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(Received for publication, September 9, 1994; and in revised form, December 13,
1994) The outB gene of Bacillus subtilis is involved
in spore germination and outgrowth and is essential for growth. The
OutB protein was obtained by expression in Escherichia coli and purified to apparent homogeneity. Here we report experiments
showing that OutB is a NH
Volume 270,
Number 11,
Issue of March 17, 1995 pp. 6181-6185
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
-dependent NAD synthetase, the
enzyme that catalyzes the final reaction in the biosynthesis of NAD.
The enzyme is composed of two identical subunits of 30,240 Da and is
NH-dependent, whereas glutamine is inefficient as an amide
donor. The NAD synthetase is highly resistant to heat, with a half-time
of inactivation at 100 °C of 13 min. A mutant NAD synthetase was
purified from a B. subtilis strain temperature-sensitive
during spore germination and outgrowth. The mutant enzyme was 200 times
less active than the wild-type one, with a lower temperature optimum
and a non-hyperbolic kinetic versus NH
. The time course of synthesis of
OutB showed that synthesis of the enzyme started during germination and
outgrowth, and reached the highest level at the end of exponential
growth. The enzyme could be recovered from dormant spores.
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