JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oliveira, C. C.
Right arrow Articles by McCarthy, J. E. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oliveira, C. C.
Right arrow Articles by McCarthy, J. E. G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 270, Number 15, Issue of April 14, pp. 8936-8943, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
The Relationship between Eukaryotic Translation and mRNA Stability
A SHORT UPSTREAM OPEN READING FRAME STRONGLY INHIBITS TRANSLATIONAL INITIATION AND GREATLY ACCELERATES mRNA DEGRADATION IN THE YEAST SACCHAROMYCES CEREVISIAE

Carla C. Oliveira , John E. G. McCarthy

A new strategy was developed to study the relationship between the translation and degradation of a specific mRNA in the yeast Saccharomyces cerevisiae. A series of 5`-untranslated regions (UTR) was combined with the cat gene from the bacterial transposon Tn9, allowing us to test the influence of upstream open reading frames (uORFs) on translation and mRNA stability. The 5`-UTR sequences were designed so that the minimum possible sequence alteration, a single nucleotide substitution, could be used to create a 7-codon ORF upstream of the cat gene. The uORF was translated efficiently, but at the same time inhibited translation of the cat ORF and destabilized the cat mRNA. Investigations of various derivatives of the 5`-UTR indicated that cat translation was primarily attributable to leaky scanning of ribosomes past the uORF rather than to reinitiation. Therefore, these data directly demonstrate destabilization of a specific mRNA linked to changes in translational initiation on the same transcript. In contrast to the previously proposed nonsense-mediated mRNA decay pathway, destabilization was not triggered by premature translational termination in the main ORF and was not discernibly dependent upon a reinitiation-driven mechanism. This suggests the existence of an as yet not described pathway of translation-linked mRNA degradation.



Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Biophys. JHome page
S. Hildebrandt, D. Raden, L. Petzold, A. S. Robinson, and F. J. Doyle III
A Top-Down Approach to Mechanistic Biological Modeling: Application to the Single-Chain Antibody Folding Pathway
Biophys. J., October 15, 2008; 95(8): 3535 - 3558.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Jurado, F. Rodriguez-Pascual, J. Sanchez-Prieto, F. M. Reimunde, S. Lamas, and M. Torres
NMDA induces post-transcriptional regulation of {alpha}2-guanylyl-cyclase-subunit expression in cerebellar granule cells
J. Cell Sci., April 15, 2006; 119(8): 1622 - 1631.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Stockklausner, S. Breit, G. Neu-Yilik, N. Echner, M. W. Hentze, A. E. Kulozik, and N. H. Gehring
The uORF-containing thrombopoietin mRNA escapes nonsense-mediated decay (NMD).
Nucleic Acids Res., January 1, 2006; 34(8): 2355 - 2363.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. Duttagupta, B. Tian, C. J. Wilusz, D. T. Khounh, P. Soteropoulos, M. Ouyang, J. P. Dougherty, and S. W. Peltz
Global Analysis of Pub1p Targets Reveals a Coordinate Control of Gene Expression through Modulation of Binding and Stability
Mol. Cell. Biol., July 1, 2005; 25(13): 5499 - 5513.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. Couttet and T. Grange
Premature termination codons enhance mRNA decapping in human cells
Nucleic Acids Res., January 23, 2004; 32(2): 488 - 494.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. L. Heikkinen, S. A. Llewellyn, and C. A. Barnes
Initiation-mediated mRNA decay in yeast affects heat-shock mRNAs, and works through decapping and 5'-to-3' hydrolysis
Nucleic Acids Res., July 15, 2003; 31(14): 4006 - 4016.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. Kebaara, T. Nazarenus, R. Taylor, A. Forch, and A. L. Atkin
The Upf-dependent decay of wild-type PPR1 mRNA depends on its 5'-UTR and first 92 ORF nucleotides
Nucleic Acids Res., June 15, 2003; 31(12): 3157 - 3165.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. H. Son, H. Jung, J. Y. Seong, Y. Choe, D. Geum, and K. Kim
Excision of the First Intron from the Gonadotropin-releasing Hormone (GnRH) Transcript Serves as a Key Regulatory Step for GnRH Biosynthesis
J. Biol. Chem., May 9, 2003; 278(20): 18037 - 18044.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Kong, X. Ji, and S. A. Liebhaber
The KH-Domain Protein {alpha}CP Has a Direct Role in mRNA Stabilization Independent of Its Cognate Binding Site
Mol. Cell. Biol., February 15, 2003; 23(4): 1125 - 1134.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. C. Oliveira, F. A. Gonzales, and N. I. T. Zanchin
Temperature-sensitive mutants of the exosome subunit Rrp43p show a deficiency in mRNA degradation and no longer interact with the exosome
Nucleic Acids Res., October 1, 2002; 30(19): 4186 - 4198.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. A. Casson, P. M. Chilley, J. F. Topping, I. M. Evans, M. A. Souter, and K. Lindsey
The POLARIS Gene of Arabidopsis Encodes a Predicted Peptide Required for Correct Root Growth and Leaf Vascular Patterning
PLANT CELL, August 1, 2002; 14(8): 1705 - 1721.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. R. Valentini, J. M. Casolari, C. C. Oliveira, P. A. Silver, and A. E. McBride
Genetic Interactions of Yeast Eukaryotic Translation Initiation Factor 5A (eIF5A) Reveal Connections to Poly(A)-Binding Protein and Protein Kinase C Signaling
Genetics, February 1, 2002; 160(2): 393 - 405.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. T. Bond, D. A. Mangus, F. He, and A. Jacobson
Absence of Dbp2p Alters Both Nonsense-Mediated mRNA Decay and rRNA Processing
Mol. Cell. Biol., November 1, 2001; 21(21): 7366 - 7379.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Z. Li, Y. Zhang, D. Li, and Y. Feng
Destabilization and Mislocalization of Myelin Basic Protein mRNAs in quaking Dysmyelination Lacking the QKI RNA-Binding Proteins
J. Neurosci., July 1, 2000; 20(13): 4944 - 4953.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. R. Cantón and P. H. Quail
Both phyA and phyB Mediate Light-Imposed Repression of PHYA Gene Expression in Arabidopsis
Plant Physiology, December 1, 1999; 121(4): 1207 - 1215.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
P. Mariottini, Z. H. Shah, J. M. Toivonen, C. Bagni, J. N. Spelbrink, F. Amaldi, and H. T. Jacobs
Expression of the Gene for Mitoribosomal Protein S12 Is Controlled in Human Cells at the Levels of Transcription, RNA Splicing, and Translation
J. Biol. Chem., November 5, 1999; 274(45): 31853 - 31862.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
P. A. Frischmeyer and HarryC. Dietz
Nonsense-mediated mRNA decayin health and disease
Hum. Mol. Genet., September 1, 1999; 8(10): 1893 - 1900.
[Abstract] [Full Text] [PDF]

