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Volume 270, Number 18, Issue of May 5, pp. 11004-11011, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.

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Sequence and Spatial Requirements for Regulated Muscle-specific Processing of the Sarco/Endoplasmic Reticulum Ca-ATPase 2 Gene Transcript

Luc Mertens , Ludo Van Den Bosch , Hilde Verboomen , Frank Wuytack , Humbert De Smedt , Jan Eggermont

Expression of the muscle-specific 2a isoform of the sarco/endoplasmic reticulum Ca ATPase (SERCA2) requires activation of an otherwise inefficient splicing process at the 3`-end of the primary gene transcript. The sequence and topology requirements for this regulated splicing event were studied in the BCH1 myogenic cell line using a minigene containing the 3`-end of the SERCA2 gene. In undifferentiated BCH1 cells, the splice process is made inefficient by the presence of a weak muscle-type 5`-donor site (5`D1) and a long terminal intron. Both optimizing the 5`D1 and decreasing the length of the muscle-specific intron, induced muscle-type splicing in undifferentiated myogenic cells. Moreover, the induction of muscle-type transcripts was only observed when two competing processing sites, the polyadenylation site (pA) used in non-muscle cells and the second neuronal 5`-donor site (5`D2), were weak. Indeed, making 5`D2 consensus induced neuronal-type splicing in undifferentiated myocytes and prevented the appearance of muscle-type transcripts. Similarly, replacing the polyadenylation site (pA) with a strong site almost completely inhibited muscle-type splicing after myogenic differentiation. We conclude that weak processing sites and a long terminal intron are required for tissue-dependent mRNA processing of the SERCA2 transcript.

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