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Volume 270, Number 12, Issue of March 24, 1995 pp. 6698-6701
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
Mussel Adhesive Plaque Protein Gene Is a Novel Member of Epidermal Growth Factor-like Gene Family (*)

(Received for publication, September 9, 1994; and in revised form, January 4, 1995)

Koji Inoue (§),  ,  Yasuhiro Takeuchi ,  Daisuke Miki ,  Satoshi Odo

From the Kamaishi Laboratories, Marine Biotechnology Institute, Heita, Kamaishi, Iwate 026, Japan

ABSTRACT

A mussel (Mytilus galloprovincialis) cDNA encoding Mgfp2, a major component of the adhesive plaque that anchors mussels tightly to underwater surfaces was isolated. It encoded a protein mainly consisted of epidermal growth factor-like repeats, containing tyrosine residues that will be converted to 3,4-dihydroxyphenylalanine near C and N termini. Amino acid residues important for cell-cell interaction in other epidermal growth factor-like proteins were, however, not conserved in the structure of Mgfp2. RNA blot analysis on adult tissues showed foot-specific expression of this gene, while the analysis on developing larvae showed that the expression starts with formation of the foot. These results suggest that the function of Mgfp2 has been specialized to form the adhesive plaque.

FOOTNOTES

*
This work was performed as a part of the Industrial Science and Technology Frontier Program supported by New Energy and Industrial Technology Development Organization. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank(TM)/EMBL Data Bank with accession number(s) D43794[GenBank].

§
To whom correspondence should be addressed: Kamaishi Laboratories, Marine Biotechnology Inst. 3-75-1 Heita, Kamaishi, Iwate 026 Japan. Tel.: 81-193-26-6544; Fax: 81-193-26-6592.

(^1)
The abbreviations used are: DOPA, 3,4-dihydroxyphenylalanine; EGF, epidermal growth factor.

(^2)
K. Inoue, Y. Takeuchi, D. Miki, and S. Odo, manuscript in preparation.

ACKNOWLEDGEMENTS

We thank Drs. J. H. Waite and S. Harayama for critical reading the manuscript, Dr. S. Miyachi for support in this study, and S. Dobashi, M. Nozawa, and Y. Mito for technical assistance.

©1995 by The American Society for Biochemistry and Molecular Biology, Inc.

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