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Alu sequences are interspersed throughout the genomes of primate
cells, occurring singly and in clusters around RNA polymerase
II-transcribed genes. Because these repeat elements are capable of
positioning nucleosomes in in vitro reconstitutes (Englander,
E. W., Wolffe, A. P., and Howard, B. H. (1993) J. Biol. Chem. 268, 19565-19573), we investigated whether they also
influence in vivo chromatin structure. When assayed
collectively using consensus sequence probes and native chromatin as
template, Alu family members were found to confer rotational
positioning on nucleosomes or nucleosome-like particles. In particular,
a 10-base pair pattern of DNase I nicking that spanned the RNA
polymerase III box A promoter motif extended upstream to cover diverse
5`-flanking sequences, suggesting that Alu repeats may influence
patterns of nucleosome formation over neighboring regions.
Computational analysis of a set of naturally occurring Alu sequences
indicated that nucleosome positioning information is intrinsic to these
elements. Inasmuch as local chromatin organization influences gene
expression, the capacity of Alu sequences to affect chromatin structure
as demonstrated here may help to clarify some features of these
elements.
Volume 270,
Number 17,
Issue of April 28, pp. 10091-10096, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
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