Home page
 Cell Growth Differ.Home page
L. Ever, R. Steiner, S. Shalom, and J. Don
Two Alternatively Spliced Meig1 Messenger RNA Species Are Differentially Expressed in the Somatic and in the Germ-Cell Compartments of the Testis
Cell Growth Differ., January 1, 1999; 10(1): 19 - 26.
[Abstract] [Full Text]

Home page
 Microbiol. Mol. Biol. Rev.Home page
J. E. G. McCarthy
Posttranscriptional Control of Gene Expression in Yeast
Microbiol. Mol. Biol. Rev., December 1, 1998; 62(4): 1492 - 1553.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. E. Lew, S. Enomoto, and J. Berman
Telomere Length Regulation and Telomeric Chromatin Require the Nonsense-Mediated mRNA Decay Pathway
Mol. Cell. Biol., October 1, 1998; 18(10): 6121 - 6130.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
S. Mummidi, S. S. Ahuja, B. L. McDaniel, and S. K. Ahuja
The Human CC Chemokine Receptor 5 (CCR5) Gene. MULTIPLE TRANSCRIPTS WITH 5'-END HETEROGENEITY, DUAL PROMOTER USAGE, AND EVIDENCE FOR POLYMORPHISMS WITHIN THE REGULATORY REGIONS AND NONCODING EXONS
J. Biol. Chem., December 5, 1997; 272(49): 30662 - 30671.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Linz, N. Koloteva, S. Vasilescu, and J. E.G. McCarthy
Disruption of Ribosomal Scanning on the 5'-Untranslated Region, and Not Restriction of Translational Initiation per se, Modulates the Stability of Nonaberrant mRNAs in the Yeast Saccharomyces cerevisiae
J. Biol. Chem., April 4, 1997; 272(14): 9131 - 9140.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-i. Ito and M. Jacobs-Lorena
Functional Mapping of Destabilizing Elements in the Protein-coding Region of the Drosophila fushi tarazu mRNA
J. Biol. Chem., June 22, 2001; 276(26): 23525 - 23530.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1995 by the American Society for Biochemistry and Molecular Biology.