JBC Focus on PI3-Kinase with Echelon

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wu, D.
Right arrow Articles by Simon, M. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, D.
Right arrow Articles by Simon, M. I.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 270, Number 17, Issue of April 28, pp. 9828-9832, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
Different 1-Adrenergic Receptor Sequences Required for Activating Different G Subunits of Gq Class of G Proteins

Dianqing Wu , Huiping Jiang , Melvin I. Simon

In order to understand the specific interactions between receptors and guanine nucleotide-binding regulatory protein (G proteins), we attempted to delineate the 1B-adrenergic receptor sequences involved in activation of the subunits of the Gq class of G proteins. A number of specific mutations were introduced into the third inner loop of the receptor, and the mutants were tested for their abilities to activate different G subunits of the Gq class. Our results indicate that the receptor sequences required for activating Gq/11, G14, or G16 are different. The sequence extending from residues Lysto Hisis required for activation of Gq/11, but not for activation of G14 or G16. Two segments in the third loop of the receptor are required for activation of G14: one is located at the N terminus of the loop ending at residue Asn, and the other is located at the C terminus of the loop starting from residue Ser. The latter contains a BB XXB motif, which is apparently critical for G14 coupling, but not for G16 or Gq/11 coupling. Furthermore, the three amino acids stretch (Tyrto Val) included in the N-terminal segment is not only required for G14 coupling, but also for Gq/11 coupling. It may be involved to some extent in G16 coupling as well.



Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1995 by the American Society for Biochemistry and Molecular Biology.