Volume 270,
Number 18,
Issue of May 5, pp. 10365-10368, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
Laminin SIKVAV
Peptide Induction of Monocyte/Macrophage Prostaglandin E
and Matrix Metalloproteinases
Marta L.
Corcoran
,
Maura C.
Kibbey
,
Hynda
K.
Kleinman
,
Larry M.
Wahl
The laminin-derived synthetic peptide containing the SIKVAV
(Ser-Ile-Lys-Val-Ala-Val) amino acid sequence has been previously shown
to regulate tumor invasion, metastasis, and angiogenesis. Here, we
demonstate that this peptide also modulates human monocyte responses.
Moreover, the monocytic responses elicited by this peptide are
influenced by the culture conditions. When elutriated monocytes were
cultured on SIKVAV substrate or in suspension with this peptide, the
synthesis of prostaglandin E
, interstitial collagenase, and
gelatinase B was induced and was further enhanced in the presence of
concanavalin A (ConA). However, when monocytes were adhered before
adding soluble SIKVAV, the peptide alone failed to induce the
production of prostaglandin E or matrix metalloproteinases.
If adherent monocytes were exposed to SIKVAV in the presence of ConA,
this peptide enhanced the ConA induced production of these mediators.
In contrast to SIKVAV, the intact laminin molecule failed to influence
these monocyte responses. This is the first demonstration that a
laminin derived peptide is capable of inducing or enhancing monocyte
inflammatory responses that may influence a number of biological
activities such as wound healing or excessive connective tissue
destruction associated with chronic inflammation.
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