JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Samson, S. L.-A.
Right arrow Articles by Gedamu, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Samson, S. L.-A.
Right arrow Articles by Gedamu, L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 270, Number 12, Issue of March 24, 1995 pp. 6864-6871
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
Metal-responsive Elements of the Rainbow Trout Metallothionein-B Gene Function for Basal and Metal-induced Activity (*)

(Received for publication, November 14, 1994; and in revised form, January 17, 1995)

Susan L.-A. Samson (§),  ,  Lashitew Gedamu (¶)

From the Department of Biological Sciences, the University of Calgary, Calgary, Alberta T2N 1N4, Canada

ABSTRACT

In this study, the contributions of the two metal-responsive elements (MREs) of the rainbow trout (Salmo gairdnerii) metallothionein (tMT)-B gene promoter (-137 to +5) were analyzed. The effect of MRE mutations on the basal and zinc-induced activities of tMT-B promoter-reporter gene fusions were determined by transfection of a rainbow trout hepatoma (RTH-149) cell line. Together, MREa and MREb cooperate to elicit a significant response to zinc but exhibit differential basal and metal-induced activity. The MREa sequence (-62 to -51) is important for basal promoter activity and can function independently, whereas the more distal MREb (-89 to -100) mainly contributes to metal induction through cooperative interactions with MREa. The degree of basal character of the MREs is partially determined by nucleotide differences at the flexible position N of the MRE consensus TGC(G/A)CNC. In mouse L and HepG2 cells, MREa activity is conserved, but the contributions of the MREb region differ, including reduced cooperativity with MREa. There are also differences in the apparent molecular masses of the rainbow trout and mammalian nuclear factors that bind to the tMT-B promoter and MREa sequence.

FOOTNOTES

*
This study was supported in part by a Medical Research Council of Canada grant (to L. G.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
Supported by a studentship from the Alberta Heritage Foundation for Medical Research.

To whom correspondence should be addressed: Dept. of Biology, University of Calgary, 2500 University Dr. N. W., Calgary, Alberta, Canada T2N 1N4. Tel.: 403-220-5556; Fax: 403-289-9311.

(^1)
The abbreviations used are: MT(s), metallothionein(s); MRE, metal-responsive element; PCR, polymerase chain reaction; CAT, chloramphenicol acetyltransferase; LUC, firefly luciferase; tMRE, rainbow trout MT-B gene MRE.

(^2)
S. L.-A. Samson, unpublished observation.

(^3)
S. Scheiman, unpublished observation.

ACKNOWLEDGEMENTS

A portion of the site-directed mutagenesis was completed by L. G. in collaboration with Dr. J. Imbert in the laboratory of D. H. Hamer, NIH. We acknowledge the excellent technical assistance of Tapan Karchoudhury.

©1995 by The American Society for Biochemistry and Molecular Biology, Inc.

Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
N. R. Bury, M. J. Chung, A. Sturm, P. A. Walker, and C. Hogstrand
Cortisol stimulates the zinc signaling pathway and expression of metallothioneins and ZnT1 in rainbow trout gill epithelial cells
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2008; 294(2): R623 - R629.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. Viarengo, B. Burlando, M. Cavaletto, B. Marchi, E. Ponzano, and J. Blasco
Role of metallothionein against oxidative stress in the mussel Mytilus galloprovincialis
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 1999; 277(6): R1612 - R1619.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Bittel, T. Dalton, S. L.-A Samson, L. Gedamu, and G. K. Andrews
The DNA Binding Activity of Metal Response Element-binding Transcription Factor-1 Is Activated in Vivo and in Vitro by Zinc, but Not by Other Transition Metals
J. Biol. Chem., March 20, 1998; 273(12): 7127 - 7133.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. L.-A. Samson, W. J. Paramchuk, N. W. Shworak, and L. Gedamu
Functional Analyses of the Human Metallothionein-IG Gene
J. Biol. Chem., October 20, 1995; 270(42): 25194 - 25199.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1995 by the American Society for Biochemistry and Molecular Biology.