ABSTRACT

A novel heterotrimeric G-protein -subunit has been cloned, and its function has been confirmed by expression and purification. This -subunit is only detected in the olfactory epithelium, the vomeronasal epithelium and, to a lesser extent, the olfactory bulb. It is absent from all other tissues studied including the nasal respiratory epithelium. During development, expression of G8 in the olfactory epithelium parallels neurogenesis, peaking shortly after birth and declining in the adult. In situ hybridization studies localize expression of this novel -subunit to the sensory neurons; hybridization is strongest in the region of the epithelium that contains immature neurons. Unlike proteins that are expressed only in mature olfactory neurons (e.g. olfactory marker protein or Golf), expression of G8 in the olfactory epithelium is relatively unaffected by olfactory bulbectomy. In the vomeronasal epithelium expression of G8 is also highest in the developing neurons. Taken together, these findings are consistent with a very specific role for G8 in the development and turnover of olfactory and vomeronasal neurons.

FOOTNOTES

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The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank(TM)/EMBL Data Bank with accession number(s) L35921[GenBank].

§

To whom correspondence should be addressed: Bldg. 10, Rm. 1A09, NIDR, NIH, Bethesda, MD 20892. Tel.: 301-402-2401; Fax: 301-480-8328.

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The abbreviations used are: G-protein, guanine nucleotide-binding regulatory protein; PLC, phospholipase C; OMP, olfactory marker protein; RACE, rapid amplification of cloned ends; PAGE, polyacrylamide gel electrophoresis; PCR, polymerase chain reaction; PBS, phosphate-buffered saline; Sf9, Spodoptera frugiperda clonal cell line; GTP--S, guanosine 5`-O-(3-thiotriphosphate); AEBSF, 4-(2-aminoethyl)benzenesulfonyl fluoride; DTT, dithiothreitol; PIPES, 1,4-piperazinediethanesulfonic acid; bp, base pair(s); kb, kilobase(s).

()

After the sequence of G7 was published(56) , protein sequence of another novel (but unnumbered) G-protein -subunit was reported(59) . An editorial decision was made that the protein described here should be called G8. The basis for this nomenclature is that only complete sequences of novel G-protein subunits should be numbered.

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R. Tirindelli, unpublished observation.

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