|
|
|
|||||||
|
|||||||||
Recent genetic and functional evidence suggests that the amino
terminus of the retinoblastoma (Rb) protein plays an important role in
Rb-mediated growth suppression. To explore the mechanism(s) by which
this portion of Rb may regulate cell growth, we have sought to
characterize cellular proteins that associate with the Rb amino
terminus using an in vitro protein-binding assay. Here we
report that at least one such protein is a cell cycle-regulated
Rb/histone H1 kinase (RbK) whose enzymatic and/or Rb association
activity is most prevalent in G
Volume 270,
Number 16,
Issue of April 21, pp. 9281-9288, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
/M Phases
/M phases of cells. In
contrast to previously characterized cyclin-dependent and Rb-associated
kinases, such as cdk1 (cdc2) and cdk2, G/M RbK 1) is not
depleted by incubation with p13-beads, 2) is not detected
with antisera against several Rb-associated cyclins-cdks, and 3)
associates with Rb via the Rb amino terminus, a region that is
dispensable for interaction with other Rb-associated kinases. RbK is
clearly distinct from previously characterized mitotic cdks since
cyclin A-cdc2, cyclin A-cdk2, cyclin B-cdc2, and cyclin B-cdk2 did not
associate with the Rb amino terminus. Coprecipitation experiments with
Rb antisera confirmed the association of Rb with a RbK-like kinase in
metaphase-arrested cells in vivo. Interestingly,
G
/M RbK did not appreciably associate with an analogous
portion of p107, a Rb-related protein. Taken together, these data
indicate that the Rb amino terminus specifically associates with a
novel cell cycle-regulated kinase in late cell cycle stages.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. S. Moorefield, S. J. Fry, and J. M. Horowitz Sp2 DNA Binding Activity and trans-Activation Are Negatively Regulated in Mammalian Cells J. Biol. Chem., April 2, 2004; 279(14): 13911 - 13924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Roig, M. A. Molina, A. Cascante, J. Calbo, N. Carbo, U. Wirtz, S. Sreedharan, C. Fillat, and A. Mazo Adenovirus-Mediated Retinoblastoma 94 Gene Transfer Induces Human Pancreatic Tumor Regression in a Mouse Xenograft Model Clin. Cancer Res., February 15, 2004; 10(4): 1454 - 1462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Li, K. V. Day, S. Yu, G. Shi, S. Liu, M. Guo, Y. Xu, S. Sreedharan, and B. W. O'Malley Jr. The Role of Adenovirus-mediated Retinoblastoma 94 in the Treatment of Head and Neck Cancer Cancer Res., August 15, 2002; 62(16): 4637 - 4644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yang, B. O. Williams, P. W. Hinds, T. S. Shih, T. Jacks, R. T. Bronson, and D. M. Livingston Tumor Suppression by a Severely Truncated Species of Retinoblastoma Protein Mol. Cell. Biol., May 1, 2002; 22(9): 3103 - 3110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Harbour Molecular Basis of Low-Penetrance Retinoblastoma Arch Ophthalmol, November 1, 2001; 119(11): 1699 - 1704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Harbour and D. C. Dean The Rb/E2F pathway: expanding roles and emerging paradigms Genes & Dev., October 1, 2000; 14(19): 2393 - 2409. [Full Text]
|
|
|
|
|
|
S. Bai, X. Shi, X. Yang, and X. Cao Smad6 as a Transcriptional Corepressor J. Biol. Chem., March 17, 2000; 275(12): 8267 - 8270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Sorrell, B. Combettes, N. Chaubet-Gigot, C. Gigot, and J. A.H. Murray Distinct Cyclin D Genes Show Mitotic Accumulation or Constant Levels of Transcripts in Tobacco Bright Yellow-2 Cells Plant Physiology, January 1, 1999; 119(1): 343 - 352. [Abstract] [Full Text]
|
|
|
|
 |
|
 V. L. Zacny, J. Wilson, and J. S. Pagano The Epstein-Barr Virus Immediate-Early Gene Product, BRLF1, Interacts with the Retinoblastoma Protein during the Viral Lytic Cycle J. Virol., October 1, 1998; 72(10): 8043 - 8051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Sterner, S. Dew-Knight, C. Musahl, S. Kornbluth, and J. M. Horowitz Negative Regulation of DNA Replication by the Retinoblastoma Protein Is Mediated by Its Association with MCM7 Mol. Cell. Biol., May 1, 1998; 18(5): 2748 - 2757. [Abstract] [Full Text]
|
|
|
|
|
|
B. Ouyang, H. Pan, L. Lu, J. Li, P. Stambrook, B. Li, and W. Dai Human Prk Is a Conserved Protein Serine/Threonine Kinase Involved in Regulating M Phase Functions J. Biol. Chem., November 7, 1997; 272(45): 28646 - 28651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Otterson, W.-d. Chen, A. B. Coxon, S. N. Khleif, and F. J. Kaye Incomplete penetrance of familial retinoblastoma linked to germ-line mutations that result in partial loss of RB function PNAS, October 28, 1997; 94(22): 12036 - 12040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Fan, X. Ma, B. T. Kren, M. Rice, E. B. Kmiec, and C. J. Steer A Novel Link Between REC2, a DNA Recombinase, the Retinoblastoma Protein, and Apoptosis J. Biol. Chem., August 1, 1997; 272(31): 19413 - 19417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Cuevo, S. Garrett, and J. M. Horowitz Detection and Functional Characterization of p180, a Novel Cell Cycle Regulated Yeast Transcription Factor That Binds Retinoblastoma Control Elements J. Biol. Chem., February 7, 1997; 272(6): 3813 - 3822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Wan, X. Shi, X. Feng, and X. Cao Transcriptional Mechanisms of Bone Morphogenetic Protein-induced Osteoprotegrin Gene Expression J. Biol. Chem., March 23, 2001; 276(13): 10119 - 10125. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |