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Lipopolysaccharide (LPS) is a major component of the bacterial
outer membrane, and for Rhizobium spp. has been shown to play
a critical role in the establishment of an effective nitrogen-fixing
symbiosis with a legume host. Many genes required for O-chain
polysaccharide synthesis are in the lps
Volume 270,
Number 20,
Issue of May 19, pp. 11783-11788, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
region of the
CE3 genome; this region may also carry lps genes required for
core oligosaccharide synthesis. The LPSs from several strains mutated
in the region were isolated, and their mild acid released
oligosaccharides, purified by high performance anion-exchange
chromatography, were characterized by electrospray- and fast atom
bombardment-mass spectrometry, NMR, and methylation analysis. The LPSs
from several mutants contained truncated O-chains, and the
core region consisted of a
(3-deoxy-D-manno-2-octulosomic acid)
(Kdo)-(26)-
-Galp-(16)-[
-GalpA-(14)]-
-Manp-(15)-Kdop (3-deoxy-D-manno-2-octulosomic acid)
(Kdo)pentasaccharide and a
-GalpA-(14)-[
-GalpA-(15)]-Kdop trisaccharide. The pentasaccharide was altered in two mutants in
that it was missing either the terminal Kdo or the GalA residue. These
results indicate that the lps
region, in addition to
having the genes for O-chain synthesis, contains genes
required for the transfer of these 2 residues to the core region. Also,
the results show that an LPS with a complete core but lacking an
O-chain polysaccharide is not sufficient for an effective
symbiosis.
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