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JBC, Vol. 270, Issue 19, 11238-11244, May, 1995
W Gu and D Reines
The rate of RNA elongation by RNA polymerase II (pol II) is affected by DNA
sequences called intrinsic arrest sites. Efficient transcription through
these sites requires elongation factor SII. In addition to the
sequence-specific features of the DNA, we show that the acquisition of
SII-dependence is a function of its "dwell-time" at an arrest site. This
temperature-dependent decay in elongation potential appears irreversible,
implying that factor-dependent and factor-independent elongation complexes
are not mutually interconvertible at this position. TFIIF and NH4Cl are
known to increase the elongation rate of pol II. Both agents preempt
arrest, consistent with the idea that elongation dwell time influences the
process. TFIIF and SII act upon different steps in a complementary way to
prevent or resolve arrest, respectively. They are probably instrumental in
facilitating the efficient transcription of large eukaryotic genes in vivo.
Identification of a decay in transcription potential that results in elongation factor dependence of RNA polymerase II
Graduate Program in Biochemistry & Molecular Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
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