L. Reeve Voltage-gated K+-channel activity in ovine pulmonary vasculature is developmentally regulated Am J Physiol Lung Cell Mol Physiol, June 1, 2000; 278(6): L1297 - L1304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Hershman and E. S. Levitan RPTP{micro} and protein tyrosine phosphorylation regulate K+ channel mRNA expression in adult cardiac myocytes Am J Physiol Cell Physiol, February 1, 2000; 278(2): C397 - C403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. L. Reeve, E. K. Weir, S. L. Archer, and D. N. Cornfield A maturational shift in pulmonary K+ channels, from Ca2+ sensitive to voltage dependent Am J Physiol Lung Cell Mol Physiol, December 1, 1998; 275(6): L1019 - L1025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Wang, L. Yue, M. White, G. Pelletier, and S. Nattel Differential Distribution of Inward Rectifier Potassium Channel Transcripts in Human Atrium Versus Ventricle Circulation, December 1, 1998; 98(22): 2422 - 2428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-q. J. Liu and L. K. Kaczmarek Depolarization Selectively Increases the Expression of the Kv3.1 Potassium Channel in Developing Inferior Colliculus Neurons J. Neurosci., November 1, 1998; 18(21): 8758 - 8769. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Allen, D.-S. Koh, and B. L Tempel Cyclic AMP regulates potassium channel expression in C6 glioma by destabilizing Kv1.1 mRNA PNAS, June 23, 1998; 95(13): 7693 - 7698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-q. J. Liu and L. K. Kaczmarek The Expression of Two Splice Variants of the Kv3.1 Potassium Channel Gene Is Regulated by Different Signaling Pathways J. Neurosci., April 15, 1998; 18(8): 2881 - 2890. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D Wickenden, R. Kaprielian, Z. Kassiri, J. N Tsoporis, R. Tsushima, G. I Fishman, and P. H Backx The role of action potential prolongation and altered intracellular calcium handling in the pathogenesis of heart failure Cardiovasc Res, February 1, 1998; 37(2): 312 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. L. Muller, R. Reitstetter, and A. J. Yool Regulation of Ca2+-Dependent K+ Channel Expression in Rat Cerebellum during Postnatal Development J. Neurosci., January 1, 1998; 18(1): 16 - 25. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Van Wagoner, A. L. Pond, P. M. McCarthy, J. S. Trimmer, and J. M. Nerbonne Outward K+ Current Densities and Kv1.5 Expression Are Reduced in Chronic Human Atrial Fibrillation Circ. Res., June 19, 1997; 80(6): 772 - 781. [Abstract] [Full Text]
|
|
|
|
|
|
W. Guo, K. Kamiya, and J. Toyama Differential effects of chronic membrane depolarization on the K+ channel activities in cultured rat ventricular cells Cardiovasc Res, January 1, 1997; 33(1): 139 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Ye, S. Zangenehpour, and A. Chaudhuri Light-induced Down-regulation of the Rat Class 1 Dynein-associated Protein robl/LC7-like Gene in Visual Cortex J. Biol. Chem., August 25, 2000; 275(35): 27172 - 27176. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
